Sample preparation workflow solutions from Biotage

7 Analyte extraction and extract clean-up 9 Processing options 15 Highlighted application: SVOC in drinking water 16 Highlighted application: SVOC in wastewater and groundwater 17 Highlighted application: oil & grease 18 Highlighted application: per and polyfluoroakyl substances 19 Highlighted application: pesticides & PCBs 20 Highlighted application: polycyclic aromatic hydrocarbons 21 Highlighted application: EPH fractionation The nature of an environmental sample can vary widely and each matrix can have any number of unique properties that can either help or hinder the preparation process....

Environmental analysis - sample preparation workflow solutions Sample Biotage® Horizon 5000 Sample preparation A word on sample matrices The way a particular sample is treated often depends on its origin. While several matrices may share certain attributes (pH, percent solids, etc.), each can have it’s own unique challenges that will need to be taken into account during the preparation process. Drinking water Characterized frequently by high amounts of solids as well as various chemical additives from the treatment process, these samples are some of the most complex there are. Often thought of...

Pre-treatment Sample preparation Homogenization is used to ensure that the sample matrix being used is representative of the location that you wish to report on and that the extraction is as effective as possible. Some samples may require only a simple bland in a food processor, but others may need something more. Biotage* Lysera is a bead homogenizer capable of preparing representative homogenates of even tough samples contain plant material or soil. Part number Fish/insects Garnet Metal Carbide Environmental analysis - sample preparation workflow so

QuEChERS QuEChERS (an acronym for Quick, Easy, Cheap, Effective, Rugged, and Safe) is an extraction and clean-up technique originally developed for pesticides in fruit and vegetable samples. It is frequently applied to soils and other environmental biosolids for similar analytes. QuEChERS extraction followed by clean-up using GCB is used for extraction of PFAS from soils (EPA Method 1633). QuEChERS extraction steps: 1. Salt assisted extraction into acetonitrile 2. Clean-up using dispersive (dSPE) or cartridge (cSPE) format Transfer supernatant QuEChERS Salt assisted extraction Transfer supernatant...

In the clean-up step » PSA removes sugars, fatty acids, organic acids, some pigments » C18(EC) removes lipids and other non-polar interferences » GCB removes some pigments, sterols QuEChERS clean-up products are available in pre-filled tubes, a range of flow-through cartridge formats, and bulk material. Table 2. dSPE and cSPE products for QuEChERS Packed 3 mL cartridge cSPE format Media Ratio Pre-filled 15 mL tube dSPE format ISOLUTE" ACAC General 200 mg ISOLUTE" ACAC Pigment 150 mg ISCLUTE" ACAC Waxed 350 mg ISCLUTE" EN High Pigment 500 mg Table 3. Single media cSPE products Table 4. Bulk media...

Analyte extraction and clean-up Analyte extraction and clean-up Biotage® Horizon 5000 Sample preparation The purpose of the extraction process is to isolate specific chemical compounds while simultaneously reducing the amount of interferences in the final sample that we are going to analyze. Broadly speaking, environmental samples for semi-volatile analysis can be broken down into two main categories: aqueous and solid. The primary focus of Biotage is on using solid phase extraction (SPE) which targets the analytes that you want while avoiding the interferences in the sample matrix. Solid phase...

Analyte extraction and clean-up Polymeric, water wettable, and pH 1-14 stable, with a range of functionality providing different selectivity. Packed into a range of SPE cartridge sizes, EVOLUTE SPE products are best suited for clean water samples. ® EVOLUTE EXPRESS ABN – for simultaneous extraction of acidic, basic and neutral analytes. Useful for extraction of PPCPs and endocrine disrupters EVOLUTE EXPRESS CX – for extraction of basic analytes EVOLUTE EXPRESS AX – for extraction of acidic analytes EVOLUTE EXPRESS WCX – for extraction of strongly basic analytes EVOLUTE EXPRESS WAX – for extraction...

Processing options Processing options Processing your samples is easy no matter if you want to use a cartridge or a disk. Biotage offers an array of options from simple manifolds to fully automated systems that are designed to meet your laboratory’s needs. Manual vacuum processing for cartridges 10 or 20 position Biotage VacMaster manifolds with flexible rack options to accommodate a variety of collection vessels ® Robust, inert construction materials Accessories for easier sample loading and cartridge drying Compatible with all cartridge sizes Manual vacuum processing for disks Biotage VacMaster...

Processing options Automated processing for general semi-volatile organics using disks Biotage Horizon 5000 are programmable, multipurpose SPE systems capable of processing aqueous samples directly from their original containers. The fast parallel processing of up to three large volume samples at one time maximizes the productivity of your laboratory. ® Designed to run both clean and dirty samples so labs can extract the whole sample Use 47 mm and 90 mm disks in either reusable or disposable disk holders or run 6 mL cartridges Accommodates 40-2000 mL sample volumes Small footprint to save lab...

Processing options Automated positive pressure systems Biotage PrepXpert-8 ® Automated 8-channel system for aqueous sample preparation Biotage PrepXpert-8 is a fully automated 8-channel sample preparation system designed to simplify aqueous sample workflows in environmental and PFAS analysis. Built for moderate to high-volume processing, Biotage PrepXpert-8 combines automation, traceability, and ease of use in a compact benchtop format. It supports regulated methods such as EPA 533, DIN EN 17892-09, and ES04506.1, making it ideal for routine testing and method development. ® PFAS-optimized flow...