NightShade LB 985
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NIGHTSHADE LB 985 IN VIVO PLANT IMAGING SYSTEM Visualize what plant biology has been hiding

THE NIGHTSHADE LB 985 IN VIVO PLANT IMAGING SYSTEM Better Imaging. Better Understanding The NightShade LB 985 In Vivo Plant Imaging System is a modular, easy to use optical imaging system dedicated to in vivo analysis of plants. Equipped with an absolutely lighttight cabinet and a deeply cooled CCD camera it enables sensitive luminescence and fluorescence monitoring in tissues, seedlings and whole plants. The camera can be attached either to the top or the side of the darkroom – the sample chamber – to enable imaging from above and from the side. The side position of the camera enables...

Smart imaging chamber - full compatibility for diverse applications The Nightshade provides a light-tight imaging chamber that you can set up according to your experimental needs ■ multi-position camera - the camera can be mounted either on top of the instrument to take images from above or laterally for side view images, enabling you to maintain seedlings upright in their natural vertical orientation. ■ easy handling of samples - drawer-like base plate for easy exchange of samples and accessories (e.g. turntables). ■ direct sample chamber access - light-tight ports for the introduction of...

BERTHOLD TECHNOLOGI “The controlled environment and exquisite sensitivity provided by the Nightshade In Vivo Plant Imaging System allows you to perform the experiments that were difficult in the past.” Dr, Anselm Berthold, Berthold Technologies

APPLICATIONS & SOFTWARE When cell biology meets physiology In the plant research field, there´s an increasing need for in vivo imaging that enables researchers to visualize multiple events simultaneously and to detect and localize them in real-time. Plant researchers can utilize both, bioluminescent and fluorescent agents to collect a wealth of information out of their samples like concentration, protein-protein interactions and metabolic activity. However, it is the combination of these sensitive and affordable detection chemistries with advanced macroscopic visualization methods that...

Better understanding of circadian rhythms Endogenous biological clocks drive daily rhythms enabling plants to anticipate environmental changes as well as to coordinate and adapt their physiology in a synchronized manner. The NightShade provides the controlled experimental setup required to monitor temporally regulated events automated and in real-time. With NightShade In vivo imaging, you benefit from n real-time monitoring: the NightShade provides easy setup of time-course studies according to your research needs. n better data: the controlled experimental setup of the system enables you...

Better monitoring of stress factors Delayed fluorescence (or afterglow) acts as an indicator not only for chlorophyll content, but also for the physiological state of the plant. The NightShade simplifies analysing the impact of herbicides, pathogens, drought and other stress factors that can act on the chloroplasts and thereby alter the delayed fluorescence reaction. With NightShade In vivo imaging, you benefit from n igh sensitivity: the sensitive deeply-cooled CCD h camera of the NightShade enables the detection of low light intensities. n ser friendly software: simple data acquisition u...

BERTHOLD TECHNOLOGI The user-friendly, IndiGO™ software controls the instrument and makes image processing easy. It provides the following innovative features: ■ Easy filter management The filter management system of IndiGO™ makes it simple to use different dyes in your experimental setup. Dedicated filters for both, excitation and emission are selected via a filter inventory that provides you with information about the filter properties. ■ Daylight simulation A set of 2 LED panels, 4 colours each can be mounted at different heights inside the imaging chamber for daylight simulation. With...

THE NIGHTSHADE LB 985 IN VIVO SYSTEM AT A GLANCE Outside view Top view camera position Light-source with excitation filter slider Emission filter wheel Light-tight imaging chamber Lockable door Side view camera position Inside view LED panels Water distribution manifold Excitation source Flange with light-tight ports Optional turntable Base plate drawer

BERTHOLD TECHNOLOGI With the Nightshade in Vivo imaging System your lab can be ready for many types of experiments LED Panel A LED Panel B ■ LED panels for daylight simulation Two different LED panels can be mounted inside the imaging chamber to simulate daylight with both, spectral and intensity gradients. Each panel is equipped with 4 different LED colours. ■ anti-condensation table The Anti-Condensation Table for up to 9 Petri dishes utilizes a water heating for temperature control. An integrated fan system creates a constant, circular air flow above the Petri dishes, preventing...

Options Accessories Sideview Kit 60798 LED plant growth illumination panel A: blue/green/red/far-red 56589-01 LED plant growth illumination panel B: blue/white/red/far-red 56589-10 Cooling/heating unit for LED plant growth illumination, 230 V 56695 Cooling/heating unit for LED plant growth illumination, 110 V 56706 Turntable for square Petri dishes 100 x 100 mm 56625-01 Turntable for square Petri dishes 130 x 130 mm 56625-02 Turntable for DeWit tubes 56625-03 Turntable Base Unit 56625-04 Turntable attachement for square Petri dishes 100 x 100 mm 56599-01...

BERTHOLD TECHNOLOGI IK CCD Camera 1024 x 1024 pixels slow scan mode pixel size: 13 x 13 pm2 Thermoelectrical air cooling down to -70oC Back-lit midband-coated full frame chip Spectral range: 350 - 1050 nm Quantum efficiency: 90 % at 620 nm Pixel binning: variable, up to 16 x 16 Exposure times: from ms to hours Lens 25 mm, f 0.95, C-mount for most efficient light collection Field view: Standard lens: 270 x 270 mm2 (XT version: 320 x 320 mm2) Macro lens: 130 x 130 mm2 Light Source Halogen lamp, 75 W, 340 - 750 nm Software controlled lamp stabilisation Imaging Chamber Dimensions:...