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Corning® Spin-X® UF Concentrator Selection and Use Guide

Corning® Spin-X® UF Concentrator Selection and Use Guide
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Corning® Spin-X® UF Concentrator Selection and Use Guide - 1

Corning® Spin-X® UF Concentrator Selection and Use Guide Contents Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Choosing the Right Concentrator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Choosing the Best Molecular Weight Cut-off Membrane . . . . . . . . . . . . . . . . 3 Helpful Hints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Chemical Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 2

Introduction Corning® Spin-X® UF concentrators are disposable, single use only ultrafiltration devices with polyethersulfone membranes (PES) for the centrifugal concentration and/or purification of biological samples. This guide will help you chose the best Spin-X UF concentrator for your application. Major Uses for Ultrafiltration Ultrafiltration is a convective process that uses anisotropic semi-permeable membranes to separate macromolecular species and solvents primarily on the basis of size. It is particularly appropriate for the concentration of macromolecules and can also be used to...

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 3

Spin-X UF 20 mL centrifugal concentrators have been developed to offer increased volume flexibility and performance. Spin-X UF 20 handles up to 20 mL in swing bucket centrifuges and 14 mL in 25° fixed angle rotors accepting 50 mL centrifuge tubes. Featuring twin vertical membranes for unparalleled filtration speeds the Spin-X UF 20 can achieve lOOx plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipet recovery of the final concentrate. Table 2. Technical Properties of...

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 4

The advanced designs and low adsorption materials that characterize Corning® Spin-X® UF products offer a unique combination of faster processing speeds and higher recovery of the concentrated sample. Providing that the appropriate device size (Table 2) and membrane cut-off (Table 3) is selected, Corning Spin-X UF products will typically yield recoveries of the concentrated sample in excess of 90% when the starting sample contains over 0.1 mg/mL of the solute of interest (Table 4). Most of the loss is caused by nonspecific binding both to the membrane surface and to exposed binding sites on...

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 5

Helpful Hints Flow Rate Filtration rate is affected by several parameters, including MWCO, porosity, sample concentration, viscosity, centrifugal force and temperature. Expect significantly longer spin times for starting solutions with over 5% solids. When operating at 4°C, flow rates are approximately 1.5 times slower than at 25°C. Viscous solutions such as 50% glycerin will take up to 5 times longer to concentrate than samples in a predominantly buffer solution. Prerinsing Membranes fitted to Spin-X® UF concentrators contain trace amounts of glycerin and sodium azide. Should these...

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 6

Chemical Compatibility Spin-X UF® concentrators are designed for use with biological fluids and aqueous solutions. For chemical compatibility details, refer to Table 6. Table 6. Chemical Compatibility* (2 hr contact time; compatible pH range, pH 1-9) Acetic Acid (25.0%) 1 Lactic Acid (5.0%) 1 Ammonium Hydroxide (5.0%) 2 Nitric Acid (10.0%) 1 Ammonium Sulphate (saturated) 1 Phenol (1.0%) 2 Dimethyl Formamide (10.0%) 2 Sodium Carbonate (20%) 2 Dimethyl Sulfoxide (5.0%) 1 Sodium Deoxycholate (5.0%) 1 Ethanol (70.0%) 1 Sodium Dodecylsulfate (0.1 M) 1 Ethyl Acetate (100%) 3 Sodium Hydroxide 3...

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 7

Corning® Spin-X® UF Concentrator Ordering Information Cat. No. Description Capacity Membrane Pack Size CORNING SPIN-X UF CONCENTRATOR SELECTION AND USE GUIDE

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Corning® Spin-X® UF Concentrator Selection and Use Guide - 8

www.corning.com/lifesciences Corning and Spin-X are registered trademarks of Corning Incorporated, Corning, NY. All other trademarks are the property of their respective owners. Corning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001 EUROPE France t 0800 916 882 f 0800 918 636 Germany t 0800 101 1153 f 0800 101 2427 The Netherlands t 31 20 655 79 28 f 31 20 659 76 73 United Kingdom t 0800 376 8660 f 0800 279 1117 All Other European Countries t 31 (0) 20 659 60 51 f 31 (0) 20 659 76 73 L AT I N A M E R I C A Brasil t (55-11) 3089-7419 f (55-11) 3167-0700 Mexico t (52-81) 8158-8400...

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