Integrated Biology Solutions AT A G L A N C E Agilent Technologies has developed and optimized products and solutions to address an entire range of protein identification challenges. Let our comprehensive line of protein identification tools put you on the road to faster discovery and identification of lowabundance proteins within complex samples. LC/MS solutions to discover and identify more proteins from complex samples

A comprehensive and integrated workflow for fast and accurate protein identification The identification of proteins from biological samples presents unique challenges. A single sample can contain thousands of proteins whose abundances vary over many orders of magnitude. Often, the proteins of most interest are present at lower abundance. Agilent Technologies offers an integrated suite of powerful tools that enables you to successfully prepare, separate, analyze, and identify your challenging protein samples. Optimized preparation products, outstanding LC separation technologies, exceptionally...

Protein Identification Solutions from Agilent Sample preparation technologies that optimize analytical performance By combining our proprietary reagents and consumables with our instrument systems, you can take advantage of enhanced performance, reduced time- to-results, and an improved, easier We have solutions for all stages of your sample preparation—from the removal of interfering, high-abundance serum proteins to in-gel tryptic digestion kits for effective and reproducible results. Agilent's efficient sample preparation technologies improve the consistency and reliability of your results....

HPLC-Chip/MS and Nanoflow-LC systems for superior sample separations No single separation technique is suitable for all protein identification studies. Some prefer the remarkable performance and ease of microfluidic chip-based separations. Others need the flexibility of nanoflow LC. That’s why Agilent offers multiple separation approaches for protein identification—so every lab can find the perfect, custom-tailored fit to identify more proteins faster and with greater confidence. The HPLC-Chip Cube interface Conventional nanoflow LC uses numerous intricate connections and requires substantial...

Protein Identification Solutions from Agilent The Nanoflow-LC System for MS If you have a diverse set of sample types or experimental objectives, you’ll want to consider various separation approaches. The flexibility of Agilent’s Nanoflow-LC system allows you to design a variety of separation workflows so you can apply the methods that work best in your lab and achieve exactly the separations you want. The Nanoflow-LC system can be configured for onedimensional separation, onedimensional separation with sample cleanup and enrichment, or full twodimensional separation. High-efficiency columns...

Exceptional MSn performance for protein sample analysis The identification of low-abundance proteins requires exceptional sensitivity and specificity. However, these requirements can certainly be met in different ways. You may prefer the convenience of online integration of microfluidic nanoflow-LC and a nanospray ion source. Or you may desire the efficiency of high-throughput analyses provided by MALDI-based MS. Perhaps another particular combination of separation and MS approaches would best suit your needs. With these possibilities in mind, Agilent provides multiple approaches to help you...

Protein Identification Solutions from Agilent LC/MSD Trap XCT Ultra ion trap MS The LC/MSD Trap XCT Ultra consistently matches or exceeds the sensitivity and scan speed of other three-dimensional ion traps and two-dimensional, linear ion traps. For optimal sensitivity, the XCT Ultra combines a multipole design that increases trap capacity and a Smart Target function that maximizes trap capacity usage. At the same time, it retains the resolution and scan speed advantages inherent in a true three-dimensional ion trap. Faster scanning helps identify more peptides Advanced capabilities for easier,...

Powerful software tools identify more proteins quickly High quality data is necessary, but not sufficient. It takes the right software tools to turn good data into useful, relevant informa- tion. Agilent offers the Spectrum Mill MS proteomics workbench, a comprehensive suite of software tools that turn your MSn data into answers. The Spectrum Mill workbench provides • Intelligent spectral extraction and quality assessment • Database searching and de novo • Automated and interactive results • Cross-experiment result summaries • Multiple options including MS/MS search for MS/MS spectra and peptide...

Protein Identification Solutions from Agilent Quantitative as well as qualitative The Spectrum Mill workbench can com- pare relative protein abundances based on mean peak intensities of all the com- ponent peptides from a protein; this method can reveal two- to five-fold changes in relative abundances. When you need more precise quantitation, the Spectrum Mill workbench fully supports stable isotope and other labeling experi- De novo spectral interpretation for peptides not found in any database The de novo sequencing algorithm uses advanced graph theory to generate a ranked list of potential...

Protein identification solutions from Agilent Our protein identification solutions combine Agilent’s advanced tools for protein analysis into integrated solutions for analyzing complex mixtures of proteolytically digested proteins. Whether you require convenient, flexible, or high-throughput analyses, we provide a direct solution that delivers high performance. Agilent HPLC-Chip/MS protein identification solution for performance and ease Agilent Nanoflow proteomics solution for flexibility Agilent MALDI proteomics solution for high-throughput analysis Identify more proteins more quickly and with...

Protein Identification Solutions from Agilent Protein ID solution products Spin Filters Multiple Affinity Removal Systems* Spin Concentrators Protein In-gel Tryptic Digestion kit Peptide Cleanup C18 spin tubes and pipette tips MALDI-MS Matrices Peptide and Protein standards HPLC-Chip Cube MS interface Agilent 1100 Series Nanoflow LC System for MS Autosampler cooling Agilent orthogonal nanospray source Agilent PDF-MALDI source Agilent LC/MSD Trap XCT Ultra Micro fraction collector/plate spotter Spectrum Mill MS Proteomics Workbench Protocols for sample preparation and MS analysis Familiarization/troubleshooting...