Corning® Gentest™ High Viability CryoHepatocyte Recovery Kit The Corning Gentest high viability cryohepatocyte recovery kit provides a high-viability, high-yield recovery medium optimized for a single centrifugation step as well as an optimized plating medium for high plating efficiency. Features w Improves cryopreserved hepatocyte cell viability, yield, and plating efficiency w Saves researchers time with an optimized single centrifugation recovery step versus traditional two-step processes w Provides recovery medium for two purifications; up to five vials of cryopreserved hepatocytes can be processed per purification w Supports human plateable cryopreserved hepatocyte recovery and plating for induction assays or other assays requiring plated cells such as metabolism, transporter, and toxicity studies Tests on 25 lots of Corning Gentest inducible-qualified cryopreserved hepatocytes showed the Corning Gentest high viability cryohepatocyte recovery kit achieved ≥ 80% post-thaw viability for all lots. Recovery of viable cells also increased by an average of 1.5 million cells/vial (24% increase) when compared to other recovery and thawing medium. The non-cytotoxic nature of the recovery medium allows researchers to use a one-step centrifugation step and move directly to plating hepatocytes. The need for a second wash and centrifugation step is eliminated. Convenient format The Corning Gentest high viability cryohepatocyte recovery kit provides two tubes of post-thaw recovery medium and two tubes of plating medium for high throughput screening or pooled product applications. Figure 1. Viability Improvement versus Conventional Recovery Medium. Post-thaw viability increased 7% on average for a representative 10 Corning Gentest inducible cryopreserved hepatocyte lots when the Corning Gentest high viability cryohepatocyte recovery medium was compared to conventional recovery medium. Recovery Improvement Delta Recovery (M/vial) Single recovery centrifugation step saves time versus traditional two-step processes Delta Viability (%) Increases high post-thaw viability and yield Figure 2. Recovery Improvement versus Conventional Recovery Medium. Post-thaw recovery increased 1.7 million cells/vial on average (25% increase) for a representative 10 Corning Gentest inducible cryopreserved hepatocyte lots when the Corning Gentest high viability cryohepatocyte recovery medium was compared to conventional recovery medium.

Plating Efficiency and Cell Health Important Ordering Information HepatoLink® is the Corning immediate response system providing researchers with instant notification when Corning® Gentest™ hepatocyte products are available. For your convenience, select a preferred contact method— e-mail, phone, and/or SMS text alerts. Upon registration, you will be sent donor characterization data when a new lot becomes available. Register online at www.corning.com/lifesciences/hepatolink. Place your order directly through Corning’s online system. If you have any questions regarding Corning’s program or your...