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RAMOS

Product catalog summary

Overview: The RAMOS (Respiration Activity Monitoring System) is designed for online determination of oxygen transfer rate (OTR) and carbon dioxide transfer rate (CTR) in microbial, plant, and cell cultures. It supports up to 8 parallel fermentations and is applicable for various shaking machines, including SBM/SS-X, LT-X, ISF1-X, and ISF4-X. The system automates measurements, reducing optimization time and manual labor.

Specifications:

Dimensions: 800x550x490 mm (including incubator, excluding PC)
Weight: Approximately 66 kg with incubator and shaker
Shaker tray size: Approximately 420x420 mm
Power requirement: 230V / 3A or 110V
Temperature range: Room temperature + 5°C to 50°C
Temperature regulation consistency: ±0.05°C
Spatial deviation: +0.3°/-0.7°C
Shaker speed: Maximum 300 rpm
Shaking diameter: 12.5/25/50 mm
Flasks: 8 measurement flasks (250 mL) with optional 500mL or 100 mL

Measurement Capabilities:

Pressure sensors: Measurement uncertainty ≤ ±5%, reproducibility ≤ ±1%
Oxygen sensors: Measurement uncertainty ≤ ±5%, reproducibility ≤ ±2%
Gas flow: Measurement uncertainty ≤ ±5%, reproducibility ≤ ±3%
Sensitivity for OTR and CTR: Calculated based on flask and culture volume
Respiratory Quotient (RQ): ± 0.1

Applications: RAMOS is suitable for various biological phenomena and meets the FDA's PAT initiative for shaken bioreactors. It is particularly useful for optimizing fermentation conditions for organisms like Pichia stipitis and Corynebacterium glutamicum under specified conditions.

Contact Information: Adolf Kühner AG, Dinkelbergstrasse 1, CH – 4127 Birsfelden (Basel), Switzerland. Phone: +41 (0) 61 319 93 93, Email: [email protected], Website: www.kuhner.com

Catalog excerpts

RAMOS Respiration Activity Monitoring System for online determination of OTR and CTR

Open the catalog to page 1

Applicable for the following shaking machines SBM/SS-X, LT-X, ISF1-X, ISF4-X 2 Selection of typical biological phenomena RAMOS RAMOS (Respiration Activity Monitoring System) determines the oxygen transfer rate (OTR), the carbon dioxide transfer rate (CTR) and the respiratory quotient (RQ) of microbial, plant and cell cultures online. The respiration rates (OTR, CTR) are the most suitable measurable variables to quantify the physiological state of those cultures. + 8 parallel fermentations + One system for microbial, + + + + plant and cell cultures Very easy handling Automated system Reduces optimization...

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Pichia stipitis Complex medium with 20g/L glucose, temperature 30°C, shaking freguency 100 rpm, shaking diameter 50mm, , oxygen transfer rate ~m- carbon dioxide transfer rate Corynebacterium glutamicum Mineral medium with 30 g/L glucose, temperature 30°C with CaC03 shaking freguency 200 rpm, app. 66 kg with incubator and shaker • Size of shaker tray • Power requirement • Temperature range Time consistency • Spatial deviation only by operating with gas mixtures differing from surrounding air • Shaker speed Flasks • Measurement uncert. + 6 extra shaker flasks • Reproducibility (sample flasks, not...

Open the catalog to page 3