picoEmerald

Product catalog summary

Introduction
APE's picoEmerald is a fully automated two-color laser source designed for advanced imaging and spectroscopy techniques such as Coherent anti-Stokes Raman Spectroscopy (CARS), Stimulated Raman Spectroscopy (SRS), Second Harmonics Imaging (SHG), and Surface Enhanced Hyper Raman Spectroscopy (SEHRS). It is particularly suited for Raman-based microscopy and spectroscopy, offering high spectral resolution and low noise signals. The system is a turn-key device with integrated components like a spectrometer and electric optical modulator, making it valuable for both Raman applications and quantum photonics research.

Specifications and Features
The picoEmerald emits narrow bandwidth picosecond pulses, providing an optimal balance between spectral resolution and signal intensity. It is equipped with a spectral slicing unit (pulseSlicer) for precise excitation in quantum dot research, enhancing its utility in quantum photonics.

Applications
The picoEmerald is used in various scientific fields, including the study of lipid droplets in mammalian eggs, emulsions replicating human breast milk, and label-free characterization of amyloid plaques in Alzheimer's disease models. It is also applied in the analysis of protein interactions, microstructure of food products, and the study of rare neurological diseases.

Scientific References
The document lists numerous scientific studies from 2019 to 2021 that utilize the picoEmerald for applications in CARS, SRS, and fluorescence imaging. These studies cover a wide range of topics, from cancer diagnosis and plant cell wall imaging to quantum dot research and metabolic imaging.

Conclusion
The picoEmerald is a versatile tool for researchers in fields requiring high-resolution, label-free imaging and spectroscopy. Its integration into various scientific studies highlights its effectiveness and adaptability in cutting-edge research.

Overview: This document is a compilation of research articles and book chapters focusing on advanced microscopy techniques such as SHG-microscopy, SRS, and CARS. These techniques are applied to study biological tissues and cellular processes.

1. SHG-Microscopy:
- Automated Quantification of Fibrillar Structures: Discusses a method for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues using SHG-microscopy.
- Vibrational Imaging of Proteins: Explores the use of stimulated Raman scattering microscopy for imaging newly synthesized proteins in live cells.

2. SRS (Stimulated Raman Scattering):
- Epi-Detected SRS Microscopy: Describes the use of long-wavelength excitation in SRS microscopy for biomedical optics and 3-D imaging.

3. CARS (Coherent Anti-Stokes Raman Scattering):
- Lipid Droplet Remodeling: Examines the remodeling of lipid droplets during lipolysis and growth in adipocytes.
- Collagen Fiber Orientation: Provides a quantitative assessment of collagen fiber orientations in soft biological tissues.
- Single-Pass Picosecond Supercontinuum: Details the use of a single-pass picosecond supercontinuum-seeded optical parametric amplifier in CARS microscopy.

4. Books and Chapters:
- Quantitative Imaging of Lipid Metabolism: Discusses methods for imaging lipid metabolism in yeast, including 4D analysis and high-content screens.
- Collagen Fiber Morphologies: Introduces novel methodologies for quantifying collagen fiber morphologies in human arterial walls.
- Label-Free Imaging of Adipogenesis: Covers the use of CARS microscopy for label-free imaging of adipogenesis.

Contact Information:
APE Angewandte Physik & Elektronik GmbH, Berlin, Germany. Contact details provided for further inquiries.

Note: APE GmbH follows a policy of continued product improvement, and specifications are subject to change without notice.

Catalog excerpts

picoEmerald - A Reference List Imaging and Spectroscopy with a Tunable Two-Color-Source Image Courtesy: Sang-Won Lee & Joo Hyun Park. Multimodal nonlinear microscopic images incl. TPEF, SHG, CARS and merged image.

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APE’s picoEmerald - A Reference List Introduction APE’s picoEmerald is an fully automated two-color laser source. It has been specifically designed to meet the requirements of CARS, SRS, multimodal imaging, SHG/multiphoton fluorescence, and similar methods. The platform is specially designed for Raman-based microscopy and spectroscopy techniques that allow samples to be investigated without labeling. These techniques are based on the intrinsic vibrational contrast between various molecules and their differentiating atom bounds. The narrow bandwidth of picosecond pulses emitted by picoEmerald,...

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Scientific References (Selection) (Year) Title, Journal, DOI Author Application (2021). Lipid droplets in mammalian eggs are utilized during embryonic diapause Proceedings of the National Academy of Sciences DOI: https://doi.org/10.1073/pnas.2018362118 (2021). Emulsions containing optimum cow milk fat and canola oil mixtures replicate the lipid self-assembly of human breast milk during digestion Arena, Roberta; Bisogno, Simona; Gasior, Lukasz; Rudnicka, Joanna; Bernhardt, Laura; Haaf, Thomas; Zacchini, Federica; Bochenek, Michal; Fic, Kinga; Bik, Ewelina; Baranska, Malgorzata; BodzonKulakowska,...

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(Year) Title, Journal, DOI Author Application (2020). Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells Du, Jiajun; Su, Yapeng; Qian, Chenxi; Yuan, Dan; Miao, Kun; Lee, Dongkwan; Ng, Alphonsus H. C.; Wijker, Reto S.; Ribas, Antoni; Levine, Raphael D.; Heath, James R. & Wei, Lu Hansen, Daniel; Brewer, Jonathan R.; Eiler, Johannes; Komjani, Niloufarsadat Mirmahdi; Hansen, Kristoffer & Thormann, Esben Huo, Zepeng; Chen, Gang; Geng, Yijia; Cong, Lili; Pan, Lingyun; Xu, Weiqing & Xu, Shuping Kim, Jin Young; Liu, Zezhou; Weon, Byung Mook; Cohen, Tal; Hui, Chung-Yuen; Dufresne,...

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(Year) Title, Journal, DOI Author Application (2020). Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits Suhar, Riley A.; Marquardt, Laura M.; Song, Shang; Buabbas, Hana; Doulames, Vanessa M.; Johansson, Patrik K.; Klett, Katarina C.; Dewi, Ruby E.; Enejder, Annika M. K.; Plant, Giles W.; George, Paul M. & Heilshorn, Sarah C. Wagner, Carina; Hois, Victoria; Pajed, Laura; Pusch, Lisa-Maria; Wolinski, Heimo; Trauner, Michael; Zimmermann, Robert; Taschler, Ulrike & Lass, Achim Wilson, Liam T.; Tipping, William J.; Jamieson, Lauren E.; Wetherill, Corinna; Henley,...

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(Year) Title, Journal, DOI Author Application (2019). The role of tissue remodeling in mechanics and pathogenesis of abdominal aortic aneurysms Niestrawska, Justyna A.; Regitnig, Peter; Viertler, Christian; Cohnert, Tina U.; Babu, Anju R. & Holzapfel, Gerhard A. Ramachandran, Prasanna V.; Savini, Marzia; Folick, Andrew K.; Hu, Kuang; Masand, Ruchi; Graham, Brett H. & Wang, Meng C. Schiessl, Konstanze T.; Hu, Fanghao; Jo, Jeanyoung; Nazia, Sakila Z.; Wang, Bryan; Price-Whelan, Alexa; Min, Wei & Dietrich, Lars E. P. Shahmoradian, Sarah H.; Lewis, Amanda J.; Genoud, Christel; Hench, Jurgen; Moors,...

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(Year) Title, Journal, DOI Author Application (2018). Variability in responses observed in human white adipose tissue models Journal of Tissue Engineering and Regenerative Medicine DOI: https://doi.org/10.1002/term.2572 (2018). Invited Article: Comparison of hyperspectral coherent Raman scattering microscopies for biomedical applications Abbott, Rosalyn D.; Borowsky, Francis E.; Alonzo, Carlo A.; Zieba, Adam; Georgakoudi, Irene & Kaplan, David L. Bocklitz, T.; Meyer, T.; Schmitt, M.; Rimke, I.; Hoffmann, F.; von Eggeling, F.; Ernst, G.; Guntinas-Lichius, O. & Popp, J. Cheng, Qian; Wei, Lu; Liu,...

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(Year) Title, Journal, DOI Author Application (2018). Integrated mapping of pharmacokinetics and pharmacodynamics in a patientderived xenograft model of glioblastoma Nature Communications DOI: https://doi.org/10.1038/s41467-018-07334-3 (2018). On-demand generation of background-free single photons from a solid-state source Applied Physics Letters DOI: https://doi.org/10.1063/1.5020038 (2018). Invited Article: Visualizing protein synthesis in mice with in vivo labeling of deuterated amino acids using vibrational imaging Randall, Elizabeth C.; Emdal, Kristina B.; Laramy, Janice K.; Kim, Minjee;...

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(Year) Title, Journal, DOI Author Application (2017). Studies for improved understanding of lipid distributions in human skin by combining stimulated and spontaneous Raman microscopy Klossek, A.; Thierbach, S.; Rancan, F.; Vogt, A.; Blume-Peytavi, U. & Ruhl, E. Lafeta¡, Lucas; Cadore, Alisson R.; Mendes-de-Sa, Thiago G.; Watanabe, Kenji; Taniguchi, Takashi; Campos, Leonardo C.; Jorio, Ado & Malard, Leandro M. Li, Xuesong; Jiang, Meijuan; Lam, Jacky W. Y.; Tang, Ben Zhong & Qu, Jianan Y. Lombardini, Alberto; Andresen, Esben Ravn; Kudlinski, Alexandre; Rimke, Ingo & Rigneault, Hervè Mah, Pei; Novakovic,...

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(Year) Title, Journal, DOI Author Application (2016). Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue Nature Communications DOI: https://doi.org/10.1038/ncomms10940 (2016). Continuous Grading of Early Fibrosis in NAFLD Using Label-Free Imaging: A Proofof-Concept Study PLOS ONE DOI: https://doi.org/10.1371/journal.pone.0147804 (2016). Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging Nature Communications DOI: https://doi.org/10.1038/ncomms13283 (2016). Probing Two-Photon Molecular Properties...

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(Year) Title, Journal, DOI Author Application (2014). Dopamine Signaling Regulates Fat Content through B-Oxidation in Caenorhabditis elegans PLOS ONE DOI: https://doi.org/10.1371/journal.pone.0085874 (2014). Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source. {Nature Cell Biology} DOI: https://doi.org/10.1038/ncb2922 (2014). Transient absorption microscopy of gold nanorods as spectrally orthogonal labels in live cells Barros, Alexandre Guimaraes de Almeida; Bridi, Jessika Cristina; de Souza Bruno Rezende; de Castro Junior Celio; de Lima Torres Karen...

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picoEmerald

picoEmerald

Catalog excerpts

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