Corning® TransportoCells™ Cryopreserved Transporter Cells Corning TransportoCells products are high performance mammali­ n cells in a convenient, cryopreserved format that a transiently overexpress a single human SLC transporter protein. The frozen cells deliver robust data, while eliminating the time required to culture and maintain stable cell lines. Culturing and maintaining stable transporter cell lines can be expensive and time-consuming. In addition, it can take a week or more to prepare cells for assaying. Corning TransportoCells products can be thawed, plated and assayed in just two days with high uptake ratios. Cryopreserved format provides flexibility for experimental ­planning Cells are readily available, can be stored onsite and shipped globally Cells can be thawed, plated and assayed in just two days Robust results with uptake ratios ≥10 fold Consistent with other mammalian cell-based models One vial contains 10 million cells and supports one 24-well or one 96-well plate Includes all current USFDA and European Medicines Agency (EMA)-recommended SLC drug transporters Convenient and Cost-efficient Corning TransportoCells products are a convenient, cost-efficient alternative to maintaining stable cell lines. They provide the utmost flexibility for experimental planning. The cells can be removed from storage one day and assayed the following day. Robust and Validated Corning TransportoCells products deliver high performance and robust data with uptake ratios ≥10 fold. The model has been fully validated for substrate specificity, transporter kinetics and inhibition profiles to ensure data are consistent with existing transporter cell models. Supports Regulatory Recommendations Corning TransportoCells products support USFDA and EMA recommendations for identification of drug transporters and transporter drug-drug interaction studies critical in the development of new investigational drugs. Contract Research Services Available SLC Transporter Interaction Studies using Corning TransportoCells products are available from Corning GentestSM Contract Research Services. All assays are designed and built to meet regulatory agency ­recommendations.

Table 1. Performance Summary of Corning® TransportoCells™ Cryopreserved SLC Transporter Cells. The post thaw viability exceeds 80%. Uptake activity of HEK293 cells transiently over-expressing OATP1B1*1a (wild-type), OATP1B3, OAT1, OAT3, OCT1 and OCT2, respectively, are evaluated by incubating the cells with listed prototypical substrates at indicated concentration. Uptake ratio is calculated by dividing uptake activity measured in the SLC transporter cells by that in control cells. Uptake Activity in Transporter Cells (pmol/mg/min) Uptake Activity in Control Cells (pmol/mg/min) Uptake Ratio...

Corning® TransportoCells™ Human Cryopreserved SLC Transporter Cells Ordering Information Cat. No. Full Name Gene Accession Number Organic anion-transporting polypeptide 1B1 Wild Type (388A) Organic anion-transporting polypeptide 1B3 Organic anion transporter 1 Organic anion transporter 3 Organic cation transporter 1 Organic cation transporter 2 Related Products and Contract Research Services w ABC Human and Animal Transporter Membranes and Vesicles BCRP/MRP, and BSEP Vesicle Assay Kits Corning GentestSM Contract Research Services – assays designed and built to meet regulatory agency recommendations...