Hardware Catalog SFN2019
228Pages

Twist-on efocus Miniaturized Fluorescence Microscope

Contents Light Generation & Control LED Illumination . . . . . . . . . . . . . . . . . . . . Laser Diode Illumination . . . . . . . . . . . . . . . Ce:YAG Fluorescent Illumination . . . . . . . . . . . Modulators . . . . . . . . . . . . . . . . . . . . . . . Beam Splitters/Combiners Doric Mini Cubes . . . . . . . . . . . . . . . . . . . Doric Micro Splitters . . . . . . . . . . . . . . . . . Doric Multiple Splitters/Combiners . . . . . . . . . Beam Modifiers Filtering . . . . . . . . . . . . . . . . . . . . . . . . . NA Converter . . . . . . . . . . . . . . . . . . . . . Rotary Joints...

Fiber Photometry Console . . . . . . . . . . . . . . . . . . . . . . . . . Connectorized Fluorescence Mini Cubes . . . . . . . . . . . . . . . . . Integrated Fluorescence Mini Cubes . . . . . . . . . . . . . . . . . . . Rotary Fluorescence Mini Cubes . . . . . . . . . . . . . . . . . . . . . . Bundle-imaging Fluorescence Mini Cubes . . . . . . . . . . . . . . . . Photodetectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fiber Photometry Accessories . . . . . . . . . . . . . . . . . . . . . . . Behavioral Tracking Behavior Tracking Cameras . . . . . . . . . . . . . . . . ....

Overview of Neuro-photonics products catalog Over the years, this catalog has grown beyond its optogenetics roots to become a reference for neuro-photonics products. Initially, it covered only the hardware that was used for light stimulation and/or control of cells marked with genetically encoded light-sensitive proteins. In these experiments, the light from a laser or an LED source is sent through an optical fiber to a slice of brain tissue, or to the brain of a head-fixed or freely-moving animal. Overtime, this simple optical link has evolved into more complex circuitry, resembling the...

(Left) Optogenetic stimulation with a Laser Diode Fiber Light Source. (Right) Bilateral optogenetic stimulation with a Connectorized LED with Fiber-optic Rotary Joint.

(Left) Dual optogenetic stimulation with a Laser Diode Fiber Light Source. (Right) 2-color optogenetic stimulation with a Ce:YAG + LED Fiber Light Source.

(Left) Miniaturized Fluorescence Microscopy System used for calcium imaging with GCaMP6. (Right) Fiber Photometry System with a 4 port Integrated Fluorescence Mini Cube and a Fiber Photometry Console.

(Left) Optogenetic stimulation and electrophysiology recordings with an Opto-electric Probe Tip. (Right) Optogenetic stimulation and fluid delivery with a Mono Opto-fluid Cannula

Fiberless & Wireless Optogenetically Synchronized Electrophysiology System and Behavior Tracking Camera

Light Generation & Control LED Illumination Light emitting diodes (LEDs) coupled in an optical fiber are suitable for neuroscience experiments which need to bring light into the brain. LED light allows to control the excitation, inhibition or signalling of specific cells in optogenetic experiments. The uniform illumination of an LED makes it the preferred light source for miniature fluorescence microscopy and fiber photometry. Our compact Connectorized LEDs or multiple color combined LEDs are used with programmable LED Drivers. We also offer LED Fiber Light Sources integrating 1, 2, or 4...

Light Generation & Control Table 1: Typical Connectorized LED Output Power vs Optical Fiber Core Diameter Overdrive @2000 mA (pulsed) TYPICAL OUTPUT POWER @1000 mA (mW)* The power is given for Connectorized LEDs and LEDs with Fiber-optic Rotary Joints (LEDFRJ)† . Contact us for power levels for other LED products. Connectorized LED male pinout power values taken at a maximum current of 1000 mA, except for 365, 385, 405 and 420 nm LEDs (500 mA). In overdrive mode, LED drivers can produce

Light Generation & Control 13 Table 2: Connectorized LEDs Color Codes Central Wavelength (nm) ORDERING CODE: CLED LED color code (see Table 2) LED Drivers Doric programmable LED Drivers are available in 1-, 2-, and 4-channel versions. When connected to a Connectorized LED having an eprom memory, the LED Driver recognizes the LED wavelength and automatically sets the maximum current value. In stand-alone mode, all LED Drivers allow cw operation and external analog modulation through an input BNC connector for each channel. For each channel, there is also a current monitoring BNC output...

Light Generation & Control eliminating the need for a function generator. In low-duty cycle pulsed mode, the software allows to overdrive the LED sources if a higher power is needed. For multiple channel driver versions, each channel is controlled independently. Although not mandatory for LED sources, our LED Drivers come with a safety interlock connector and a main key switch. These safety features are of interest for UV and near infrared LEDs. Note: The renewed line of Doric LED Drivers has a new connector pinout that does not include pins for fan power. It is thus essential to use a Fan...

Light Generation & Control Combined LEDs Doric Combined LEDs merge the light from multiple LEDs of different colors into a single output connector by using a patent pending regular pentagon mirrors configuration. The coupling efficiency for respective colors is near those of our Connectorized LEDs. Each LED of the Combined LEDs is driven independently via M8 cable when connected to any of our driver(s). Notes: • A compatible holder is included to secure the Combined LEDs. • Combined LEDs do not include the corresponding LED Driver. See Table 4 for available LED Driver models. • The renewed line...

Light Generation & Control Notes: • A compatible holder is included with the Connectorized LED + Fiber-optic Rotary Joint (Holder FRJ large; see Table 117). • An optional gimbal holder allows pivoting the rotary joint along two additional axes, further reducing the mechanical stress on the animal (GH FRJ; see Table 119). • A Connectorized LED with Fiber-optic Rotary Joint does not include the corresponding LED Driver. See Table 4 for available LED Driver models. ORDERING CODE: LEDFRJ LED color code (see Table 2) Combined LEDs with Fiber-optic Rotary Joint Combined LEDs with Fiber-optic...