Corning® Tissue Fractions Reagents for Drug Metabolism

Corning® Tissue Fractions Reagents for Drug Metabolism Tissue fractions from human and animal livers represent an important tool in preclinical metabolism studies for predicting the toxicity and pharmacokinetic properties of a drug compound. Liver microsomes from humans and animal species play an important role in evaluating drug compounds for metabolic stability, reactive metabolites, drug-drug interactions, reaction phenotyping, and metabolite identification. For years, Corning has been a trusted provider of a wide range of high-quality tissue fractions, including liver and intestinal microsomes,...

REPRODUCIBILITY Corning® Gentest™ Human Liver Microsomes Corning provides human subcellular fractions, as well as a variety of animal models. Each product contains an average representative pool of donors. Corning UltraPool™ HLM 150 is fully characterized (Km and Vmax) according to GLP standards for major cytochrome P450 activities and select phase II enzymes using FDA-recommended substrates. Corning Gentest and Corning UltraPool human liver microsomes are readily available as individual products, complete kits, or large lots for global supply. Characterized for a range of enzymes Corning Gentest...

Corning® Gentest™ Pooled HLMs Portfolio 20 Donors Catalog Number 452161 (single vial) 452155 (80 vials in easy-count box) 452961 (S9) 452156 (single vial) 452152 (80 vials in easy-count box) 452227 (1 vial each, HLM, S9, Cytosol) 452117 (single vial) 452118 (80 vials in easy-count box) 452116 (S9) 452115 (Cyotsol) Donor Number Storage Buffer Gender Ratio Donor Blend Method Pre-selected donors mixed at ratios to give CYP activities for big 5 P450s (CYP1A2, 2C9, 2C19, 2D6, and 3A4) Equal mix of donors on a per mg microsomal protein basis Equal mix of donors on a per mg microsomal protein basis...

CONSISTENCY Corning® UltraPool™ HLM 150— Consistent and rapid results Corning UltraPool HLM 150 is the first commercially available large donor pool. Corning UltraPool HLM 150 enables consistency in experimental results in multi-site and long-term programs by delivering reproducible data across multiple CYP and UGT enzymes as compared to smaller pools of donors. High degree of lot-to-lot consistency for CYP and UGT activity Corning UltraPool HLM 150 pool is statistically modeled so lot-to-lot variability represented as coefficient of variation (CV) is <10% (mean) for the more variable CYP enzyme...

Lot-to-lot Consistency Monte Carlo Statistical Analysis 50 Monte Carlo, mean (n=30) 9%_6%_17%_10%_12% Mean, standard deviation and calculated CVfor CYP activity is from HLM isolated from over 300 donors. CV decreases as the pool size increases and tends to level off after 150 donors. A reduction in CV as the donor number increases applies to all metabolic enzymes. The data shown above resulted from a Monte Carlo analysis modeling the expected vari- ability in HLM pools of differing sizes. Variability is measured by CV. Kinetic Constants for CYP Isoform Activities for Assay Protein Assay Vmax...

Corning Gentest HLMs Additional options to enhance flexibility Mixed Gender 50-Donor HLM Pool The Corning Gentest mixed gender 50-donor HLM pool is designed to improve predictions and scaling by minimizing metabolizing enzymes donor variability. The mixed gender pool is an equal mix of donors on a per mg microsomal protein basis as well as an equal ► Western Blot testing for CYP3A5 and CYP3A4 ► Activities for key CYP, FMO, and UCT isoforms are equal to an average patient Mixed Gender 20-Donor HLM Pool The Corning Gentest mixed gender 20-donor HLM pool is formulated to represent an aver- age profile...

SPECIALIZED Corning® Specialty Tissue Fractions Additional options to enhance flexibility Corning Life Sciences’ portfolio of specialty pooled and single donor tissue fractions are designed to address differences in populations, catalytic activity, and genetic polymorphism. They are all highly characterized for catalytic activity measuring important metabolizing CYP, UGT, and other enzymes. Specialty Human Pooled Tissue Fractions Corning Life Sciences’ specialty pooled products are formulated to address metabolic differences between population groups. The portfolio of specialty tissue fractions...

SPEED Corning® Animal Tissue Fractions Designed to speed metabolism studies Corning Life Sciences’ animal subcellular fractions are prepared with the same quality, reproducibility, and characterization as our human liver products. Like human liver products, animal tissue fractions are well suited for metabolic stability testing, metabolite identification, and mechanistic studies. Animal Liver Tissue Fractions Comparing in vitro profiles can aid in selecting a preclinical model for your in vivo studies. Corning Gentest™ animal tissue fractions can be used to quickly generate metabolism data such...

SERVICE AND SUPPORT In Vitro ADME/Tox Services Corning Life Sciences is dedicated to the development of novel assay solutions for ADME/ Tox research, including products and research services that are backed by a world-class service and support team. For over 25 years, Corning has actively worked with ADME/Tox researchers to develop tools that help improve workflow, ease of use, and performance. This in-depth knowledge and experience is available to customers through application and technical support and expert contract research services. Technical Applications Support Corning Life Sciences technical...

HLM Product List Corning® UltraPool™ HLM 150 Tissue Fractions – HLMs, S9, and Cytosol isolated from the same 150 donors Cat. No. Description Qty. Protein Concentration Mixed Gender Pooled 150-donor HLM Mixed Gender Pooled 150-donor HLM (80 vials of 452117 packaged in an easy-count box) Mixed Gender Pooled 150-donor S9 Mixed Gender Pooled 150-donor Cytosol Corning Gentest™ Human Mixed Pooled HLMs, S9, and Cytosol Cat. No. Description Qty. Protein Concentration Mixed Gender Pooled 50-donor HLM Mixed Gender Pooled 50-donor HLM (80 vials of 452156 packaged in easy-count box) Human 50-donor Tissue...