Biotage PRESSURE+ 48 and PRESSURE+ 96 Positive Pressure Manifolds | Page 1 ® Biotage PRESSURE+ 48 and PRESSURE+ 96 ® Positive Pressure Manifolds Uniform Flow Unlike vacuum manifolds, in which faster flowing wells and columns dry before the processing run is completed, the independent ports of the PRESSURE+ manifolds deliver consistent output. This allows the same motive force to be applied to every cartridge and well, regardless of the contents. Ideal for Viscous Samples Biotage PRESSURE+ Positive Pressure manifolds allow for gas pressures of up to 100 psi, enabling greater pressure and force to be used with viscous samples. Smooth and immediate flow responses are achieved by adjusting gas pressure up or down through two regulators and a rotometer. ® PRESSURE+ 96 Accommodates all 96-Well Plate Formats Introduction Biotage PRESSURE+ manifolds deliver positive pressure, parallel processing for 96- and 48- well plates, 1 mL, 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency. ® Features and Advantages » Highly uniform flow from sample to sample for SPE, The self-adjusting upper manifold of the PRESSURE+96 manifold is compatible with all 96- and 48- well plate formats without the need to purchase supplementary gaskets. 1 mL tabless columns are also supported when processed in combination with the appropriate column holder (PPM-A96-CH). Biotage collection plates are recommended for the most consistent and reliable results. PRESSURE+ 48 for Cartridges The same self-adjusting technology utilized in the PRESSURE+ 96 manifold allows the PRESSURE+ 48 to utilize all columns up to 6 mL without the need to purchase supplementary gaskets. In addition, the unique design allows for between 1 and 48 columns to be processed without empty ports affecting flow rates. Tabless or flangeless columns should be used for full population and optimum sealing. The modular rack system accommodates most popular collection vessels to be used with each cartridge size. Modular Rack System By using stackable collection racks and extraction racks on the PRESSURE+ 48 manifold, many combinations are possible to accommodate varying methodologies. Racks only fit the system in one direction, ensuring consistent processing throughout. Protein Precipitation and Supported Liquid Extraction Improved reproducibility, accuracy and extraction efficiency, even with viscous samples The intuitive Biotage PRESSURE+ is easily incorporated into laboratory work flow. Simply stack 96-well plates and collection plates (for PRESSURE+ 96) or column rack and collection racks (for PRESSURE+ 48) on the platform tray using the positioning markers and slide the tray under the gasket until it hits the stop. Rocker switches pressed simultaneously on the side/front of the unit lowers the gasket and compress it onto the plates/ columns. This simultaneous depression of the rocker switches is a safety feature that ensures hands are kept clear of moving parts during operation. Parallel processing for increased sample throughput Easy to set-up and use with just a gas source Universal designs accommodate all 96- and 48-well plates, or 1 mL, 3 mL and 6 mL SPE columns Built-in safety features ensure ease of use and safe operation

Biotage PRESSURE+ 48 and PRESSURE+ 96 Title Positive Truncated Pressure if Neccessary... Manifolds | |Page Page10 2 ® Properties ofInformation RENSA RP Ordering ™ Average Particle size Part Number Description Average pore diameter Biotage PRESSURE+ Average pore volume 96 Part Number Average surface pore area 400 m /g PPM-96 Biotage PRESSURE+ 96 Positive Type of interaction Styrenic Pressure Manifold 96 Well. Includes Cross-linking degree ~ 50 % 1 x 1 mL 96 well collection plate, 1 x 2 mL 96 well collection plate, Working pH range 1–14 1 x 10 mL 24 well collection plate Average swelling 60% (toluene)...