Sample Preparation Workflow Solutions from Biotage

Homogenization and Liquid Solid Extraction Analyte Extraction and Extract Clean Up Processing Options Evaporation Highlighted Application: Acrylamide Highlighted Application: Mycotoxins Highlighted Application: Pesticide Analysis Highlighted Application: Extract Clean Up Ordering Information Vacuum Positive Pressure Food Analysis - Sample Preparation Workflow | © Biotage 2022

Food Analysis - Sample Preparation Workflow Applications Sample Preparation Workflows for Food Analysis Applications Typical Workflow Raw Sample Food and Agriculture sample preparation is often complicated by the nature of the sample matrix. Solid or semi-solid samples such as fruit, vegetables, tissue or soil need specialized sample pre-treatment for efficient extraction of the analytes. The complexity of these sample matrices requires robust and reliable methodology. Learn more about our smart sample preparation solutions for simplifying sample handling and clean-up of these difficult samples....

Homogenization and Liquid Solid Extraction Homogenization is used to ensure that all constituents in food sample matrices are fully homogenized, so the final result represents the entire sample. After homogenization, target analytes are then extracted from the solid matrix into a solvent via liquid solid extraction. It is important to have a fully homogenized sample in order to obtain and effective extraction of the target analytes. Some samples may only require a simple whizz in a food processor, but for other food matrices Biotage Lysera is a bead homogenizer capable of preparing representative...

A Range of Tubes and Beads Support Homogenization of Any Sample TypeLyse/Disrupt Homogenize Grind Size

Analyte Extraction and Clean Up Analyte Extraction and Clean Up Targeted extraction of your analytes, and extract clean up to ensure that matrix interferences are removed and your analytical system is not compromised, require quality extraction consumables for high analyte recovery and reproducible performance. Solid phase extraction (SPE) is a very powerful sample preparation technique for extraction of analytes from complex matrices. Biotage ISOLUTE and EVOLUTE solid phase extraction columns and plates are available in a comprehensive range of media and sizes to match your application and throughput...

Analyte Extraction and Clean Up Matrix Scavenging SPE Alternatively, the SPE columns can also be used in ‘matrix (or interference) scavenging, mode, where the sample is applied to the column and collected as it passes through. Interferences are trapped on the column, and do not enter the final extract. Step 1 Condition Step 1 Condition Step 2 Load Step 4 Wash Step 3 Elute Step 5 Elute Interferences Analyte of interest The matrix (interference) scavenging SPE process. QuEChERS Extraction and Clean-up The Quechers (Quick, Easy, Cheap, Effective, Rugged and Safe) technique combines initial salt...

Processing Options Processing Options Processing samples couldn’t be easier. Biotage offer vacuum and positive pressure based systems. Vacuum systems are well suited for processing larger sample volumes, whereas positive pressure processing can provide better flow control and consistency for lower volumes. Biotage VacMaster -96 manifolds are ideal for high throughput parallel processing of scaled down methods in 96-well plate format TM Process 96 samples in parallel SPE and filtration plate formats Manual Vacuum Manifolds Biotage VacMaster 10 and 20 ® Process 10 or 20 columns in parallel using...

Processing Options Automated Vacuum Processing Automated Positive Pressure Manifolds The Biotage Horizon 5000 is a three-position automated extraction system that conditions, loads the sample (20 mL to 2 L) and elutes the analytes all without user intervention. Biotage Extrahera workstations automate sample processing and do it with engineered precision. Positive pressure processing delivers reproducible, controlled flows in every position, even for viscous, slow flowing samples. Compatible with SPE and filtration disks and 6 mL column formats Clean, rugged system that won’t contaminate extracts...

Prior to analysis, an evaporation step is often needed for additional concentration or solvent exchange. TurboVap blowdown evaporators use a patented vortex evaporation system that increases the speed of evaporation by a factor of 10 compared with other techniques ® Faster evaporation and enhanced throughput Simple set up and method optimization Easy to use touch screen interface Match your extract volume and throughput needs Small footprint saves bench or hood space TurboVap LV » Evaporate up to 48 samples simultaneously » Flexible rack accommodates a wide selection of tubes ® TurboVap II »...

TurboVap -96 Dual » High throughput evaporation for 1 or 2 x 96-well plates » Independent settings for each position ® for maximum flexibility Adapts for 24 or 48 position evaporation TurboVap Systems to Streamline your Workflow In addition, Turbovap EH and P+ models are designed for streamlined workflow, allowing direct transfer of sample collection racks from processor to evaporator without the need to move tubes from one rack to another ® TurboVap EH accommodates 48 samples contained in tubes up to 18 x 75 mm (2 x 24 position racks). Transfer Biotage Extrahera collection racks direct to the...

Highlighted Application: Acrylamide AcrylamideHighlighted Application Acrylamide is formed when cooking carbohydrate rich food stuffs (eg breads and pastries, potato products and coffee) at high temperatures. Acrylamide Workflows from Biotage1. EN16618 Method This robust, widely used methodology uses a combination of two SPE columns to clean up and concentrate acrylamide in complex food samples: Application Notes Acrylamide in food (EN16618:2015 method) AN966 Method Scalability Although the published EN method uses relatively large column formats, the acrylamide extraction and clean up method...