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SB200-X

Product catalog summary

Overview
The SB200-X is an orbital shaken bioreactor designed for cultivating human, mammalian, and plant cells in disposable bags. It supports a working volume of 50 to 200 liters and is suitable for production environments due to its user-friendly setup and scalability.

Performance Characteristics

Large capacity with a 200L working volume.
Supports cultivation of various cell types.
Fast and simple setup with easy scale-up capabilities.
Online measurement of pH and dissolved oxygen (DO).

Disposable Bag System
The use of disposable bags eliminates the need for complex cleaning and sterilization, reducing setup time and costs. The bags are designed without additional mixing devices, minimizing mechanical damage risks and enhancing process security.

Orbital Motion
The SB200-X employs orbital motion for efficient liquid mixing, ensuring high oxygen transfer rates with low shear forces. This method reduces mechanical stress on cells and is suitable for microcarrier cultivations.

Scale-Up and Control
The system allows easy scale-up from microtiter plates to 200 liters, thanks to consistent hydrodynamics. It features a reliable Kuhner direct drive for precise orbital speed control. The control unit includes touch screen technology and ISIS 8.0 software for data recording.

Gas Mixing
The integrated FlowCon gas mixing device stabilizes pH and maintains oxygen levels. It includes four mass flow controllers for accurate gas mixing and an integrated pressure sensor to prevent overpressure.

Technical Specifications

Shaker speed: max 80 rpm with a 50 mm orbital motion.
Temperature range: up to 50°C with cooling options.
Oxygen sensor accuracy: ±0.01%O2 at 0.21%O2.
pH sensor range: 5.5 – 8.5 with high accuracy.
Power supply options: various voltage ranges available.

Software and Compliance
The ISIS-Software facilitates monitoring, calibration, and control, supporting GMP compliance with 21 CFR Part 11. It offers interfaces like CAN-bus, CAN-USB, and CAN-Ethernet.

Accessories and Options
Additional options include a cooling system and IQ-OQ documentation for GMP compliance. The disposable bag features multiple ports for various processes and is composed of three layers for durability and chemical resistance.

Contact Information
Adolf Kühner AG, Dinkelbergstrasse 1, CH – 4127 Birsfelden (Basel), Switzerland. Phone: +41 (0) 61 319 93 93, Email: [email protected], Website: www.kuhner.com

Catalog excerpts

Kuhner shaker Orbital shaken bioreactor

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The SB200-X is a shaken bioreactor system for the cultivation of human, mammalian and plant cells in a disposable bag. This user friendly system with a working volume from 50 litres up to 200 litres can be easily deployed in production. Performance characteristics + Large capacity (200L working volume) + + + + single use bioreactor system Cultivation of human, mammalian and plant cells Fast, simple set up Easy scale-up Online measurement of pH and DO Disposable bag for a fast and simple set up The use of disposable bags eliminates elaborate cleaning and sterilizing procedures and ensures quick...

Open the catalog to page 2

Trouble free scale-up Another advantage of the SB200-X is easy scale-up from microtiter plates all the way up to 200 litres. The reason for this lies in the consistent hydro-dynamics of shaken bioreactors. The SB200-X uses the unique Kuhner direct drive which provides accurate control of the orbital speed. The machine`s mechanical design has a proven history of reliability. Control unit and process safety The SB200-X can be heated or cooled. Temperature ramps are easily programmed and processed. Duplex online measurement of each parameter (dissolved oxygen and pH value) is available ensuring...

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Technical data Shaker diameter 50 mm (orbital motion) Weight approx. 350 kg without liquid Active brake adjustable Cooling cooling coils are incorporated for connection to an external cooling system (pressure <0.2bar) • Oxygen Sensor Optical measuring method Accuracy ± 0.05 pH at pH 7 with one point calibration • Mains connection

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• ISIS-Software for monitoring, calibration, controlling Monitoring 02, pH, shaking speed, temperature Calibration pre-calibrated sensors in bag Recalibration one point recalibration is possible Ci.V.P 21 CFR Part 11 compliance (audit trail) Number of mass flow controllers up to 4 Input up to 4 gases, push in connector 6 mm (air, oxygen, nitrogen, carbon dioxide) (also gas mixtures can be connected) Output 1, (tube fitting for tube ID 13 mm) Flow rate 0.0 - 20.0 [sL/min] Accuracy ±1.5% from the end value Pressure sensor integrated, 0-400 mbar, programmable prevents over pressure in the connected...

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Accessories Disposable bag Order number: SMX710001 The use of disposable bags eliminates elaborate cleaning and sterilising procedures and ensures quick set up times. The bag for the Kuhner shaken bioreactor is less complicated and therefore less expensive than others because no additional mixing device is required (e.g. stirrer, ...). This also reduces the risk of mechanical damage, improving process security. The single-use bag has a working volume from 50 litres up to 200 litres. Various ports are available for feeding, inoculation, harvesting and sampling. The bag also incorporates sterilized...

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Options Cooling The SB200-X has a cooling ring embedded in the base plate and cooling is achieved by connecting an external cooling device. An integrated valve, controlled by the ISIS-Software, regulates the set point temperature. IQ-OQ Documentation IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification required for GMP procedures. This service is available from Kuhner and can also be carried out at the customer`s premises.

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