Calibration Light Source for Cytometry quantiFlash® Key Facts Cytometer calibration with light pulses Routine detector / PMT performance test Distinguish dim populations from noise Calibrate intensity scales to absolute units Ideally suited for quality control, service, and development

Calibration Light Source for Cytometry quantiFlash - a calibration light source - provides consistent and uniform light pulses that are ideally suited for cytometer calibration. The intensity and duration of the pulses is freely adjustable. In contrast to beads, light pulses have a very small intrinsic coefficient of variation, are stable for any length of time and their quality does not depend on storage conditions. For cytometers the fluorescence beads are primarily a source of light. Light is emitted from a bead as it passes through the laser beam in the focus of the flow cell of the cytometer....

Typical Applications Comparison and Alignment of Flow Cytometer Results 10 quantiFlash makes it possible to calibrate the intensity scale of each channel in a flow cytometer to a meaningful absolute unit, e.g. a standardized photonic scale such as Spe (number of detected statistical photoelectrons). This allows direct quantitative comparison between different devices. A calibrated intensity scale makes it possible to transfer measurement results between flow cytometers and therefor improves the comparability of results obtained from different flow cytometers. before normalization after normalization...

Standard (bead) calibration protocols as well as specific quantiFlash protocols, e.g. from NIST, can be used with quantiFlash. A list of common protocols can be found on our website. Light pulses with almost no statistical variations allow for more accurate measurements and calculations of important cytometer parameters, such as Detection Efficiency (Q), Background (B), Statistical PhotoElectrons (Spe), Fluorescence Intensity, Mean Equivalent Soluble Fluorescence (MESF), or Equivalent number of Reference Fluorophores (ERF). Performance Test of PMTs / Detectors 10 The perfect linearity of quantiFlash...

Calibration Light Source for Cytometry quantiFlash Mounting Kit for BD Available 10 quantiFlash comes with 2 fibers. The fiber ends have to be interfaced with the flow cytometer. A dedicated apdapter for BD instruments is available from APE, including: LSR II FACSAria FACSCanto FACSCalibur LSRFortessa An easy to use software interface is part of every quantiFlash. Own automated measurement routines and pulse shapes can be programmed using a Pro software version. TCP/IP Programming Interface 10 A TCP/IP-based standard software interface by APE makes it straight forward to set up remote control...

quantiFlash Specifications quantiFlash Pulse Duration Events (Repetition Rate) Repetition Rate Precision Pulse Shape Variable (Gaussian as default) Pulse Amplitude Amplitude Precision Fiber Coupling 2 x fibers with f-SMA connector Options Advanced PC software Optical attenuators Customized fibers and connectors Mounting Kit for BD Instruments Dimensions and Power Dimensions Power Li-ion battery included, rechargeable USB powered Fiber Output BD Flow Cell Adapter

Your Partner in Ultrafast, Light, Measurement 10 Located in Berlin / Germany, APE is a worldwide leading supplier in the field of laser pulse diagnostics and wavelength conversion. Our product portfolio includes measurement technology for pulsed light and lasers, optical parametric oscillators (OPOs), optical parametric amplifiers (OPAs), and systems for harmonic generation. Our ultrashort pulse diagnostics line covers autocorrelators for pulse width measurements, spectrometers, and other equipment for measuring and characterizing femtosecond and picosecond laser pulses. Light-pulse Calibration...