CITSens Ion product info Ammonium-probe biotech
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Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:13.00pt "Arial", sans-serif; } .font2 { font:17.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS Product information & SOP for the Calibration of the Ammonium-probe Telefon: +41 (0)43 477 85 55 Fax: 0041 (0)43 477 85 57 Օ Mail: info@c-cit.ch Homepage: www.c-cit.ch

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:9.00pt "Arial", sans-serif; } .font2 { font:11.00pt "Arial", sans-serif; } CITSens Ion Ammonium probe Product information The CITSens Ion Ammonium probe from C-CIT AG can be used for continuous in-line monitoring of ammonium in cell cultures. The CITSens Ion Ammonium probe is especially designed for the use in bioreactors with animal/ human cells, plant cells and microbiological cell cultures. The CITSens Ion Ammonium probe (Art. No....

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:9.00pt "Arial", sans-serif; } The CITSens Ion Ammonium probe is especially recommended together with the CITSensI on 6-Chanal hightresolution Potentiometer(Art. Nr.: 3000-101) from C-CIT AG. In combination with the the CITSens Ion 6-chanal hight resolution Potentiometer you can measure up to 6 Ammonia-probes at the same time. The use of the CITSens Ion Ammonium probe in combination with a commercial ph-meter is due to technical reasons no...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:17.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS SOP for the Calibration of the Ammonium-probe in pure sait solution to control the functionality Telefon: +41 (0)43 477 85 55 Fax: 0041 (0)43 477 85 57 Օ Mail: info@c-cit.ch Homepage: www.c-cit.ch

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:9.00pt "Arial", sans-serif; } .font2 { font:10.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS 1. Calibrating the ammonium lectrode in-situ (in the bioreactor) Only build in and calibrate the sensor after heat strilisation. 1.1. Preparation of the sensor 驕 After heat sterilisation unpack the gamma sterilised ammonium electrode and insert it in to the bioreactor (PG 13.5 insertion) in a sterile environment. Connect the...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:4.00pt "Arial", sans-serif; } .font1 { font:8.00pt "Arial", sans-serif; } .font2 { font:9.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS 2. Checking the lectrodes performance outside the bioreactor (slope, detection limit) The following procdure describes how to calibrate the CITSens Ion Ammonium sensor in pure solutions to check the basic functionality of the electrode. If you intend to use the sensor in a sterile bioreactor don't use this procedure but follow...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:4.00pt "Arial", sans-serif; } .font1 { font:7.00pt "Arial", sans-serif; } .font2 { font:8.00pt "Arial", sans-serif; } .font3 { font:9.00pt "Arial", sans-serif; } .font4 { font:7.00pt "Times New Roman", serif; } INNOVATVE SENSORS - POWERFUL SOLUTIONS 2.2.1. Prϩparation of diluted solutions in the range of 102 to 10^ M ammonium (NH4+) (see picture below). The main concentrations of interest are 0.5 to 2.5 mmol/L ammonium ions or 5*10-4 to 2.5*10.-3 M L-1 NH4+. Solution 2 / 10-2 M:...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Arial", sans-serif; } .font1 { font:9.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS 2.3.Calibration procedure In order to measure the mV-signal referred to the ammonium concentration, the diluted solutions have to be transferred from the 100 ml flask to a bigger glass vessel. The ammonium-specific electrode and the reference electrode are immersed in each of the six solutions (solution 1 to 6). After 4-5 minutes the signal is collected over 30 s. The...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:7.00pt "Arial", sans-serif; } .font1 { font:8.00pt "Arial", sans-serif; } .font2 { font:9.00pt "Arial", sans-serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS Table 1: Example of an Excel table (an "excel file" is added to this SOP which supports the calibration process) specimen, solution Nr. concentration in mol L1 log c of concentrations Signal intensity in mV 1 10-1 -1.00 59 2 10"2 - 2.00 0 3 5*10-3 -2.602 -28 4 10-3 -3.00 -55 5 5*10-4 -3.301 -82 6 10-4 -4.00 -119 Telefon: +41...

Product catalog of CITSens Ion table.main {} tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:7.00pt "Arial", sans-serif; } .font1 { font:8.00pt "Arial", sans-serif; } .font2 { font:9.00pt "Arial", sans-serif; } .font3 { font:12.00pt "Times New Roman", serif; } INNOVATIVE SENSORS - POWERFUL SOLUTIONS 2.4.Graphical prsentation The results are presented graphically as shown in Fig. 1. EMF vs. log c 100 f(x) = 59.07x + 116.11 R2 = 0.99 Singal intensity Lineare Regression fur Singal intensity 50 0 S -50 -100 -150 -4.5 -4 -3.5 -3 -2.5 -2 -1.5 log c -1 -0.5 2.5.Interpretation of...