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CaptiveSpray

CaptiveSpray

CaptiveSpray

Product catalog summary
Introduction
The document introduces the CaptiveSpray, a revolutionary LC-MS source developed by Bruker Daltonics. It combines the sensitivity of Nanospray with the ease of use of Electrospray, designed to enhance performance and reliability in nano flow mass spectrometry (MS).
Key Features
  • NanoSpray Sensitivity: Operates effectively at 50-5000 nL/min LC flow rates.
  • Ease of Use: Plug and play operation simplifies usage.
  • Robust Design: Minimizes clogging and ensures superb reproducibility, especially for protein quantitation.
  • Compatibility: Works with Bruker, AB Sciex, and Thermo LC-MS instruments.
Technical Specifications
The CaptiveSpray source utilizes patented technology to capture and sweep sample ions into the MS, independent of the LC flow rate. It is compatible with all current Bruker ESI-MS systems and can be used at low µL flow rates, allowing for larger ID columns and improved dynamic range for low abundance protein identification.
Performance Comparison
  • Bruker MS: Identified 1307 proteins from a 1ug E. coli digest.
  • Thermo MS: At 200 nL/min, identified 467 proteins with Nanospray; at 600 nL/min, identified 881 proteins with CaptiveSpray.
  • AB Sciex MS: Demonstrated excellent reproducibility over 960 runs of 20 fmol BSA+BGL in 1ug E. coli on an API4000.
Design and Functionality
The CaptiveSpray source connects an LC column through a capillary spray tip directly to the MS inlet. A vacuum seal creates an air funnel that desolvates sample ions, allowing faster LC flows and higher sample loading. The patented “Etch-Taper™” spray tip resists plugging and fouling, enhancing durability and performance.
Conclusion
The CaptiveSpray source is ideally suited for quantitative proteomics and biomarker discovery, offering increased sensitivity, faster run times, and improved dynamic range. It is designed for research use only and not for diagnostic procedures.
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Catalog excerpts

CaptiveSpray-1

CaptiveSpray The Revolutionary LC-MS Source Innovation with Integrity LC-MS

 Open the catalog to page 1
CaptiveSpray-2

■ NanoSpray Sensitivity at 50-5000 nL/min LC Flow Rates ■ Easy to Use Plug and Play Operation. ■ Robust Design Minimizes Clogging ■ Superb Reproducibility Especially for Protein Quantitation ■ Higher Sample Loading Capacity Delivers Increased Dynamic Range ■ Compatible with Bruker, AB Sciex and Thermo LC-MS Instruments LC-MS source that combines the sensitivity of Nanospray with the ease of use of Electrospray. CaptiveSpray utilizes patented technology to capture and sweep sample ions into the MS- inde- pendent of the LC flow rate. The source fits all current Bruker ESI-MS systems. flow rates...

 Open the catalog to page 2
CaptiveSpray-4

How CaptiveSpray Works The CaptiveSpray source couples an LC column through a capillary spray tip directly to the inlet of the MS. A vacuum seal is then created and air is pulled in and around the spray tip creating an air funnel that sweeps the spray tip and desolvates the sample ions as they are pulled into the MS. This design allows faster LC flows and higher sample loading, resulting in increased sensitivity and/or significantly shorter run times. The patented “Etch-Taper™” spray tip is not susceptible to the plugging, fouling or degradation that effects most internally tapered NanoSpray...

 Open the catalog to page 4

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.