chromatography and spectroscopy
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chromatography and spectroscopy - 1

chromatography and spectroscopy consumables and spare parts

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chromatography and spectroscopy - 2

table of contents – chromatography Table of contents 1. Syringe filter units 2. Titan syringe filter 3. Target syringe filter 4. Solid phase extraction (SPE) by Macherey-Nagel 5. Thin layer chromatography (TLC) 6. Vials, micro inserts and closures for HPLC 7. Vials and closures for GC 8. Crimping tools 9. Storage system for HPLC and GC columns 10. Guard Columns by Altmann Analytik 11. HPLC Columns by Altmann Analytik 12. HPLC Columns by Agilent 13. Kromasil HPLC Columns 14. Chiral Columns by Daicel 15. HPLC Columns by Grace 16. HPLC Columns by Macherey-Nagel 17. HPLC Columns by Merck 18....

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chromatography and spectroscopy - 3

syringe filter units 1. Syringe filter units Syringe filters are used for filtration of suspended matter from liquid sample or gases. Rapid purification and removal of particles is very simple: just place the filter on a syringe, and you are ready for filtration. Contamination of sensitive instrumentation by solid impurities can be avoided. Also the lifetime of chromatographic columns and equipmend can be increased. pore size

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chromatography and spectroscopy - 4

syringe filter units Selection of the appropriate filter FILTER SIZE: sample volume Filter size PORE SIZE: general applications, hplc columns with >3 µm particles uhplc, core-shell and hplc columns with <3 µm particles TYPE OF MEMBRANE: properties of the samples Aqueous, polar, hydrophilic with low particle load Aqueous, polar, hydrophilic with high particle load Semi-polar, e.g. HPLC eluents Sample contains proteins (low bonding capacity of the proteins) Sample contains proteins (high bonding capacity of the proteins) Strongly acidic or alkaline Organic, non-polar, hydrophobic with low...

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chromatography and spectroscopy - 5

titan syringe filter 2. Titan syringe filter TITAN SYRINGE FILTER NYLON use for: • general laboratory filtration • filtration for most HPLC samples pore size pack size Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON Titan Syringe Filter, NYLON TITAN SYRINGE FILTER PTFE use for: • filtration of aggressive organic, highly basic or hot solutions • filtration of aqueous solutions after prewetting with an alcohol art.no. pore size pack size Titan...

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chromatography and spectroscopy - 6

titan syringe filter/target syringe filter TITAN SYRINGE FILTER RC use for: • low non-specific binding applications • tissue culture media and general biological sample filtration pore size pack size Titan Syringe Filter, RC Titan Syringe Filter, RC Titan Syringe Filter, RC Titan Syringe Filter, RC Titan Syringe Filter, RC TITAN SYRINGE FILTER CA use for: • tissue culture media filtration, sensitive biological samples art.no. pore size pack size Titan Syringe Filter, CA Titan Syringe Filter, CA Titan Syringe Filter, CA Titan Syringe Filter, CA Titan Syringe Filter, CA Titan Syringe Filter,...

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chromatography and spectroscopy - 7

target syringe filter 3. Target syringe filter TARGET SYRINGE FILTER PVDF use for: • general biological filtration • filtration where high protein recovery is desired pore size pack size PVDF Target Syringe Filter PVDF Target Syringe Filter TARGET SYRINGE FILTER RC use for: • low non-specific binding applications • Tissue culture media and general biological sample filtration pore size pack size RC Target Syringe Filter RC Target Syringe Filter TARGET SYRINGE FILTER AND TITAN SYRIN

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chromatography and spectroscopy - 8

solid phase extraction (spe) by macherey-nagel 4. Solid phase extraction (SPE) by Macherey-Nagel Solid-phase extraction (SPE) is a powerful method for sample preparation that is used today in most laboratories. The SPE shows its performance in a wide range of applications: environmental analysis, pharmaceutical analysis, organic chemistry and food analysis. SPE is used for three important tasks: enrichment of the analyte, removal of interfering components and change of the matrix of the analyte. Advantages of the SPE in comparison to classical liquid-liquid extraction: 1. Reduced...

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chromatography and spectroscopy - 9

thin layer chromatography (tlc) 5. Thin layer chromatography (TLC) The principle of TLC is known for over 100 years. TLC is still a popular technique in the laboratory for reaction, quality and identity control. Advantages of TLC are: • High sample throughput possible in a short time • No complex sample preparation required • Simple method without expensive instruments IMPLEMENTATION OF A TLC SEPARATION SELECTION OF THE SOLVENT: The solvent is selected by elution strength. The most appropriate solvent can be determined very easily. SAMPLE APPLICATION: A common method is to use a glass...

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chromatography and spectroscopy - 10

thin layer chromatography (tlc) TLC CONSUMABLES AND PLATES FROM MERCK art.no. TLC silica gel 60 Alu foil 20 x 20 cm TLC silica gel 60 F254 Alu foil 20 x 20 cm with fluorescent indicator TLC silica gel 60 F254 Alu foil 10 x 20 cm with fluorescent indicator TLC cellulose glass plate 20 x 20 cm UV-lamp 254 nm for thin layer chromatography TLC CONSUMABLES FROM MACHEREY-NAGEL art.no. TLC-developing chamber 10x10 cm TLC-developing chamber 20x20 cm Glas capillary, 1 μl Small rubber hats for capillaries Application template TLC PLATES FROM MACHEREY-NAGEL Alugram SilG: unmodified standard silica...

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chromatography and spectroscopy - 11

vials, micro inserts and closures for hplc 6. Vials, micro inserts and closures for HPLC THREAD BOTTLES ND8, NARROW OPENING MICRO INSERTS 1. hydrolytic class, 1.5 ml, standard bottles for HPLC/GC are preferably used on instruments of the following manufacturers: VWR (Merck)/Hitachi, Beckman, CTC, Gilson, Knauer, Shimadzu, Spark, Varian etc. 1,5 ml 32x11, 6 mm Clear glass Text box 1,5 ml 32x11, 6 mm Brown glass Text box 0,1 ml Micro insert 31x5 mm, Clear glass 15 mm tip 0,1 ml Micro insert 31x5 mm, Clear glass 9 mm tip 0,2 ml Micro insert 31x6 mm Clear glass with flat bottom 0,1 ml Micro...

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chromatography and spectroscopy - 12

vials, micro inserts and closures for hplc SHORT THREAD VIALS ND9, WIDE OPENING MICRO INSERTS 1. hydrolytic class, ND9 bottles are preferably used on instruments of the following manufactures: Agilent, Shimadzu, Thermo Scientific, Varian, Waters etc. 32x11,6 mm, Clear glass labelling field and filling mark 32x11,6 mm, Brown glass labelling field and filling mark 0,1 ml Micro insert 31x6 mm Clear glass 15 mm Tip 0,2 ml Micro insert 31x6 mm Clear glass Flat bottom 0,1 ml Micro insert 29x5,7 mm Clear glass assembled pastic spring POLYPROPYLENE SHORT THREAD VIAL CAPS ND9 PTFE virginal Natural...

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