CaptiveSpray nanoBooster™ The Revolution in Proteomics Ionization Innovation with Integrity LC-MS Source

The Key to Performance and Reliability for nano flow MS Bruker CaptiveSpray principle: Stable and robust nanoflow LC/MS is still a challenge in proteomics analysis. The Bruker CaptiveSpray source is a revolutionary ion source with a patented design that provides provides easy operation just as simple normalflow electrospray. CaptiveSpray delivers nanospray sensitivity, resists plugging, and provides reproducible uninterrupted flow for even the most complex proteomics samples. Bruker‘s revolutionary CaptiveSpray source is now available with smart dopant addition option – the nanoBooster CaptiveSpray...

Reliability and Robustness Ease of use CaptiveSpray is providing nanospray sensitivity with the ease of use and robustness of ESI. The patented Etch Taper™ technology provides a spray tip that is not internally tapered like the tip of a typical pulled nanospray ionization needle. This results in significantly less clogging of the spray tip allowing more samples to be analyzed with greater confidence. CaptiveSpray provides a vortex gas that sweeps around the emitter spray tip to desolvate and to focus the Taylor cone into the MS inlet capillary. The vacuum seal to the MS ion guide draws all of...

without nanoBooster Boost Your ID Rates CaptiveSpray nanoBooster increases the ionization efficiency of peptides significantly, leading to a much higher sensitivity than even conventional nanospray. This results in a highly improved number of identified proteins in complex mixtures. CaptiveSpray nanoBooster CaptiveSpray nanoBooster without CaptiveSpray nanoBooster Hela cell digest,100 ng lysate Significant increase in signal intensities for low sample amounts. Charge enhancement for Proteomics A without nanoBooster Number of identified proteins +14% without nanoBooster without nanoBooster Data...

Plug - and - Play The CaptiveSpray source mounts directly onto all current Bruker API mass spectrometers. The Ion Transfer Interface connects directly to the front of the capillary, eliminating the need for complex XYZ adjustable stages. Flexibility The Bruker CaptiveSpray operates at a wider flow rate range than traditional nanospray. CaptiveSpray delivers stable and reproducible gradient spray from 50 nL/min to 5 uL/min allowing the use of a wide range of column diameters to deliver optimal performance for a dynamic range of experiments. Comparison of charge envelop for an intact mAB The gas...

CaptiveSpray nanoBooster sheath gas with acetonitrile results glycopeptides. The charge state increase provides higher quality ETD spectra and enables highly improved glycopeptide identification of the glycosylation sites. CaptiveSpray nanoBooster allows the abundant glycopeptides and their glycan structures due to higher quality MS/MS ETD reveals full information on glyco- peptides: glycan structure, sequence Structure elucidation by combination of CID amd ETD Follicle-stimulating hormone alpha chain: hCGa [M + 4H]4+=767.47 m/z; Top: (CID) Identification of A/-glycan struc- ture with GlycoQuest....

... enables glycopeptides analysis Ionization and charge effects on /V-glycopeptides Charge distribution and intensities of A/-glycopeptides measured on amaZon speed ETD with CaptiveSpray and nanoBooster. Bruker - The Proteomics Company nanoBooster perfectly fits to all Bruker ESI MS systems with the common APOLLO II ionization frontend. Together with the Bruker nano-Advance UHPLC you get the perfect nano to cap flow LCMS system for proteomics appli- A common platform - out of one hand.

CaptiveSpray nanoBooster™ The ability to use larger columns with higher loading capacities provides higher sensitivity and dynamic range than conventional nanospray. Dynamic range of the CaptiveSpray ionization source The stable and reproducible nature of the spray makes the CaptiveSpray ideal for quantitative proteomics • • • • • • Increased nanospray sensitivity at 50-5000 nL/min LC flow rates Smart dopant enrichment of ionization gas for charge state control Easy to use plug-and-play operation Robust design minimizes clogging Superb reproducibility especially for protein quantitation Higher...