SAPPHIRE™ FL BIOMOLECULAR IMAGER
12Pages

SAPPHIRE™ FL BIOMOLECULAR IMAGER UNLIMITED POSSIBILITIES, UNCOMPROMISING PERFORMANCE

SAPPHIRE FL BIOMOLECULAR IMAGER Flexibility with uncompromising performance – From in vitro molecular assays to in vivo imaging The Sapphire FL is the ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, the Sapphire FL easily adapts to a lab’s changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5–1000 µm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia ports for imaging living animals, chemiluminescence detection through the Chemiluminescence Module and...

APPLICATION FLEXIBILITY

IMAGE ALL THE SAMPLES YOU CAN IMAGINE Innovation-driving performance – High resolution imaging and wide depth of field enable imaging of many sample types 4 CM CLEARANCE Enables imaging of samples with depth 5 ANESTHESIA PORTS Compatible with standard anesthesia kits LARGE 25 cm X 25 cm FIELD OF VIEW Get More Done – Scan as many as 6 membranes (9 x 7 cm) or 6 96-well plates at a time. 4 cm of clearance above the glass allows for small animal imaging and tissue imaging. SAMPLE TYPES PVDF Membranes | Nitrocellulose membranes | 384-well plates 96-well plates | 12-well plates | 6-well plates |...

SAMPLE FLEXIBILITY HIGH RESOLUTION IMAGING – UP TO 5 MICRON RESOLUTION Z PLANE ADJUSTMENT FOR SAMPLES WITH MULTIPLE FOCAL PLANES Whole Slide Imaging – Screen slides before microscopic analysis by imaging multiple slides at a resolution of 5 microns. The adjustable focal plane enables scanning of thick samples. Find the Best Data- Adjustable laser focus from -1 mm below to +6 mm above the glass surface. The adjustable focal plane allows for optimal imaging of your sample, even when offset from the glass. Gel imaging 96-well plate imaging Western blotting applications A. Mouse lung tissue...

WHERE WILL YOUR RESEARCH TAKE YOU TODAY? Customization and upgradability – Pick the modules that support your research NO LIMITS – EASILY SWAP LASERS AND FILTERS FOR EXPANDED DYE FLEXIBILITY A A. Remove optical module from system. B. Remove and replace filter. C. Load new laser and filter combination into the system. Optical Modules Optical modules 488 Standard Optical Module 375 Custom Optical Module 532 Standard Optical Module 450 Custom Optical Module 638 Standard Optical Module 488 (YFP) Custom Optical Module 685 Standard Optical Module 784 Standard Optical Module 532 (Propidium Iodide)...

NIR FLUORESCENCE AzureSpectra™ 700 | AzureSpectra 800 | Cy® 5.5 | Cy7 | Alexa Fluor® 680 Deep Purple™ | DyLight™ 650 | DyLight 680 | DyLight 755 | DyLight 800 CHEMILUMINESCENCE WHEN YOU NEED IT Upgrade to the Sapphire FL Chemiluminescence Module. A ECL Plex™ | Ethidium Bromide | GelStar® | IRDye® 650 | IRDye 680LT IRDye 680RD | IRDye 700DX | IRDye 750 | IRDye 800CW | IRDye 800RS Ponceau | Qdot® 525 | Qdot 565 | Qdot 585 | Qdot 605 | Qdot 705 | Qdot 755 VISIBLE FLUORESCENCE AzureRed™ | AzureSpectra 488 | AzureSpectra 550 | AzureSpectra 650 Alexa Fluor 488 | Alexa Fluor 546 | Alexa Fluor 555...

Broad dynamic range and exceptional sensitivity enable enhanced quantitative data generation DISTINGUISH SUBTLE DIFFERENCES IN EXPRESSION WITH EXTENDED DYNAMIC RANGE (EDR) Extended dynamic range, when selected, allows imaging of both bright and weak bands without experiencing saturation. This is ideal for samples that feature strong and weak expressing proteins. EDR extends dynamic range to 24 bits of data. 685 Standard Optical Module Log Integrated Intensity High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes. 204.8 102.4 532 Standard Optical Module...

PERFORMANCE WITHOUT COMPROMISE Be confident that every scan will be accurate and reproducible. Z 2-fold dilution dot blot scanned across nine separate regions of the imaging area, as shown. CV of individual dot intensities was less than 5% across the imaging area. 4-CHANNEL FLUORESCENT IMAGING – SEE MORE IN A SINGLE SAMPLE Quickly and easily swap optical modules to create 4-channel images. PHOSPHOR IMAGING – IMAGE STORAGE PHOSPHOR SCREENS WITH HIGH SENSITIVITY Storage phosphor screens are imaged, digitized and ready for quantitation. Log Integrated Intensity REPRODUCIBILITY AND UNIFORMITY –...

SPECIFICATIONS AND ORDERING INFORMATION Sapphire FL Biomolecular Imager Laser Based Scanning System Part number Standard Optical Modules Part number 488 Standard Optical Module Scanning area 532 Standard Optical Module Scanning modes Simultaneous, Sequential, Extended Dynamic Range (EDR) 638 Standard Optical Module 685 Standard Optical Module Image output 784 Standard Optical Module Phosphor Imaging Standard Optical Module Maximum scanning speed Animal imaging Compatible with commercially available anesthesia systems 375 Custom Optical Module 450 Custom Optical Module 488 (YFP) Custom...

Part number Standalone Laser Options (Does not include emission filter) Part number Sapphire Eraser Custom laser options available upon request Standalone Emission Filter Options (Does not include laser module) IS4008 Custom emission filter options available upon request Designed to erase signal from phosphor imaging screens Sapphire FL Chemiluminescence Module Add Chemiluminescence Module to Sapphire FL

6747 Sierra Court, Suites A-B • Dublin, CA 94568 USA Phone: 925.307.7127 • Fax: 925.905.1816 info@azurebiosystems.com • www.azurebiosystems.com Copyright © 2023 Azure Biosystems. All rights reserved. The Azure Biosystems logo, Azure Biosystems®, AzureSpectra™, Radiance®, Radiance Plus®, and Sapphire™ are trademarks of the Company. CellROX® is registered trademark to Life Technologies, Inc. CellTracker™ is trademark of Invitrogen. Flamingo™ is trademark of Bio-Rad. Alexa Fluor®, DyLight™, Qdot®, SYBR®, and SYPRO® are trademarks of Thermo Fisher Scientific. Cy2®, Cy3, Cy5, Cy5.5, and Cy7 are...