Technical_Note_Cooling_Blocks
5Pages

TECHNICAL NOTE PRODUCT INFORMATION BRAND Cooling Block and Liquid Handling Station BRAND Cooling Blocks Passive cooling of liquids in PCR and micro tubes in the BRAND Liquid Handling Station Overview Whether in a small or large laboratory, or for manual or automatic liquid handling – the cooling of liquids is essential for many biological applications. In this Technical Note, the cooling capacity of the BRAND Cooling Blocks passive cooling system is demonstrated using the 1.5 ml and 96-well versions as examples. The pipetteability of the cooled liquids is also examined. This allows liquids to be stored cooled for up to 3 hours regardless of their well position and pipetted in the Liquid Handling Station. With the BRAND Cooling Blocks, BRAND offers a viable alternative to expensive, high-maintenance active cooling systems. Introduction The cooling of liquids is essential for numerous biological applications – for example, to protect valuable samples from microbial degradation or to prevent the loss of activity of biologically active molecules. In automation, active cooling systems are often used to keep samples below 7 °C over longer periods of time. BRAND now offers the BRAND Cooling Blocks passive cooling system for 0.5 ml (709510), 1.5 ml (709511), and 5 ml micro tubes (709512) as well as Cooling Blocks in 96- and 384-well PCR format (709513 and 709514) for automation. In this Technical Note, the cooling performance of the cooling blocks is shown using the 1.5 ml micro tube and 96-well PCR variants as examples. It is also shown how long liquids stored in BRAND Cooling Blocks can be pipetted in the Liquid Handling Station in a cooled stat

TECHNICAL NOTE Material and methods Description Item number Deionized water 98 % ethanol Serum consisting of 1 % albumin and 0.001 % Triton X consisting of: Carl Roth Albumin fraction > 98 % powder bovine M = 69.000 Triton X 100 extra pure Liquid Handling Station Transferpettor, digital type, 10 – 50 µl Transferpette S-8, variable type, 20 – 200 µl Transferpette S, variable type, 2 – 20 µl Micro tube with lid lock, 1.5 ml The BRAND Cooling Blocks are frozen upside down in the freezer for 12 hours at approx. -20 °C to -23 °C as described in the operating manual. The media used (deionized...

TECHNICAL NOTE Results and discussion Cooling capacity of the BRAND 96-well PCR Cooling Block Cooling capacity of the BRAND 1.5 ml tube Cooling Block BRAND 1,5 ml Reaktionsgfäße Cooling Blocks Deionized water Pipetting time until 7 °C reached Pipetting time until 7 °C reached Waiting time until working temperature > 4 °C or not frozen Waiting time until working temperature > 4 °C or not frozen Figure 1 Cooling capacity of the BRAND 96-well PCR Cooling Block when using the BRAND 96-well PCR plate (781368) and different media (200 µl). Figure 2 Cooling capacity of the BRAND 1.5 ml tube...

TECHNICAL NOTE In general, it has been shown that the passive cooling system BRAND Cooling Blocks can keep the liquids in micro tubes or 96-well PCR plates below 7 °C for far longer than two hours (Figure 1 and Figure 2, page 3). (e.g. 40 % glycerol solution and ethanol) can be stored and pipetted cooled below 7 °C up to 160 min in 1.5 ml micro tubes and up to 180 min in 96-well PCR plates; waiting times are not necessary (Table 1 and Table 2, page 3). When using the Cooling Blocks to cool liquids with a comparatively high freezing point (e.g. deionized water or aqueous liquids with no or...

BRAND®, BRAND. For lab. For life.® as well as the BRAND figurative mark are registered trademarks or trademarks of BRAND GMBH + CO KG, Germany. All other trademarks mentioned or depicted here are the property of the respective owners. Our technical literature is intended to inform and advise our customers. However, the validity of general empirical values, and of results obtained under test conditions, for specific applications depends on many factors beyond our control. Please appreciate, therefore, that no claims can be derived from our advice. The user is responsible for checking the...