nano-Advance UHPLC Enhancing The Power of Analytical Separations Innovation with Integrity LIQUID CHROMATOGRAPHY

The nano-Advance UHPLC System The nano-Advance UHPLC system delivers exceptional separations with a host of productivity enhancing features to provide the very best nano UHPLC system. Ideally suited as a key component of an LC-MS system, the nano-Advance UHPLC dramatically increases MS productivity and utilization while providing users with the utility and robustness needed for the most demanding applications. Making a Difference in LC-MS A Premium Separation System that Exceeds Performance Expectations An Exceptional Component of an LC-MS System for Protein and Peptide Analysis The nano-Advance...

Performance and Versatility To Enhance LC-MS Sample Carryover Determination The nano-Advance UHPLC system can be configured to single or multidimensional approaches and for UHPLC conditions to further enhance the chromatographic separation and provide insight into even the most complex protein or peptide mixtures. Integrating the versatile nano-Advance UHPLC into an LC-MS system is very easy with a wide range of choices available. The UHPLC system is compatible with Bruker‘s entire range of Ion Trap, orthogonal Time-Of-Flight and Ultra High Resolution Time-Of-Flight mass spectrometers. Control...

Bottom-Up Proteomics by LC-MALDI on nano-Advance and ultrafleXtreme Bruker‘s nano-Advance UHPLC system provides excellent chromatographic separation, enabling the analysis of even the most complex proteomic samples. Combined with the new ultrafleXtreme, Bruker‘s next generation MALDI TOF/ TOF, best results are guaranteed, allowing analysts to delve deeper into the proteome than ever before. Survey view of LC-MALDI results from a complex sample Highest performance by combining nano-Advance UHPLC separation with MALDI ultrafleXtreme. A zoom into the survey view details reveals the huge information...

The tryptic digest of 5 µg HeLa cell lysate yielded 41,551 well-resolved peptides after separation on the nano-Advance LC in a 3.5 h gradient. ProteinScape result viewer 5 μg HeLa Cell digest 30,268 precursor selected for MS/MS Bruker’s ProteinScape software provides a clear overview of data sets and entire projects with support for qualitative and quantitative work flows from in-depth analysis to publication-compliant reporting. From sequence coverage of individual proteins to chromatographic distribution on peptide level and MS/MS-spectra identification - all information is available at-a-glance....

Glycoprotein Characterization by LC-MALDI on the nano-Advance The analysis of protein glycosylation, e.g., in Biopharmaceuticals is challenging due to the heterogeneous nature of this posttranslational modification. LC-MALDIMS/MS using the nano-Advance UHPLC and the new ultrafleXtreme MALDI-TOF/ TOF directly provides access to determining N- and O-linked glycosylation sites and glycan structures. The GlycoQuest search engine in Bruker’s ProteinScape package offers an industry’s first for automated glycan analysis. Glycopeptides are automatically detected in LC-MALDI datasets and identified with...

Highly reproducible retention times GlyoQuest viewer with glycan result reporting iiH-u na.ip jug rntn nn ProteinScape view after a GlycoQuest search. Glycan search results are displayed in a Glycan and a fragment table. In addition, the proposed glycan structures and the annotated MS/MS spectra are displayed. Intensity " Repetition of 18 LC-runs High retention time stability at nanoflow rates. Retention times were followed format 18 LC-runs of an E. coli digest (300 ng] in a 120min gradient. Excellent relative standard deviations for retention time were achieved throughout the complete gradient...

nano-Advance UHPLC System Specifications Physical Characteristics: Size: Depth: 21.0 in (54 cm) Width: 13.0 in (33 cm) Height: 12.5 in (32 cm) Weight: 50.2 Lbs (23kg) Biocompatible Fluid Path: 10,000 PSI / 700 bar Biocompatible Sample Needle Coating Injection Modes: Full Loop or Zero Loss Partial Loop Injection Volumes: 1 - 1000 μl Loop/Trap Injection Syringe Sizes: 25, 125 μl Sample Capacity: 2x 54 Vial Racks, 96 or 384 Well Plates) Temperature Control Option: 4°C - 40°C (Sample Temperature) Gradient Flow Range: 50 nl to 5,000 nl/min in 1 nl increments System Pressure Range: 0 – 10,000 PSI (70...