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Stem Cell Research

Stem Cell Research
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Stem Cell Research

Product catalog summary
Overview
This document serves as a comprehensive guide to Corning's solutions for stem cell research, detailing products and technologies that support the entire workflow from isolation to storage.
Specifications and Products
  • Isolation and Derivation: Corning provides advanced surfaces, cell strainers, centrifuge tubes, and reagents like the SMC4 Small Molecule Cocktail to enhance iPSC reprogramming and cell recovery.
  • Expansion: Includes the FloWell Perfusion Plate, Matrigel Matrix, Synthemax Surface, and various media and vessels for scalable cell expansion.
  • Differentiation: Offers extracellular matrices, growth factors, and ultra-low attachment surfaces to optimize cell differentiation.
  • Analysis: Tools include biologically coated microplates, Matrigel Matrix, and PuraMatrix Peptide Hydrogel for in vitro and in vivo analysis.
  • Harvest: Equipment and reagents such as cell recovery solutions, Dispase, and Trypsin EDTA for efficient cell harvesting.
  • Storage: Cryogenic vials, bags, and the SMC4 Small Molecule Cocktail ensure cell viability during storage and recovery.
Procedures and Recommendations
  • Emphasizes the use of defined, xeno-free conditions for stem cell culture to ensure reproducibility and safety.
  • Products support both basic and clinical research applications but are not intended for diagnostic or therapeutic use in humans.
Conclusion
Corning offers a wide array of products and technologies to support the entire stem cell research process, ensuring high-quality and scalable solutions for researchers.
Additional Information
  • Materials and Equipment: Lists essential items such as DMSO, media, sera, reagents, pipet tips, and pipettors necessary for stem cell research.
  • Product Specifications: Detailed descriptions of Corning products, including peptide hydrogels, growth factors, and culture supplements with catalog numbers and packaging details.
  • Culture Vessels: Information on culture dishes, flasks, and plates, including ultra-low attachment options and ECM mimetic surfaces.
  • Growth Factors and Supplements: Lists growth factors like NGF, bFGF, EGF, and others for differentiation and expansion of stem cells.
  • Cryogenic Storage Solutions: Details on cryogenic vials and storage boxes for safe storage of biological samples.
  • Ordering Information: Provides catalog numbers, descriptions, and packaging details for easy ordering.
  • Contact Information: Lists worldwide support offices for customer service and technical support.
Critical Information: All products are for research use only and not intended for human use or diagnostic procedures. Contact details for Corning's global support offices are included.
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Catalog excerpts

Stem Cell Research-3

BEGINNING-TO-END SOLUTIONS FOR STEM AND PRIMARY CELL RESEARCH The science of stem cell culture has advanced rapidly since its beginnings in the 1980s, as has the technology behind this research. From feeder-free substrates, to defined media, to scalable cell expansion systems, continual advances in stem cell culture have inspired Corning to develop i nnovative new tools to support this groundbreaking work. Corning was a leader in disposable cell cultureware during the exciting early days of stem cell culture. Today, we continue to work with researchers, providing high quality cell culture consumables,...

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Stem Cell Research-4

Classical Media Serum Free • DMEM with high glucose • DMEM with low glucose • DMEM with glutagro™ Supplement • MEM • MEM Alpha • IMDM • RPMI • Fetal Bovine Serum (FBS), Regular and Premium • Heat Inactivated FBS • Specialty FBS • Human AB Serum • ITS • MITO • Delipidized albumin • Growth factors • Trace elements A, B, C • stemgro® hMSC Medium and Supplement • Recombinant growth factors • Recombinant albumin • Recombinant lactoferrin Vessels for Stem Cell CultureSimplify scale-up with Corning culture vessels designed for each stage of the cell culture workflow. vy For attachment-dependent cells:...

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Stem Cell Research-5

Corning Products for Isolation and Derivation w Advanced surfaces Cell strainers and centrifuge tubes Pipet tips and pipettors Pipets and pipet controllers Corning® SMC4 Small Molecule Cocktail Syringe and filter systems Tissue homogenizers Products for primary or stem cell isolation and derivation include tools for tissue extraction, ECMs for isolation of primary or progenitor cells, reagents for enhancing iPSC reprogramming, and novel vessels for continuous feeding during prolonged derivation and differentiation protocols. 1. Corning SMC4 Small Molecule Cocktail: an optimized supplement of...

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Stem Cell Research-6

EXPANSION Corning Products for Expansion w Corning® FloWell™ 2W Perfusion Plate Corning Matrigel® Matrix and Defined Extracellular Matrices Corning Synthemax® Surface and Corning PureCoat™ ECM Mimetic Surfaces Dishes, flasks, and plates Media, serum, and serum-free supplements Multi-layer vessels, including the Falcon® Multi-Flask, Corning HYPERFlask,® CellSTACK® and HYPERStack® product lines. Corning stemgro® hMSC Medium and Supplement Novel and scalable environments for stem and primary cell types for cell expansion in basic and c linical research applications. 1. Corning Matrigel Matrix: The...

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Stem Cell Research-7

3. Corning® FloWell™ 2W Plate: Multiple well plate designed with continuous fluid flow technology for controlled feeding of cells for up to 72 hours. Ideal cell feeding system for protocols that require frequent medium changes, such as during pluripotent stem cell induction and subsequent PSC expansion. Compatible with both Corning Matrigel® Matrix and Corning Synthemax® Surface self-coating protocols. 4. Multi-layered Flasks: Choose from a variety of sizes and surfaces, including 3- and 5-layer Falcon® Multi-Flasks with tissue culture treated surfaces or Corning PureCoat™ ECM Mimetic Fibronectin...

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Stem Cell Research-8

Corning Products for Differentiation w Corning® Matrigel® Matrix and defined extracellular matrices Dishes, flasks, and plates Growth factors, serum-free media supplements Media, serum, and reagents Multilayer vessels including the Falcon® Multi-Flask, Corning HYPERFlask® and HYPERStack® product lines Ultra-Low Attachment plates, dishes and flasks Optimize the cell culture environment to direct cell differentiation and specialization with Corning’s tools and technologies. 1. Corning Extracellular Matrices: Choose from a wide variety of animal- or human-derived biological ECMs as well as synthetic...

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Stem Cell Research-9

ANALYSIS Corning Products for Analysis w Biologically coated micro plates in HTS formats Corning® Matrigel® Matrix, high concentration (in vivo delivery) Corning PuraMatrix™ Peptide Hydrogel (in vivo delivery) Corning Osteo Assay Surfaces Dishes, flasks, and plates Media, sera, reagents Corning Transwell® Permeable Supports Pipet tips and pipettors Pipets and pipet controllers Ultra-Low Attachment plates, dishes, flasks (embryoid bodies) Tools for in vitro and in vivo analysis of stem, progenitor, and differentiated cell types. In vivo 1. Corning Matrigel Matrix, high concentration (HC): Biocompatible...

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Stem Cell Research-10

Corning Products for Harvest w Benchtop equipment Cell dissociation reagents Buffered salt solutions Cell scrapers Centrifuge tubes and cell strainers Pipet tips and pipettors Pipets and pipet controllers Syringe and filter systems Pipets, centrifuge tubes, cell scrapers, and reagents to make cell harvesting easier. 1. Corning® Cell Recovery Solution: A non-enzymatic cell recovery solution recommended for recovering cells from Corning Matrigel® Matrix without damaging membrane-bound receptors, or cell:cell interactions. 2. Corning Dispase: An animal-free, bacillus-derived, neutral metallo rotease...

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Stem Cell Research-11

Corning® Products for Storage w Centrifuge tubes Cryogenic bags, vials and accessories DMSO (Dimethyl Sulfoxide) Media, sera, reagents Pipet tips and pipettors Pipets and pipet controllers Corning SMC4 Small Molecule Cocktail Storage Solutions for Stem Cell Culture Minimize the stresses on cultures during storage and maximize their subsequent recovery and s urvival with Corning storage solutions. 1. Cryogenic vials and accessories: Corning offers a variety of externally and internally threaded vials, as well as externally threaded vials with a plug seal cap. All vials are sterilized by gamma...

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Stem Cell Research-12

354250 Corning® PuraMatrix™ Peptide Hydrogel, 5 mL 354248 Corning Matrigel® Basement Membrane Matrix, High Concentration (HC), LDEV-Free, 10 mL 354005 Corning 2.5S Nerve Growth Factor (NGF), Mouse Natural, 10 pg 356005 Corning 2.5S Nerve Growth Factor (NGF), Mouse Natural, 1 mg, 2 x 500 pg 356004 Corning 2.5S Nerve Growth Factor (NGF), Mouse Natural, 100 pg 354009 Corning 7S Nerve Growth Factor (NGF), Mouse Natural, 100 pg 356037 Corning Basic Fibroblast Growth Factor (bFGF), Bovine Natural, 10 pg 354060 Corning Basic Fibroblast Growth Factor (bFGF), Human Recombinant, 10 pg 356060 Corning Basic...

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