PN-30
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Catalog excerpts

PN-30 - 1

ooLXJ Poster Note PN-30 Analysis of propolis extracts and isomeric flavonoid mixtures using trapped ion mobility spectrometry (TIMS)Bruker's new timsTOF instrument extends high performance QTOF mass spectrometry with high resolution ion mobility separation. Here, the novel platform is used for analysis of isomeric flavonoid mixtures as well as spiked and non-spiked propolis extracts. Authors J2S Sven W. Meyer; Peter Sander; Desmond Kaplan; Detlev Suckau Bruker Daltonics, Bremen, Germany Introduction Flavonoids are secondary metabolites produced by plants. Several subclasses of flavonoids are known, with a total number of published structures exceeding 8,000 compounds. Many of them show significant biological activity. Propolis is a natural bee resin that contains high levels of flavonoids, because the raw material stems from plants. Due to the enormous structural diversity of flavonoids, the analysis of complex samples, such as Propolis, is very challenging. Ion mobility separation coupled to high performance mass spectrometry (LC-IMS-MS/MS) introduces an additional analytical dimension for the identification of co-eluting isomers, based on the collisional cross section of the compounds. For very complex mixtures conventional ion mobility devices often do not have enough resolving power.

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Methods We used a high resolution QTOF mass spectrometer coupled to trapped ion mobility spectrometry (Bruker timsTOF TM) for analysis of propolis extracts and isomeric flavonoid mixtures. Propolis samples were collected from different bee yards across Germany (Fig. 1) and extracted with 70% EtOH using an ultrasonic bath. The supernatants were used as complex blank matrices. Propolis extracts and isomeric flavonoid mixtures were additionally spiked with mixtures of isomeric flavonoids at a concentration of 1 μg/mL (apigenin, galangin, quercetin, morin, orientin, isoorientin, quercitrin,...

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Results Several mixtures of isobaric flavonoids were investigated in direct infusion and LC-MS experiments complemented by high resolution ion mobility separation (Bruker timsTOF™), This workflow is particularly beneficial for co-eluting isobaric compounds and we analyzed compound mixtures consisting of galangin and apigenin (C15H10O5, Fig, 2A), orientin, isoorientin and quercitrine (C21H20O11, Fig, 2B), kaempferol, fisetin, scutellarein and luteolin (C15H10O6) as well as quercetin and morin (C15H10O7, Fig, 2C), High resolution ion mobility in the range of 150-250 often provided...

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timsTOFTM Flexibility to Empower Your Ideas For more Information visit For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen · Germany Phone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 ms.sales.bdal@bruker.com - www.bruker.com to change specifications without notice. © Bruker Daltonics 07-2016, PN-30, 1845961 Bruker Daltonics is continually improving its products and reserves the right

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