deltaEmerald Dual Color SRS Imaging Dual Color SRS Imaging The new deltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual color SRS (DC-SRS) scheme. It is aimed for users at the forefront of SRS microscopy, given the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands. Two Stokes pulses, separated by 85 cm-1 and modulated at different frequencies are overlapped with the tunable Pump pulse. The pulse length of about 1 ps, 10 cm-1 ... 15 cm-1 bandwidth and several 100 mW output power in each beam are ideal parameters for Coherent Raman imaging. The pulses of all three beams are shot noise limited due to solid state laser design and proven OPO technology. The shot noise limited -162 dBc/Hz above 1 MHz (limited by the noise measurement setup with 4.8 mA) allows for fast image acquisition and superior signal-to-noise ratio. Fully automated tuning, power control and temporal overlap of all three beams are given. Additional a ~100 fs-output at 1030 nm is provided for efficient SHG and two-photon imaging. Innovative SRS scheme for simultaneous excitation of two vibrational band Tunable Pump and both Stokes pulses are spatially and temporally overlapped Spectral distance of Stokes 85 cm-1 Proven OPO technology with shot noise limited performance with -162 dBc/Hz above 1 MHz Additional femtosecond output at 1030 nm for optimum SHG and TPF excita

deltaEmerald Dual Color SRS Imaging 1. Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology. The 85 cm-1 difference is chosen to address CH2 & CH3 with 2845 cm-1 and 2930 cm-1 at their peak position. 2. Background removal of non-resonant artefact's, especially important for weak signals in the fingerprint region. 3. Cosmetics / pharmaceutical imaging and further applications. Colon, courtesy of LightCore Technology Biospie, courtesy of LightCore Technology Specifications Wavelength Stokes 1 W avelength Pump (OPO Signal) Specifications P ower Stokes 1 P ower Pump...

deltaEmerald Specifications S pectral bandwidth Pump and Stokes beam s S hot noise limited (-162 dBc/Hz) > 1 MHz (Pump and Stokes ) Beam Parameters Pointing stability Additional Femtosecond Output Wavelength

Features Integrated modulators for Stokes beams Built-in EOM with resonant fixed frequency of 20 MHz and 13 MHz Integrated for Pump and Stokes, independent for each beam A utomated compensation for microscope dispersion Remote control Mechanical dimensions (L x W x H) / weight: deltaEmerald L aser control unit Further requirements: Power consumption Operating temperature 2 0°C … 25°C, air conditioning (stability ± 1°C) recommended Contact APE Angewandte Physik & Elektronik GmbH Plauener Str. 163-165|Haus N|13053 Berlin|Germany T: +49 30 986 011-30 F: +49 30 986 011-333 E: [email protected]...