Corning® Gentest™ Fresh Human Hepatocytes Hepatocytes in plates, sandwich culture, and suspension Corning offers a full portfolio of fresh hepatocytes in plates, sandwich culture, and suspension to support drug metabolism and transport studies. High-quality Corning Gentest fresh hepatocytes maintain morphology and function for long-term assays, such as enzyme induction, toxicity, metabolism of stable compounds, and drug transporter assays. Corning Gentest hepatocytes cultured on Collagen I with a Corning Matrigel® overlay in a sandwich culture form an in vivo-like hepatobiliary network that is critical for studying hepatic uptake and biliary efflux transporter activity. Compared to Collagen I surfaces alone, the Corning Matrigel overlay creates a more physiological-like environment, maintains hepatocyte-specific morphology, and improves basal and induced CYP450 activities. Supports ADME assays, such as metabolism, induction, transport, and toxicity requiring cultured human plateable hepatocytes Supports short-term ADME assays in suspension, such as metabolic stability and uptake transporter studies Saves researchers time with premade, ready-to-use hepatocyte cultures on Collagen I-coated plates with, or without, Corning Matrigel overlay Enables high throughput screening for discovery and development of drug candidates Corning Collagen I substratum with Corning Matrigel overlay – Improves cell health with increased basal and induced CYP450 Corning Matrigel Overlay Promotes a More In Vivo-like Environment The Corning Matrigel overlay improves basal activities for CYP450 activities compared to the conventional Collagen I coated surface. Corning Matrigel overlay improves both basal and induced CYP3A4 activity of human fresh hepatocytes (Corning fresh hepatocyte lot 323 cultured on Corning BioCoat Collagen I plates) – Supports bile canaliculi formation for efflux drug transport studies – Maintains typical hepatocyte morphology longer than a Collagen I Fresh hepatocytes can be cultured for more than one week, which makes them ideal for studies requiring longer culture times, such as induction, efflux transport, and toxicity assays. Multiple Formats Provide Researchers with Flexibility Corning’s portfolio of fresh hepatocytes includes flasks and 6, 12, 24, 48, and 96 well plates. These formats are available with Corning BioCoat™ Collagen I as a substratum with, or without, Corning Matrigel overlay. Fresh Hepatocytes Maintain Hepatic Morphology and Function CYP3A4 Activity in Corning Matrigel Overlay Sandwich Culture With Matrigel Overlay

Corning® Matrigel® Overlay Maintains Good Hepatocyte Morphology Corning Matrigel overlay maintains good hepatocyte morphology with 100% monolayer confluence in culture on Corning BioCoat™ Collagen I plates. Corning fresh hepatocyte lot 323 on day 5 on Corning BioCoat plates with Corning Matrigel overlay; Bright field (100x) Corning Fresh Hepatocytes in Sandwich Culture Enhance Extensive Bile Canaliculi Formation Corning fresh hepatocyte lot 323 forms bile canaliculi (Figure A) on day 5 culture in a Corning BioCoat Collagen I plate with Corning Matrigel overlay culture. Bile cana­ iculi formation...

Important Ordering Information HepatoLink® is the Corning immediate response system providing researchers with instant notification when Corning® Gentest™ hepatocyte products are available. For your convenience, select a preferred contact method—e-mail, phone, and/or SMS text alerts. Upon registration, you will be sent donor characterization data when a new lot becomes available. Register online at www.corning.com/lifesciences/hepatolink. Place your order directly through Corning’s online system. If you have any questions regarding Corning’s program or your participation, please contact us at...

Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not for use in humans. Not intended for use in diagnostic or therapeutic procedures. Corning Life Sciences makes no claims regarding the performance of these products for clinical or diagnostic applications. To place an order in the U.S., contact Customer Service at: tel: 800.492.1110, fax: 978.442.2476 email: [email protected] For technical assistance, contact Technical Support at: tel: 800.492.1110, fax: 978.442.2476 email: [email protected] Outside the U.S., contact your local distributor or...