In Vitro ADME Discovery Screening Research Services
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In Vitro ADME Discovery Screening Research Services The most rapid path to more sound decision making

The information you need to make important drug discovery decisions. Drug discovery screening requires making choices that can dramatically affect the success of your business well into the future. With the proper information, you can become more efficient and more assured you are proceeding on a viable path in pursuit of the next treatment or cure. SM That is why Corning® Gentest Contract Research Services delivers a unique combination of industry leading proprietary products, advanced technology, personalized guidance from expert study directors and reliable, submission-ready results....

THE MOST rapid path to more sound decision making Tested Science. Reliable Results. Drug developers now require faster and more predictive compound screens to reduce time-to-market and potential drug-drug interactions. Corning Life Science’s ADME Contract Research Services group works as an extension of a client’s team to provide reliable data and faster results. Utilizing state-of-the art techniques, products, and equipment, Corning is able to assist clients in screening for viable drug candidates during drug discovery or by preparing regulatory agency submission-quality reports for your...

Enzyme Induction Studies with Cytochrome P450 and UGT Induction Induction of cytochrome P450 and UGT enzyme catalytic activity is a leading mechanism of metabolism-based drug-drug interactions. In vitro induction studies are valuable predictors of in vivo drug-drug interactions and the magnitude of that interaction. Testing for enzyme activity, mRNA or western blotting for a comprehensive portfolio of inducible enzymes, including CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP3A4, UGTs Fully validated LC/MS/MS analytical methods for the major inducible CYPs in enzyme activity assays...

Extensive Suite of Assays For more than ten years, Corning has been providing regulatory agency submission-quality enzyme induction services in human hepatocytes. Corning induction assays go beyond the standard CYP1A2, 2B6 and 3A4 tests. Additional enzymes that may be George Zhang requested by regulatory agencies are available with enzyme STUDY DIRECTOR activity, mRNA or western blot endpoints, including CYP2C8, CYP2C9, CYP2C19, UGTs and transporters. Assay Flexibility and Customization Corning provides induction assays using both cryopreserved and With over 15 years of research experience,...

High-throughput CYP Inhibition service with FDA-recommended probe substrates Inhibition of cytochrome P450 enzyme catalytic activity is a leading mechanism of metabolism-based drug-drug interactions. In vitro inhibition studies are valuable predictors of in vivo drug-drug interactions and the magnitude of that interaction. In many cases, this information can eliminate the need for further in vivo studies. The in vitro ADME market leader in P450 products and services, Corning, and the technology leader in high-throughput mass spectrometry, Agilent Technologies, are combining expertise to...

REACTION PHENOTYPING expertise Enzyme identification by substrate loss analysis using Corning® Supersomes™ enzymes Reaction phenotyping studies help identify the number and identity of P450, UGT or other enzyme-mediated pathways of elimination—important information that affects population variability in metabolism and the risk of becoming a victim drug in a drug-drug interaction event. Using Corning Supersomes, the gold standard for recombinant metabolizing enzymes, is a key element to delivering reproducible results. Enzyme concentrations for specific enzymes are fixed or scaled to provide...

PERMEABILITY AND DRUG TRANSPORT expertise P-gp Interaction Assessment with Caco-2 or MDR1-LLC-Pk1 Cell Monolayers Bidirectional transport assays across Caco-2 and MDR1-LLC-PK1 monolayers comply with the FDA recommended approach to determine apparent permeability of a test article and assess P-glycoprotein (P-gp) mediated transport and inhibition. SM Only Corning® Gentest Contract Research Services delivers a combination of industry leading proprietary products, advanced technology, expert guidance from renowned Study Directors and reliable, submission ready results. Together, these elements...

Efflux Transporter Activity in Caco-2 and LLC-Pk1 Cell Monolayers P-gp, BCRP, and MRP2 transporter activity assessment in Caco-2 and LLC-PK1 cell monolayers Data represents efflux ratios of each probe substrate and the effects of prototypical inhibitors on the activity of each transporter. Efflux ratios were generated from mean A-B and B-A Papp values of duplicate monolayers. Efflux of the P-gp probe substrate digoxin was observed in both Caco-2 and MDR1-LLC-PK1 cells, and can be inhibited by the known P-gp inhibitors ketoconazole, quinidine, and verapamil. Efflux of the BCRP probe...

METABOLIC STABILITY expertise Metabolic stability assays measure the stability of a test compound by incubating the test compound with liver microsomes, hepatocytes, or S9 enzyme sources from human and animal species. Evaluating primary metabolism and pharmacokinetics in the liver helps identify whether a compound is chemically stable and metabolized at an acceptable rate within the body. Corning provides rapid in vitro metabolic stability testing using several different enzyme sources. Most often, this test utilizes hepatocytes or liver microsomes. Corning’s metabolic stability testing...

Historical Assay Performance for Metabolic Stability in Human, Rat, and Mouse Liver Microsomes Corning Study Directors have immediate access to high-quality Corning products for conducting studies, including Corning® Gentest™ HLMs, hepatocytes, chemicals and Corning Supersomes™, the industry gold standard for recombinant enzymes and the full portfolio of Corning cell culture products—enabling trusted science and reliable results. Corning Gentest CryoHepatocytes 100 Corning Gentest metabolism-qualified human cryohepatocytes are suitable for in vivo like metabolic stability and drug clearance...