BlueSens Report No.3
19Pages

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Catalog excerpts

BlueSens Report No.3 - 2

Understanding Bioprocesses. BlueSens Report No. 3, January 2014 © 2014 by BlueSens gas sensors GmbH, Herten, Germany, www.BlueSens.com Page layout, artwork and cover design: Marcus Riepe, ebene1 Kommunikation GmbH, Düsseldorf, www.ebene1-kommunikation.de We are pleased to present the third BlueSens Report. Nearly two years after Report No.2, we can introduce to you the new report with interesting application reports about our gas sensors for bioprocesses. We always receive very good feedback for the BlueSens Report from our customers and prospects, and have now brought together many...

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BlueSens Report No.3 - 3

understanding bioprocesses 3 BlueSens. Introduction - The BlueSens Report No. 3 Application Reports 6 A Real-time Adaptive Oxygen Transfer Rate Estimator for Metabolism Tracking in Escherichia coli cultures By Matthew Pepper, Li Wang, Ajay Padmakumar, Timothy C. Burg, Sarah W. Harcum, Richard E. Groff 12 Assessing biodegradation of oil with marine bacteria by monitoring O and CO concentrations 2 online in a closed loop Fr A.G. Valladares Juárez, N. M. Enwena, M. Schedler, R. Müller Institute of Technical Biocatalysis, Hamburg University of Technology, Germany 17 Bio-hydrogen production by...

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BlueSens Report No.3 - 4

Application Report A Real-time Adaptive Oxygen Transfer Rate Estimator for Metabolism Tracking in Escherichia coli cultures By Matthew Pepper, Li Wang, Ajay Padmakumar, Timothy C. Burg, Sarah W. Harcum, Richard E. Groff, Department of Electrical and Computer Engineering/Department of Bioengineering, Clemson University. where Mf is the mass flow rate. The concentration b2 is sampled by the BlueInOne sensor, and the sensor output, b3, is modeled as a first order linear system with time constant 2, Estimator Mathematics The adaptive estimator is based on a structure from [6]. The mathematics...

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BlueSens Report No.3 - 5

Application Report Table 1 contains all the parameters for the oxygen dynamics model. kLa is known to slowly change over the course of a fermentation and is influenced by multiple factors such as anti-foam addition and liquid viscosity changes; therefore, the values for the linear model seen in Eqn. (2) are time-varying and can only be defined approximately. Parameter Mass Flow rate Culture volume Headspace volume BlueSens time constant Oxygen density Initial value Initial value Input Oxygen Volume Maximum Oxygen carrying capacity Initial Stir speed Experiments Bioreactor System The...

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BlueSens Report No.3 - 6

Application Report Bioreactor Conditions Two experiments were performed to test the accuracy of the OTR estimator. In the first experiment, the OTR estimator was implemented on a bioreactor simulator. The delays on input and output data characteristics mirror that of the Sartorius Biostat-B, the BlueInOne sensor, and the two balances. The simulated behavior of the Escherichia coli (E. coli) is based on the work of 1999 Xu [8]. In the second experiment, E. coli MG1655 pTV1GFP [5] was cultured for 12 hours in minimal media. The bioreactor was inoculated at an optical density (measured by...

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BlueSens Report No.3 - 7

Application Report Assessing biodegradation of oil with marine bacteria by monitoring O2 and CO2 concentrations online in a closed loop Because of the hydrophobic nature of the oil, at least­ two immiscible phases appear when mixed with seawater and bacteria, making it impossible to sample the system representatively. Moreover the low molecular weight components of the oil are volatized during sampling and that affects the experiment. Currently, we have been quantifying the degradation of oil indirectly by offline analysis of bacterial concentrations or surement of oxygen or carbon dioxide...

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BlueSens Report No.3 - 8

Application Report Application Report Figure 4: CO2 ( ), O2 ( ) and bacterial ( ) concentration of the i ­ncubation of strain DWHO6A in minimal medium with 0.1% (v/v) oil Figure 8: CO2 ( ), O2 ( ) and bacterial ( ) concentration of the i ­ncubation of strain DWHO6B in minimal medium with 1 mM toluene Figure 5: CO2 ( ), O2 ( ) and bacterial ( ) concentration of the i ­ncubation of strain GM2 in minimal medium with 1 mM hexadecane Figure 7: CO2 ( ), O2 ( ) and bacterial ( ) concentration of the i ­ncubation of strain DS4P5 in minimal medium with 0.1% (v/v) Louisiana oil Figure 9: CO2 ( ), O2...

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BlueSens Report No.3 - 9

Application Report Conclusion Spilled oil can be degraded by marine bacteria. Oxygen is a key substrate for aerobic microbial growth and biodegradation. Carbon dioxide and biomass are the major products of the biodegradation and can be used to estimate the amount of oil that has been degraded. The degradation capabilities of some deep-sea and surface-isolated bacteria could be tested and some promising strains were found. Sampling in this multiphase system was impossible and this was circumvented by the measurements with the BlueInOne analyzer. This accelerated the screening of the new...

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BlueSens Report No.3 - 10

Application Report and environmentally viable possibility, especially because, over the last several years, the demand and production of biodiesel has remarkably increased [1]. Methods ans materials Growth media, cultivation and experimental set up Different anaerobic and facultatively anaerobic bacteria were used for hydrogen production measurements from Microbial Strain Collection of Latvia (MSCL) - Clostridium sporogenes MSCL 764, Enterobacter asburiae MSCL 839, Enterobacter cloacae MSCL 778, Enterobacter aerogenes 758 MSCL. Experiments were continued with the best hydrogen producer...

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BlueSens Report No.3 - 11

Application Report Application of BlueSens® H2 sensor in a gas separation study P. Bakonyi, N. Nemestóthy, K. Bélafi-Bakó Research Institute on Bioengineering, Membrane Technology and Energetics University of Pannonia, Egyetem street 10, Veszprém, Hungary Introduction Moreover, evaluating the influences of notable process Biohydrogen is an emerging alternative energy carrier variables, namely separation temperature and recovery with high potential for sustainable development. It can factor was also aimed. be utilized in fuel cells or internal combustion engines. Materials and Methods...

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All BlueSens gas sensor GmbH catalogs and technical brochures

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Archived catalogs

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  2. REPORT 1

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