Catalog excerpts
Corning® Gentest™ ATPase Assay Kit Colorimetric Reagent Kit for ABC Transporter Membrane ATPase Assays The Corning Gentest ATPase Assay Kit is a flexible, complete reagent kit delivering consistency across lots for testing all Corning human and animal ABC transporter membranes with one simple protocol. The assay kit complies with industry guidance recommendations. Features w Supports FDA-recommended P-gp and BCRP, as well as MRP membrane panels w Provides a simple, single protocol that accommodates all human and animal ABC transporter membrane studies w Delivers maximum flexibility allowing researchers the ability to perform a single transporter study or a full complement of P-gp, MRP, and BCRP studies with one kit w Saves researchers time by eliminating reagent preparation steps; assays can be completed within one day w Provides flexibility with single aliquot color reagents and stability for up to eight freeze-thaw cycles Kit supports industry guidance recommendations for ABC transporters The kit supports USFDA-recommended1 P-gp and ITC recommended BCRP2 in vitro studies. The kit includes verapamil and sulfasalazine to determine if a new chemical entity (NCE) is a substrate or inhibitor of P-gp or BCRP, respectively. Provides a high degree of reagent lot-to-lot consistency Three ATPase kit lots were evaluated for lot-to-lot consistency using the same hP-gp, hBCRP, and hMRP2 membrane lots and the results obtained demonstrated reproducibility within experimental uncertainty (see Figure 1/Table 1). Flexible format with one kit, one protocol for all human and animal transporter membranes Designed for maximum flexibility and researcher convenience, one ATPase protocol accommodates human and animal P-gp, MRP, and BCRP studies. Multiple transporter-use format supports single transporter assays such as five P-gp assays, or any combination of P-gp, MRP, and BCRP assays. Robust design supports multiple time usage Single reagent aliquots allow researchers to use one plate at a time (supports five 96 well plate assays), while saving the remaining reagents for use at a later date. The robust kit design supports up to eight freeze-thaw cycles with no significant loss in activity (see Figure 2). 1 USFDA/CDER, Drug Interaction Studies - Study Design, Data Analysis, and Implications for Dosing and Labeling, DRAFT GUIDANCE, September 2006. International Transporter Consortium (ITC) Drug Transporter Guidance Paper, Nature Reviews/Drug Discovery MARCH 2010 | VOLUME 9 (www.nature.com/reviews/drugdisc)
Open the catalog to page 1Corning® Gentest™ ATPase Assay Kit Ordering Information Kit contains reagents for five 96 well plate assays Cat. No. Assay Buffer 10 mM Phosphate Standard 50 mM ATP 10 mM Sodium Orthovanadate Assay Stop Solution Reducing Agent Color Solution A Color Solution B 1 mM Verapamil 1 M N-Ethylmaleimide 1 M GSH 100 mM Probenecid 25 mM Benzbromarone 1 mM Sulfasalazine Related Products Corning Gentest ABC Transporter Membranes Vanadate-Sensitive Substrate Stimulated ATPase Activity (nmol/mg/min) Human BCRP 10 µM PhlP Human MRP2 Human MRP3 1 mM 50 µM Probenecid Benzbromarone Figure 2. The Corning...
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