Bruker Daltonics micrOTOF-Q II Cutting Edge Performance with Sub-ppm Confidence think forward ESI-Qq-TOF

The advantage of Ultimate Confidence Do you need maximum confidence in your analytical system? Bruker Daltonics’ new micrOTOF-Q II™ ESI-Qq-TOF mass spectrometer features the very latest technology developments to provide maximum certainty in your research in Small Molecule Identification, Metabolomics or Proteomics. SmartFormula determination # possible formulae Mass Accuracy several ppm accuracy plus Chemical Expertise additional confidence numerous medium plus SmartFormula (Mass Accuracy+TIP) sub-ppm confidence few plus SmartFormula3D (fragments-based TIP) unambiguous formula ID single Three...

Unique Power3 – Simultaneously without Compromise Cutting-edge performance has never been this easy to use: Sensitivity Experience market leading sensitivity with the next-generation Apollo II™ ion funnel ESI source, dramatically improving ion transmission. Mass accuracy Be impressed by a mass accuracy of 1 – 2 ppm over an exceptionally wide dynamic range. Resolution Be amazed by superb focus resolving power exceeding a resolution of 17,500 – 20,000 FWHM. Fragment analysis Featuring a superb quadrupole mass filter and a quadrupole collision cell for accumulation of parent and fragment ions prior...

Unprecedented Certainty Isotopic pattern analysis measured spectrum calculated spectrum Precision in organic chemistry Accurate mass and Bruker Daltonics' proprietary SmartFormula 3D True Isotopic Pattern analysis algorithm provide unrivalled certainty for the Due to an extraordinarily wide dynamic range, the entire mass range is covered with a high mass accuracy - resulting in unambiguous molecular formula SCID-MS3 analysis of Pd-Complex in synthetic chemistry. A fragment m/z 157.0758 reveals a mass error of < 5 ppm (externa calibration) for reading out a formula by SmartFormula scoring Parent...

• Accurate Mass, SmartFormula and MS/MS Metabolic fragment ID and statistical analysis Advancing metabolomics research Metabolic profiling promotes biomarker discovery to the metabolome level to address profiles that are powerful sen- sors reflecting situations at physiological Identify small molecule biomarkers with the power of accurate mass, TIP and fragments analysis for evaluation of disease state, following drug treatment or to monitor toxic response. Tailored tools for pattern recognition, metabolite MS and MS/MS identification complete the solution package for metabolome research. @ 0MS...

Information-Rich in Depth Analysis Mass Distance The de novo sequencing machine instrument to achieve outstanding mass accuracy over a broad mass range, a prerequisite for successful de novo sequencing - from low-mass immonium ions to high-mass fragment ions. MS/MS spectrum of GluFib m/z = 785.8 Exceptional mass accuracy leads to outstanding de novo sequencing results Mass accuracy Matched peptides shown in Bold Red Powerful identifications over a wide dynamic range High mass accuracy is available on both the peptides and fragments levels. Impressive mass accuracy on all meets unrivalled mass...

• Proteomics Applications without Compromise Accurate mass measurements of PTMs Sulfation? Phosphorylation? Understanding proteomes requires knowledge about posttranslational modifications. Detailed insight into the molecule's structure is achieved with the excellent mass accuracy of the micrOTOF-G II - even with minor mass Count on the ability to quantitate Stable isotopic labeling using iTRAQ™ or the newly invented technology ICPL™ allow fast and convenient, yet easy-to- use, quantitative proteomics research. Excellent sensitivity is achieved due to the technically advanced ion funnel ESI source...

Sensitivity, Dynamic Range & Resolution MS/MS ID from 100 amol BSA Intens. 4 x10 0 MS, EIC of expected peptides +MS2(740.77), 53.2-53.3min #3862-#3865 1500 1.5 500 1.0 740.91 300.16 1000 590.83 685.45 387.27 500.36 175.12 1017.60 813.51 1180.67 0 0.5 0.0 30 35 40 45 50 55 60 Time [min] Analysis of 100 amol BSA digest on column. BSA was unequivocally identified by five peptides. Left: Extracted Ion Chromatograms of identified peptides. Sensitivity More than 5 orders dynamic range Ultimate sensitivity is demonstrated with a 100 amol digested BSA sample. Even for this small sample amount, solid...

Ultimate Performance with Superior Detection Power Fragments analysis ISCID Q-Separation Optional: ISCID + Standard: Fragment analysis The micrOTOF-Q II provides exceptional mass accuracy in MS/MS for metabolite and protein identification. Achieve optional additional fragmentation performance with a source designed for ion fragmentation prior to the quadrupole (In-Source CID). After isolation in the analytical quadrupole, the ISCID fragments can be further fragmented in the collision cell (CID). MS3 with the micrOTOF-Q II Reserpine: In-Source CID (left), Isolation of ISCID ions and fragmentation:...

Outstanding Solutions Target screening Multitarget Pesticide Screening: hr EIC of detected compounds with ± 0.002 Da mass window. qualitative multi-target screening -forensics, doping control and residue analysis The TargetAnalysis™ application allows multi-target compound screening in com- plex matrices, providing high specificity and enhanced SmartFormula™ molecular formula determination. Multi-target Screening Due to a full-scan accurate mass approach, the solution is able to screen thousands of compounds from a single sample LC/ESI-TOF run and enables screening for large compound libraries...

Empowering Accessories Metabolite ID user interface Chemical formula generation Compass OpenAccess™ provides an automated walk-up LC/MS system for chemical formula generation, molecular formula confirmation and generic LC/MS measurements. This client-server based software supports LC/MS workflows especially for chemists in laboratories with various levels of instrumental analysis experience. Metabolic Profiler™ Advance biomarker profiling to the metabolome level to address metabolomic profiles that are powerful sensors reflecting situations at physiological endpoints. An application feasible...