Biosensors for the Analysis of Multispecific Molecules Cooperativity / avidity, affinity and kinetic rates determination for potency profiling. In vitro half-life analysis of biphasic dissociation events. Efficient microfluidics for the resolution of a wide range of affinities. Automated, pre-configured assay setup and analysis options. Target surface modelling in a biosensor Ligand density, for example on a cell surface, affects the probability of cooperative binding and therefore the actual surface half-time of the analyte. Antigen density-dependent half-time Surfaceempty functionalization: Time for 50% antibody dissociation: 0 EGFR empty Contact [email protected] to speak to our application team about methodologies or to arrange a demonstration. average DISTANCE betweenbetween nanolevers: average DISTANCE 30 nanolevers: nm … >300 nm 50 nm ... >500 nm switchSENSE® variable chip surface modification allows imitation of in vivo target distribution by controlled adjustment of the distance and ratio of multiple ligands. switchSENSE® bispecific surface Half-time analysis of a bispecific antibody from surfaces with empty different ratios of EGFR and HER2 reveals potency of antibody HER2target cells. on differen

Cooperation of affinities leads to avidity Affinity maturation by second aptamer 108 The combination of two individual binding events results in an unpredictable cooperation (avidity) that must be measured to fully understand the interaction. switchSENSE® color-codes two ligands and thereby obtains both individual affinities and the combined avidity rates in one single automated assay. Made with www.affinity-avidity.com Two color-coded aptamer ligands immobilized on the chip surface. Rate scale plot of the association and dissociation rates of Thrombin to two Thrombin-binding aptamers. Individual...