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Innovative & Flexible Laser Microscopy Systems

Innovative & Flexible Laser Microscopy Systems
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Innovative & Flexible Laser Microscopy Systems - 1

www.andor.com Innovative & Flexible Laser Microscopy Systems Microscopy Systems Discover New Ways of Seeing™

Innovative & Flexible Laser Microscopy Systems - 2

Microscopy Techniques Microscopy Applications Revolution components and systems benefit from common features across applications. Whether in confocal, photo-bleach/activation or TIRFM imaging this means state of the art performance, flexible component-oriented supply and an open-minded approach to systems and technically demanding solutions. As we continue to provide wide-ranging support for key hardware components, we also try to increase your choice through expanding our software compatibility. We are actively involved in making the bridge from iQ to MatLab, Imaris or Image-J and...

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Innovative & Flexible Laser Microscopy Systems - 3

Laser Microscopy Andor Revolution Andor Revolution provides a framework for our range of laser-illuminated microscopy solutions, including spinning disk confocal, TIRFM and photobleach and activation modalities. Our spinning disk, live cell confocal instrument, illustrated below, combines Andor’s award winning iXonEM+ EMCCD cameras, solid-state laser combiner and the renowned Yokogawa CSU-X1 confocal scanner “inside”. Andor has established a global distribution agreement with Yokogawa Electric Corporation and harnessed our optoelectronics engineering and manufacturing knowledge to create...

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Innovative & Flexible Laser Microscopy Systems - 4

www.andor.com Microscopy Systems Products iXonEM+ Providing Single Photon Sensitivity • DU-897 • DU-888 • DU-885 • DU-860 512 x 512 pixels; 16 x 16ìm; QE >90%, the ultimate combination of sensitivity and speed 1024 x 1024 pixels; 13 x 13ìm; QE >90%, resolution and exceptional sensitivity combined 1004 x 1002 pixels; 8 x 8ìm; QE >60%, 35 MHz readout, high resolution intra-cellular imaging 128 x 128 pixels; 24 x 24ìm; QE >90% for high speed applications e.g Ca2+ flux All iXon detectors benefit from: a) minimized dark current from unparalleled –90°C TE cooling – especially critical for...

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Innovative & Flexible Laser Microscopy Systems - 5

Laser Combiner & Multi-Port Switch Uniquely Flexible • Integral AOTF for laser selection and modulation (cross talk <80dB) • Unique Active blanking, synchronized to camera-minimizes specimen exposure • Compact 4U 19” rack mount enclosure • Quiet, cool, low power consumption • Long life solid state lasers with excellent stability (typical +/- 0.4% pk to pk) • ALC allows Gas laser integration e.g. Ar ion or mixed gas • Up to 5 solid state lasers • Wavelengths covering the visible spectrum •Multiple ports for all laser lines - CSU, FRAPPA and TIRF • Delivers 100% of laser power to one of three...

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Innovative & Flexible Laser Microscopy Systems - 6

www.andor.com FRAPPA Photo-Bleaching & Activation Features FRAPPA - Our latest innovation in laser microscopy is named after an acronym of the techniques which it supports. Fluorescence Recovery After Photo bleach (FRAP) and Photo Activation (PA) are established imaging protocols in which a computer-steered laser beam is used to photo-bleach or photo-active a user-defined region in the specimen. Andor’s FRAPPA uses a dual galvanometer scan head to provide a computer-steered laser beam delivery system. It can be configured in line with a CSU and/or camera and operates in two modes. •...

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Innovative & Flexible Laser Microscopy Systems - 7

Camera Port Adapters Single & Dual Port • Magnification X = 1.0, 1.2, 1.5, 2.0 - other by request • Compatible with filter wheel or CSU+filter wheel • Enables simultaneous two-camera exposure with discrimination by wavelength or polarization. A 100% mirror can be included for easy camera switching. • Magnification X = 1.0, 1.2, 1.5, 2.0 - other by request • Compatible with ‘C’/’CS’ lenses, C-mount, filter wheel or CSU+filter wheel • Magnification select in each arm • Mounting cassettes ‘store adjustment’ simplifying exchange and minimizing readjustment Single Port Coupler Dual Port Coupler...

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Innovative & Flexible Laser Microscopy Systems - 8

www.andor.com www.andor.com Discover New Ways of Seeing™ MB-07/09 Microscopy Techniques Fluorescence Recovery After Photo bleach (FRAP) and Photo Activation (PA) provides a tool for precisely controlled and targeted application of the selected laser beam to initiate photo-effects, which include (FRAP) fluorescence recovery, (FLIP) rate of loss with repeated bleaching, (PA) activation and even ablation (with Q-switched UV laser). Imaging the effects reveals qualitative and quantitative information about the cellular structures of interest and their environment. For example, photo-activated...

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