Introduction The Argo-POWERHM is specifically designed to assess and follow the performances of fluorescence-based imaging systems, such as wide-field, confocal, spinning disk and other types of microscopes, with high magnification (HM) objectives. This product is composed of a calibrated optical power meter, an Argo-HM patterned glass and Daybook, their companion image analysis software. The calibrated optical power meter is designed to measure the optical power of light sources at the sample plane in microscopy setups. The Argo-HM glass is a special glass substrate with different fluorescent patterns embedded inside. Daybook software allows to analyze and extract data (maps, graphs and metrics) from images of the patterns, in order to control significant parameters of your fluorescence imaging system. Objective compatibility The glass part is compatible with dry and oil immersion objectives. Water immersion is possible, but continuous exposure longer than five minutes should be avoided. The optical power meter is only compatible with dry objectives. Argolight - 2019 - [email protected] - www.argolight.com

Spec sheet ARGOLIGHT ARGO-POWERHM device for fluorescence imaging systems 03/2019 Patterns position inside the glass Patterns are positioned (170 ± 5) μm below the top glass surface, on a horizontal plane which flatness is within ± 5 mrad. This emulates the presence of a microscope coverglass, having a thickness of (170 ± 5) μm and a refractive index of (1.5255 ± 0.0015) at 546.1 nm. The maximum relative positioning error is ± 110 nm in XY and ± 110 nm in Z within each individual pattern. The thickness (in the Z direction) of these patterns is about (600 ± 200) nm FWHM (Full Width at Half Maximum)....