Cell Culture

Cell Culture
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Cell Culture

Product catalog summary
Introduction
ACE GLASS Incorporated has been a leader in glass labware and scientific equipment for over seventy years. The 2014 Cell Culture Products Catalog introduces products specifically for cell culture labs, made from borosilicate glass meeting USP and ASTM Type 1, Class A specifications.
Product Specifications
  • Bottle Solution, Plastic Coated: Features a plastic safety coating to contain particles and chemicals if broken. Maximum temperature limit is 80°C, with higher temperature coatings available on special order.
  • Cloning Cylinder: Used for isolating cells in culture flasks, available in various dimensions.
  • Filling Bell Aseptic: Designed for aseptic filling of vessels, compatible with rubber tubing.
  • Flask Culture, Fernbach: Available in various designs for culturing organisms requiring a large surface-area-to-volume ratio.
  • Flask Shaker: Designed with baffles to enhance gas transfer, available in various capacities and neck styles.
  • Flask Trypsinizing: Used for converting tissue samples into cell suspensions, featuring beaded necks and baffles for agitation.
  • Flask Media Storage and Dispensing: Designed for sterile dispensing with a bottom hose connection.
  • Tube Gas Dispersion: Available in various porosities and lengths for fine dispersion applications.
Key Features and Recommendations
  • ACE GLASS products are known for their quality and performance, suitable for various laboratory applications.
  • Products are designed to enhance safety and efficiency in laboratory environments.
  • Customization options are available for specific laboratory needs.
Conclusion
ACE GLASS continues to innovate and provide high-quality glassware solutions for scientific research, focusing on cell culture laboratories.
Filtration Apparatus Overview
Ace Glass introduces innovative lab filtration apparatus using the Ace-Thred design, eliminating the need for silicone stoppers and clamping devices, thus preventing leakage. The apparatus consists of a funnel, flask, nylon adapter, and a removable glass fritted disk, allowing for easy cleaning and the use of different frit sizes. The assembly is autoclavable and available in two sizes: 25mm and 47mm.
Specifications and Components
  • 25mm Filtration Apparatus: Includes a 500mL filter flask, nylon adapter, 100mL graduated funnel, and a 25mm Porosity B fritted disk. Priced at $323.50.
  • 47mm Filtration Apparatus: Includes a 1000mL filter flask, nylon adapter, 500mL graduated funnel, and a 47mm Porosity B fritted disk. Priced at $412.27.
Tissue Homogenizers
  • Tapered Glass Homogenizer: Features two grinding surfaces for initial and final homogenization, available in capacities from 2mL to 50mL.
  • Dounce Homogenizer: Comes with two pestles for cell dissociation, available in capacities from 1mL to 40mL.
  • Potter-Elvehjem Homogenizer: Features a PTFE pestle and is available in standard and micro sizes, with capacities ranging from 0.1mL to 55mL.
  • Tenbroeck Homogenizer: All-glass design with interchangeable pestles, suitable for tissues like liver and heart, available in capacities from 1mL to 40mL.
Key Features
  • Removable frits for easy cleaning and different porosity options.
  • Improved membrane utilization with larger effective filtration area.
  • Customizable adapters available in PTFE or other polymers upon request.
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Catalog excerpts

Cell Culture-1

Cell Culture Products LABORATORY GLASSWARE

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Cell Culture-2

2 ACE GLASS Incorporated, a leader in the design and manufacture of glass labware and scientific equipment, has been supplying research laboratories with quality products for over seventy years. Since 1936, ACE products have been a part of labs performing analytical functions, as well as life sciences labs. In this new Cell Culture Products Catalog, ACE GLASS presents a line of products for cell culture labs and offer glass lab products designed specifically for use by the cell culturist. ACE laboratory glass products are made from borosilicate glass and meet USP and ASTM Type 1, Class A specifications,...

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Cell Culture-3

3 FLASK Culture, Fernbach, Triple Baffled, Beaded Neck   Wide mouth flask designed for culturing organisms requiring a large surface-area-to-volume ratio. Triple baffled on the bottom edges to achieve maximal oxygen transfer to culture medium. Beaded neck is 63mm I.D. and accepts cotton plugs or rubber stoppers. Caution! Do not place on direct heat source. Capacity, mL 1800 2800 FLASK Culture, Fernbach, Baffled, Plain Neck, without Closure   With a long-style 38mm neck for plastic or Stainless Steel closures. Triple baffles are located at the center of the bottom to achieve maximal oxygen transfer...

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Cell Culture-4

4 FLASK Shaker, with Three Side Baffles, without Closures   Long-style neck with three side baffles to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures. Flask, only, 50 mL Flask, only, 125 mL Flask, only, 250 mL Flask, only, 300 mL Flask, only, 500 mL Flask, only, 1000 mL Flask, only, 2000 mL FLASK Shaker, without Closure   With long-style neck for use with plastic closures. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Capacity, mL 25 50 125 250 300 500 1000 2000 4000 6000 FLASK...

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Cell Culture-5

5 FLASK Shaker, Deep Baffles, with Beaded Neck   Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Approx. O.D. x Height, mm   85 x 130   90 x 140 100 x 170 130 x 215 160 x 285 Flask, only, 250 mL Flask, only, 300 mL Flask, only, 500 mL Flask, only, 1000 mL Flask, only, 2000 mL FLASK Shaker, with Three Side and Three Bottom Baffles,   without Closures Similar to 3887, except these long-style neck flasks have three extra-deep baffles on side and bottom to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces...

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Cell Culture-6

6 FLASK Trypsinizing, with Sidearm, Screw Caps, Baffled   Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Phenolic screw cap with rubber liner on neck and sidearm. Designed with well below the sidearm to prevent unseparated tissues from being poured out of the flask. Three extra-long, extra-deep baffles promote vigorous agitation. Capacity, mL 75 150 300 FLASK Media Storage and Dispensing, Bottom Hose   Used for sterile dispensing; use with an aseptic filling bell. Bottom ½‑inch hose connection assures aseptic drainage. With beaded rim....

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Cell Culture-7

7 FLASK Nephelo Culture, Triple Baffled, without Closures   Long-style neck accommodates a 25mm Stainless Steel or plastic closure. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Has standard cuvette size side arm. Capacity, mL 300 300 300 FLASK Nephelo Culture, Triple Baffled, with Screw Cap   Threaded neck with cleanout port and depressed sidearm. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Supplied with GPI #38-415 and GPI #18-415 nontoxic, rubber-lined phenolic screw caps. Case/4. Capacity, mL 300 500 300 500 FLASK Filtering, Heavy Wall,...

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Cell Culture-8

Outside diameter of tube 8mm, total length of tube 150mm. All porosities of a given size are ACE Maximum Pore Dia. Corning Kimble Designation Range (micron) Equivalent Equivalent Most Frequent Uses A 145-174 EC (170-220) EC (170-220) Coarse filtration. Gas Dispersion B 70-100 Coarse filtration. Gas Dispersion VF 2-2.5 VF (2-2.5) General bacteria filtrations UF 0.9-1.4 UF (0.9-1.4) General bacteria filtrations

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Cell Culture-9

9 TUBE Gas Dispersion   For fine dispersion. Porous tip approximately 5mm or 7mm O.D. x 10mm length. 7mm O.D. tube fits #7 Ace-Thred. Stem O.D. x Overall Length, mm 5 x 135 7 x 135 5 x 210 7 x 210 Por. A Order Code 9435-06 9435-21 9435-36 9435-51 Stem O.D. x Overall Length, mm 5 x 135 7 x 135 5 x 210 7 x 210 Por. B Order Code 9435-07 9435-22 9435-37 9435-52 Por. D Order Code 9435-09 9435-24 9435-39 9435-54 Por. C Order Code 9435-08 9435-23 9435-38 9435-53 Por. E Order Code 9435-10 9435-25 9435-40 9435-55 TUBE Gas Dispersion   With porous fritted glass 10 x 30 mm tip. Available in 150mm, 300mm,...

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Cell Culture-10

Using Ace-Thred design, filter funnel and filter flask thread together without the silicone stopper and troublesome clamping device. Nylon membrane retaining ring completes seal. Nylon adapters standard, PTFE or other polymers. Better membrane utilization! uses 45mm of the 47mm grid vs. only 35-40mm in older Removable frits! Unit disassembles completely for easy cleaning. Uses removable frits of different porosities, from extra coarse to extra fine, instead of expensive, sealed-in frits. Ace Glass introduces a new concept for lab filtration apparatus. Using our famous Ace-Thred design, we are...

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Cell Culture-11

11 TISSUE HOMOGENIZER Tapered, Glass, Interchangeable   Highly efficient, all glass, tapered tissue grinder combining two grinding surfaces on a single pestle and tube. The conical area is for initial grinding and the cylindrical area for final homogenization. Interchangeably ground to 0.1–0.15mm clearance. With pouring lip. Capacities represent volumes with pestle inserted. Cap., mL  2 15 30 50 Tube, only Order Code 8325-04 8325-16 8325-22 8325-28 Pestle, only Order Code 8325-06 8325-18 8325-24 8325-30 TISSUE HOMOGENIZER Dounce, Two Glass Pestles   Supplied with two pestles, one “loose” and...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.