timsTOF • Powered by PASEF TIMS-QTOF MS Innovation with Integrity

The new standard for high speed, high sensitivity shotgun proteomics The timsTOF Pro with PASEF technology delivers revolutionary improvements in scan speed, coupled with enhanced specificity and high sensitivity. It does so while simultaneously maintaining ultra-high resolution for both precursors and MS/MS spectra. This unique performance quintet gives scientists the tools to dig deeper into the complex biology of the deep proteome. Novel dual TIMS design delivers near 100% duty cycle The novel design allows incoming ions to be accumulated in parallel in the first section and for ions to be...

PASEF digs deeper into the proteome PASEF delivers unmatched effective MS/MS acquisition rates and sensitivity by interfacing the time­ focusing power of the TIMS dimension with ultra-high resolution QTOF technology. Due to the s ­ ensitivity gains from PASEF it is possible to analyze samples with low cell populations and shorter , gradients can be used while identifying more peptides and their post-translational modifications (PTMs). Retention time Retention time Power of TIMS separation: (A) Shotgun proteomics data feature two dimensions at the MS level, the m/z and the retention time (MaxQuant...

Enabling record breaking speed with PASEF The timsTOF Pro was engineered by Bruker experts for scientists to enable breakthrough discoveries in shotgun proteomics. Using parallel accumulation, all ions that enter the timsTOF Pro can be analyzed. In addition, higher ion transmission is provided by the newly designed TIMS synchronized quadrupole. The high speed collision cell and ultra-high resolution TOF enables high MS and MS/MS spectra quality at high speeds. Optimized for shotgun proteomics UHR-TOF with high efficiency technology TIMS synchronized quadrupole mass filter High speed collision...

B PASEF MS/MS Heat Map Mobility TIMS MS Heat Map 1/K0 The principle of PASEF acquisition: Peptide ions are separated using trapped ion mobility spectrometry, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map (A). For acquisition of PASEF MS/MS spectra (B) the same TIMS separation is used with the quadrupole isolating a certain ion species only during its mobility elution time and immediately shifting to next precursor. Parent and fragment spectra are aligned by mobility values. Parallel accumulation serial fragmentation (PASEF) cycle time: By synchronizing the quadrupole...

timsTOF Pro for high sensitivity shotgun proteomics Recent advances in proteomic technologies have made MS-based proteomics a central research tool. However, truly complete proteomes are still elusive, mainly due to the high complexity and dynamic range of biological samples. Analyses using low sample amounts or where a range of post-translational modifications (PTMs) need to be located additionally remain challenging. With the timsTOF Pro, deep proteome coverage can be achieved without the need for large sample amounts ( > 1 µg), reducing the sample preparation costs and making it very attractive...

Higher throughput and more PTMs revealed using PASEF Due to the extremely high data acquisition speed provided by PASEF on the timsTOF Pro without a loss in sensitivity and resolution, typical proteomic analyses can be performed even faster, increasing the throughput of biological experiments tremendously. In addition, PTMs can be better separated from each other using TIMS resulting in a higher identification rate. Deep proteome coverage using short gradients A protein groups Deep proteome coverage with short gradients: Number of identified proteins groups (A) and unique peptides (B) using different...

Specificity Sensitivity Speed Prof. Dr. Jürgen Cox, Group Leader Computational Systems Biochemistry, Max-Planck-Institute of Biochemistry, Germany “ e have been impressed with the quality of the data that we`ve W seen and therefore we expect that the performance of the timsTOF Pro with the PASEF technology will make it very popular among proteomics researchers.” List of timsTOF patents TIMS cell (BIL 031/08), Title: “Apparatus and method for parallel flow ion mobility spectrometry combined with mass spectrometry”, Issued: US7838826B1; US 8288717B2 Parallel Accumulation TIMS (BRE 398/15), Title:...