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Cryopreservation without crosscontamination risk

Cryopreservation without crosscontamination risk
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Cryopreservation without crosscontamination risk - 1

Cryopreservation without crosscontamination risk Biological materials such as cells or tissue samples will naturally change and deteriorate over time. In order to properly preserve them for later study, some means of halting these processes must be used that will not change the biological materials. The most effective means of preserving biological materials is cryopreservation – storage at extremely low temperatures. Cryopreservation has been used for decades throughout the biological and bio-medical research community. he most important element of a lowtemperature storage system is ensuring a constant range of temperatures below a minimum critical threshold. The upper limit of the range should be well below the critical temperature for the material to allow for any effects or compromise during stocking and retrieval activities. For example, the critical temperature for living cells is below -130ºC; therefore, maintaining cells in a liquid nitrogen freezer at -150ºC to -196ºC is ideal. Panasonic Biomedical Sales Europe B.V. has established a worldwide reputation as a manufacturer of high quality medical equipment over the past forty years. In this period we became the leader in the European Ultra Low Temperature market. In this field Panasonic has set the standard in many aspects. VIP panels, Cool Safe® compressors and the world’s first -152ºC ULT freezer. Where Panasonic took the initiative, the others followed. Together with the high standard of service we deliver it makes us a major player in the biomedical market. refrigeration system enhances reliability of long-term preservation. With a powerful low noise design afforded by traditional ultra-low temperature technology, this freezer delivers durable, stable cooling. Why freeze to -152ºC? Recrystallization mechanism Cubic crystal Compressors continuously repeat highly compressed operations, so lubricant oil is essential to prevent abrasion and seizure. But when lubricant oil circulates in the refrigeration circuit, piping becomes clogged and results in compressor damage. Incorporating a high-efficiency oil separator, Panasonic -150ºC freezers effectively separate lubricant oil from refrigerant, offering a stable ultra-low temperature environment. Highly efficient compressors have been specially developed and incorporated in -150ºC freezing units. A refrigeration circuit with the Cool Safe® compressor cascade Panasonic Biomedical Journal Hexagonal crystal

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Cryopreservation without crosscontamination risk - 2

Panasonic Biomedical Journal

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Cryopreservation without crosscontamination risk - 3

Liquid nitrogen is like all liquids, a possible transporter of contaminants. Transmission of biological contaminants between samples and possible leakage of liquid nitrogen into the vials when submerged are just some of the concerns that can be eliminated with an Isothermal liquid nitrogen dry storage freezer from Custom BioGenic Systems (CBS). When in the 1980’s HIV/AIDS was first recognized, storage in liquid became an increasing concern. These concerns have been scientifically demonstrated and are documented in papers such as from Teddar et al.,1995, Fountain et al.,1997 and Bielanski et...

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Cryopreservation without crosscontamination risk - 4

With a minimal temperature gradient, the full internal volume of each freezer is available for storing samples at liquid nitrogen temperatures making the most efficient use of available capacity. CBS Isothermal Freezers also provide added user safety by eliminating contact or splashing of liquid nitrogen. • Medical Device Directive 93/42/EEC: 1993 Class IIA. • Choice of 8 models offering capacities between 9,100 and 45,000 2ml vials (maximum). • All models feature the Series 2301 Auto-fill and Monitor System providing sample security and ease of operation. • A wide selection of inventory...

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