FeedBeads® Polymer based slow release system for glucose

Release Kinetic of the ø 6mm FeedBeads Conditions: 0.1M phosphate buffer, pH = 7, T = 37°C 2 Release Kinetic of the ø 12mm FeedBeads Conditions: 0.1M phosphate buffer, pH = 7, T = 37°C FeedBeads® Controlled glucose delivery by slow release technology FeedBeads provide substrate limited fed-batch conditions in the shaken bioreactor without the need for enzymes or additional equipment such as tubing or pumps. + Improves screening security + Suitable for high throughput + + + + + + screening (HTS) Easy handling Fed-batch without tubing and pumps Reduces overflow mechanism of the culture Reproducible...

YFP-Production with recombinant E. coli Mineral medium; temperature 37°C; shaking diameter 50mm; shaker speed 350 rpm; VL = 12.5 mL FeedBeads® | 3 Fed-Batch preculture of E.coli BL21 YFP in MTP Wilms-MOPS medium without carbon source; temperature 37°C; shaking diameter 50mm; shaker speed 400 rpm; VL = 700 µL Product information 25 pieces, Ø12mm (shake flasks), 100 pieces, Ø 6mm (microtiter plate) • Substrate glucose on request other crystalline nutrients are available as the limited substrate Order information: SMFB63319: FeedBeads Glucose (Ø 12mm), pack of 25 Publication: Fed-batch mode in shake...

Fed-Batch without tubing & pumps www.kuhner.com Adolf Kühner AG • since 1949 Dinkelbergstrasse 1 CH – 4127 Birsfelden ( Basel ) Switzerland