Corning Filtration Guide
20Pages

Corning® Filtration Guide Innovative Products for Filtration and Ultrafiltration

Contents Filtration Selecting the Best Filter for Your Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Improving Filter Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7...

F I LT R AT I O N Selecting the Best Filter for Your Application Choosing a filter does not have to be complicated – Corning has simplified the process. Just follow these four easy steps: Step 1: Match your application with the best pore size. Step 2: Select the best membrane and housing material for your application. Step 3: Select the correct membrane area to optimize flow rate and throughput. Step 4: Choose the best filter design for your application. Step 1: Match your application with the best pore size. The pore size is usually determined by your application or objective. Mycoplasma...

Cellulose acetate (CA) membranes have a very low binding affinity for most macromolecules and are especially recommended for applications requiring low protein binding, such as filtering culture media containing sera. However, both cellulose acetate and cellulose nitrate membranes are naturally hydrophobic and have small amounts (less than 1%) of non-toxic wetting agents added during manufacture to ensure proper wetting of the membrane. If desired, these agents can be easily removed prior to use by filtering a small amount of warm purified water through the membrane or filter unit....

Chemical Compatibility The mechanical strength, color, appearance, and dimensional stability of Corning® filters are affected to varying degrees by the chemicals with which they come into contact. Specific operating conditions, especially temperature and length of exposure, will also affect their chemical resistance. Table 3 provides a general guideline for the chemical resistance of Corning filter membranes and housings. Table 3. Chemical Resistance Guide for Corning Filters* Filter Membranes Chemical Class Housing Materials Weak Acids Strong Acids Aliphatic Amines Aromatic Amines Key: 1 =...

Step 4: Choose the best filter design for your application. Corning offers three basic filter types: positive pressure-driven syringe and disc filters, Spin-X® centrifuge tube filters driven by centrifugation, and vacuum-driven filters. The vacuum-driven filters offer several different designs and styles in disposable plastic products. Syringe/Disc Filters The smaller conventional Corning® syringe disc-type filters (4, 15, and 25, 26 and 28 mm diameter) are used with syringes which serves as both the fluid reservoir and the pressure source. They are 100% integrity tested. The HPLC-certified...

Improving Filter Performance Getting the best performance from your filtration products requires two very important steps: selecting the right products for the job, and then using these products effectively. The first part of this brochure covered the steps required to select the right filter for your applications; this section will help you optimize the filtration process by keying on the two most important areas – maximizing filter flow rate and throughput or capacity. The flow rate and throughput of filters are dependent on many variables. Some variables, such as temperature, pressure,...

Effect of Membrane Area The easiest and most practical way to increase filter flow rate is to increase the effective surface area of the filter membrane. Corning offers both syringe and vacuum filter units with a choice of membrane diameters that give a wide range of flow rates and throughputs (See Table 4). Effect of Fluid Temperature For most applications, filtering solutions at room temperature is fine. Usually increasing the temperature of a solution will increase the flow rate. For example, increasing the temperature of cell culture medium from 4°C to 37°C resulted in a doubling of the...

Effect of Prefiltration A simple way to improve filter performance is to pretreat your solution. High speed centrifugation will remove most suspended particles and reduce filter clogging, extending both flow rate and throughput (Corning® 250 and 500 mL centrifuge bottles are ideal for centrifuging larger liquid volumes). Prefiltration through a glass fiber pad or depth filter will also reduce particle load and premature membrane clogging. The use of a glass fiber prefilter has been demonstrated to more than double the throughput when filtering calf serum. These glass fiber prefilters are...

Ordering Information Syringe Filters ► A variety of membranes are available to meet your needs: polyethersulfone (PES) - low protein binding and faster flow rates; surfactant-free cellulose acetate (SFCA) - lowest protein binding; polytetrafluorethylene (PTFE) - chemical resistance; regenerated cellulose (RC) - best choice for DMSO compatibility; nylon (NY) - hydrophilic, surfactant- free and lowest extractable. ► 100% integrity tested, certified nonpyrogenic and noncytotoxic, manufactured in accordance Syringe Filters Ordering Information Diameter Size Membrane Housing Inlet/ Qty/ Cat. No....

150 mL Tube Top Vacuum Filters ► Minimizes unnecessary transfers by filtering directly into a 50 mL centrifuge tube ► Includes two centrifuge tube stands with each case ► Each polypropylene centrifuge tube is supplied with an individually wrapped cap for storage ► Individually packaged, sterile, certified nonpyrogenic 150 mL Tube Top Vacuum Filters Ordering Information Funnel Size/ Pore Size Cat. No. Membrane Tube Size (mL) (um) Qty/Cs Vacuum Filter Systems ► Filters feature printing on the funnel for easy product identification. ► Angled hose connector simplifies vacuum line attachment. ►...