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Guide to Capillaries, Reagents, and Supplies for CE and CEC

Guide to Capillaries, Reagents, and Supplies for CE and CEC
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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 1

Guide to Capillaries, Reagents, and Supplies for CE and CEC Maximize your CE and CEC System and experience greater laboratory products | applications | software | services Our measure is your success.

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 2

Your Link to Success – Agilent Technologies Capillary Electrophoresis Capillaries, Reagents, Solutions Kits, and Supplies Agilent Technologies, a world leader in capillary electrophoresis (CE) technology, is committed to helping you improve the quality of analyses by offering application solutions, simplifying the use of software and instrumentation, and offering unsurpassed technical support. Capillary Electrophoresis (CE) offers fast separations with exceptional efficiency and resolution for charged substances such as biomolecules, low molecular weight basic or acidic drugs and...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 3

Inorganic Anion Solutions Kit 7 Organic Acids Solutions Kit 9 Forensic Anions Solutions Kit 10 uPAGE Solutions Kits for High Resolution DNA Fragments Analysis 10 Standard Bare Fused-Silica Capillaries 12 Extended Light Path (Bubble Cell) Bare Fused-Silica Capillaries 13 Universal Bare Fused-Silica Capillaries 14 Polyvinyl Alcohol (PVA) Coated Capillaries_15 Cross-linked and Bonded uSIL Capillaries 17-18 Capillary Electrochromatography (CEC) Capillaries 19 Alignment Interfaces and Capillary Cassette 20 High Sensitivity Detection Cell 21 -22 CE System Start-up and Test Kits 26-27 Instrument...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 4

dissolved in water, diluted buffer or other minimally conductive Determine the analyte's charge using the following buffers: Check concentration by flow- injection analysis 1. use High Sensitivity Cell • If the sample contains high salt concentration desalt or increase the buffer

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 5

MEKC conditions Correct number of peaks? Is the peak shape good? Increase the sodium dodecyl sulfate concentration from 25 increments and/or decrease the Correct number of peaks? Is the peak shape good? modifier such as acetonitrile sensitivity optional? • Capillary length • Capillary inner diameter • Capillary voltage • Capillary temperature Determine method stability, Does the method meet the analysis criteria? Correct number of peaks? Is the peak shape good? Adjust variables buffer modifier such as Method completed

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 6

Agilent continues to introduce new CE solutions kits designed to simplify many of your applications: • Inorganic anions • Organic acids • Forensic anions • uPage kits for high resolution DNA fragment analysis These kits include all you need to begin your CE analyses, including buffers, capillaries, conditioning solutions, test samples, methods and detailed descriptions. Each kit is designed to take advantage of the automation of the Agilent CE system to make your time in the laboratory more efficient. All kits are prepared using the same quality procedures as our buffers and are thoroughly...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 7

Inorganic Anion Solutions Kit The Inorganic Anion Solutions Kit contains all components needed for the analysis of common inorganic anions such as chloride, bromide, iodide, fluoride, sulfate, and phosphate. Applications include the analysis • Ultra-pure water • Waste water • High purity chemicals • Drug formulations • Pulp and paper solutions • Semiconductor solutions Using an indirect-UV detection system optimized for small anions, analyses are sensitive and rapid, and provide an chromatography. The kit contains the buffer, capillaries, test mixture, and instructions. Separation of common...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 8

The Cation Solutions Kit provides everything you need for the analysis of inorganic and low-molecular-mass organic cations. It is specially designed for the separation of alkali metal ions, alkaline-earth metal ions and alkyl amines in a wide range of different matrices. Each kit contains a cation buffer, bare fused silica capillaries, a cation standard, CE grade water and a detailed description of the analysis method and most common applications, including detection limits and reproducibility data. The Cation Solutions Kit and the separation methods were developed to fit perfectly with the...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 9

Organic Acids Solutions Kit The Organic Acids Solution Kit is ideal for the analysis of short alkylchain carboxylic acids. Employing an indirect UV detection agent optimized for organix acids, the methodology is simple, sensitive, and provides accurate quantitative analysis. Suited for the analysis of organic acids in a wide range of matrices, it is especially useful for determination of organic acids Organic acids in beer and red wine Organic Acids Solutions Kit Organic Acids Solution Kit Organic acids buffer Ultra pure CE water 3are fused-silica capillary, 75 urn ID, 72 cm long Organic...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 10

Forensic Anions Solutions Kit This highly focused kit was developed specifically for the analysis of poisonous compounds, such as cyanide, azide, selenate, arsenate, and arsenite. In cases of poisoning, analytical tools are needed to determine teh identity of the toxins quickly and accurately. A rapid determination of anionic toxins in adulterated foods and beverages is possible using CE with indirect UV detection. The forensic and other anions can be detected within 15 minutes with minimal sample preparation. Analysis of an Anion standard with the Forensic Aniona Analysis Kit Forensic...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 11

MPAGE capillaries are available in three different pore sizes. The size of the molecular sieving pores is controlled by degree of polymer cross-linking (% C). Gels with higher %T and %C values have smaller pores and are, therefore, more effective at resolving smaller smaller molecules uPAGE- 10(10 % T, 0 % C) capillaries provide high resolution capabilities for separation of antisense therapeutic agents, primers and probes as well as nucleotides. uPAGE-5 (5 %T, 5 %C) allows single base ranging from 20-150 bases. For your convenience, uPAGE capillaries and uPAGE buffers can be purchased...

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Guide to Capillaries, Reagents, and Supplies for CE and CEC - 12

Standard Bare Fused-Silica Capillaries Fused-silica capillaries are the heart of CE. Pre-aligned capillaries from Agilent Technologies are designed and optimized for ease of use and reliability. All capillary ends are cut to a smooth, mirror-like finish. In addition, the polyimide outer-coating is removed from the ends. These processes ensure minimal sample adsorption and help maintain sharp peak shapes. All capillaries have a pre-made detection "window" and a built-in alignment stopper which allows rapid and precise insertion in the alignment CZE of a tryptic digest of recombinant human...

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