from laboratory scale to manufacturing process BIOREACTORS PILOT AND INDUSTRIAL SCALE

Pilot scale bioreactors CONTENTS

Pilot scale bioreactors INTRODUCTION We are an innovative company designing and manufacturing laboratory and pilot scale bioreactors. Our bioreactors meet up – to – date requirements and includes innovative solution. Model based control of fed - batch fermentation, as well as the use of novel of magnetic drive are as an example. We test our new bioreactor solutions in our laboratory through real fermentation processes. Our bioreactors have been designed in such a way to be easily supplemented and adapted to various applications. We manufacture laboratory bioreactors for cultivation of bacteria,...

Pilot scale bioreactors PILOT SCALE BIOREACTORS We offer stainless steel bioreactors from 10 till 20 000 litres, as well as bioreactor lines, including other technological vessels. An autonomous bioreactor consists of 3 main parts: • Bioreactor vessel with mixer; Technological stand; Control cabinet, including PLC.

Pilot scale bioreactors 30 litre bioreactor integrated in product line Screenshot of bioreactor control panel

Pilot scale bioreactors TECHNICAL SPECIFICATION Vessel design, volume Vessel design Jacketed with heat insulation. Removable lid (till 800 liters). The lifting device of lid. The vessels over 800 liters have manhole of corresponding size. Side glass on the lid and in the upper cylindrical part of the vessel. Surface quality Inner vessel wall: surface roughness lower than 0.63μm. Outer vessel wall: surface roughness lower than 0.8 μm. (pickled & passivated) • The material of the parts, contacting with product is stainless steel 316L; • For parts, not contacting with product (for example, jacket)...

Pilot scale bioreactors • Mass flow controller (MFC) in the range of 0 - 1 vvm (as an option); • Chemically inert PTFE membrane filter cartridges which are inherently hydrophobic used as air and gas-sterilizing filters for incoming and outgoing air (Manufactured by PALL, USA). Filters are installed in the steam sterilizable stainless steel housings; • Automatic gas flow control system (O2, N2, CO2, NH3) using a mass flow controller (the MFC) (optional); • The measurement of the O2 / CO2 concentration ratio in the exhaust air (optional); • Automatic pressure control system in the fermenter vessel,...

AUTOMATED CIP EQUIPMENT We offer two types of CIP equipment: • This system is mobile equipment, which is placed on movable platform. A such equipment can easy to move in production unit and to connect it to CIP washed fermenter or another technological vessels. Maximum speed of detergent circulation – 5000 l/h (it can be varied). 2 centrifugal pumps (for CIP circulation and discharge), 2 dosage pumps with side walls to concentrate detergents and other fittings, control board, valves for manual and automatic control, couplings and armature to provide the operation of CIP washer are placed on the...

Automated CIP equipment The given installation of CIP allows realized both manual and completely automatic modes of washing machine work process. For automatic control CIP washing mode should be adjusted or selected. These operations can be implemented by the control board. CIP washing mode consist of stages, but each stage – of steps. In fact stage of washing process is considered as realization of definite type of washing process with selected parameters (temperature, time of CIP washing, time of discharge output, detergent conductivity, speed of concentrate supply, fast speed of mixer rotation,...

Stationary CIP equipment is customized depending of the requirements of concrete production. This equipment is foreseen for CIP/SIP washing of bioreactors and another technological vessel, as well for the pipeline connections of technological line. It can be carried out CIP/SIP customized systems with different amount of CIP vessels. If is used one CIP vessel system, then in this vessel during defined phase is applied corresponding media and before next phase it is discharged. There are two working regimes of stationary CIP/SIP equipment: washing and sterilization. The regimes differ with settings,...

Automated CIP equipment The connections of steam line, cooling water, compressed air and discharge can be provided according following diagrams (variants with one and four vessels).

Automated CIP equipment CIP / SIP allows to carry out processes in two variants: 1) 2) Locally by control directly from operator panel of CIP / SIP; Centrally by control from remote operator panel or SCADA. The process of CIP / SIP are fully automatic, and in the case of necessity concrete device or process can be controlled manually. The automatic process of CIP is carried out according selected recipe. The recipe includes the stages, the steps with corresponding parameters. Any phase of washing consists of definite sequential steps. According to selected mode of washing process, steps can differ....

Industrial application examples of bioreactor lines INDUSTRIAL APPLICATION EXAMPLES OF BIOREACTOR LINES We have experience in application of complex bioreactor lines for biotechnological production. We develop technical requirements, project and qualification documents, manufacture equipment and provide the delivery / montage / starting up. Our experience covers the design of following application plants: 1. Substances for remediation of soil from oil pollution 2. Ferments for dairy industry 3. Vaccines for animals 4. Probiotics from whey lactose 5. Bio-fertilizers 6. Pichia pastoris fermentation...

Industrial application examples of bioreactor lines Substances for remediation of soil from oil pollution The application of this fermentation system is remediation of soil, contaminated with oil over a long period. In this system bacteria consortium have been cultivated, which has been isolated from oil-contaminated soil. Fermentation system consists of 30, 100 and 800 liter bioreactors. Another 800 liter reactor is used for storage of a substrate. In every bioreactor is temperature, pH, pO2, foam and level control, as well as time profiles of automatic substrate feeding rate can be adjusted....