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3100 fractionator

3100 fractionator
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$3100 fractionator - 1$

proteins and peptides with liquid-phase recovery Agilent Technologies

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Achieve unprecedented sensitivity in LC/MS-based proteomics experiments Sample complexity and large dynamic range of protein and peptide concentrations are major hurdles in the analysis of low abundance proteins. Today, for almost any proteomic analysis, robust and reproducible fractionation techniques are key to ensure that MS/MS can identify the maximum number of proteins. At the same time, to avoid inadvertent loss or alteration of critical proteins, it is important that fractionation steps minimize the number of sample manipulations. OFFGEL electrophoresis separates proteins or peptides...

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$3100 fractionator - 3$

Easy integration into any protein analysis workflow Whether your task is biomarker discovery, protein identification or purification of functional proteins or peptides, the Agilent 3100 OFFGEL Fractionator fits into any protein analysis workflow. Integration of the OFFGEL system is easy and ultimately increases sensitivity of subsequent OFFGEL Fractionator • pl-based OFFGEL fractionation with liquid-phase recovery for easy transfer to downstream techniques • Reproducible fractionation with downstream techniques such gel-based analysis for maximum • pi values obtained act as additional...

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OFFGEL electrophoresis – high resolution pI fractionation based on proven IPG technology The Agilent 3100 OFFGEL Fractionator performs isoelectric focusing of proteins or peptides in immobilized pH gradient (IPG) gel strips. The technique applied by the 3100 OFFGEL Fractionator differs from conventional gel electrophoresis in that sample components are recovered from liquid phase. This makes the technology directly compatible with liquid-phase workflows, for example LC-MS. a) The IPG gel strip is rehydrated and seals tightly against the well frame. b) The diluted sample is equally...

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Individual sample diagnostics for a quick and easy check of fractionation quality With its state-of-the-art user interface the 3100 OFFGEL Fractionator measures and records voltage, power and current during fractionation in OFFGEL and traditional IEF mode. Currents of each sample are measured and displayed online individually. Current and voltage information can be visualized using the online plot or by exporting the data to a spreadsheet application, providing direct diagnostics for the quality of each individual sample fractionation. Five easy steps to set up and run a fractionation 1....

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Protein Fractionation Achieve highest resolution The Agilent 3100 OFFGEL Fractionator provides pl-based fractionation of peptides or proteins with exceptional resolution. Samples can be fractionated into 12 or 24 fractions for standard Obtain greater reproducibility Fractionation by OFFGEL electrophoresis is highly reproducible. Fractions from several runs may be pooled for enrichment of low abundance proteins Fractionation of E.coli into 24 fractions (pH 4-7). Analysis of fractions 9 and 12 by 2D electrophoresis. The theoretical resolution of 0.13 pH units is indicated by the red bars....

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Peptide Fractionation Gain sensitivity Efficient reduction of sample complexity increases sensitivity of downstream analysis. This example compares the efficiency of fractionation of the tryptic peptides of immunodepleted plasma by strong cation exchange (SCX) with OFFGEL electrophoresis. Immunodepletion by multiple affinity removal Digestion of depleted plasma with trypsin OFFGEL electrophoresis strong cation exchange Peptide fractionation by OFFGEL electrophoresis (pH 3-10, 24 fractions) is more efficient than separation by SCX (50 fractions) as indicated by the 3-fold higher number of...

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$3100 fractionator - 8$

Agilent 3100 OFFGEL Fractionator Instrument specifications Type Weight Dimensions (height × width × depth) Line voltage Line frequency Power consumption Ambient operating temperature Humidity Operating altitude Safety standards IEC, CSA, UL 100 – 240 VAC, ±10% 50 – 60 Hz, ±5% 140 VA 5 – 40 ºC < 80% at 40° C (104 °F) Up to 2000 m (6500 ft) Installation Category II, Pollution Degree 2 10 ºC – 35 ºC Platform temperature Power supplies Fractionation capacity Two independent high voltage power supplies Voltage-Range: 0.5 – 10 kV Current-Range: 0 – 150 µA/strip Power: 0 – 1 W/strip Modes:...

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