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Agilent GC-MSD and QTOF

Agilent GC-MSD and QTOF
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Vorstellung GC-QTOF

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Agilent GC/MS Portfolio Agilen! Technologies

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Agilent GC/MS Portfolio

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Agilent GC/MS Portfolio

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More Choices - Better Solutions Agilent GC/MS & GC/MS/MS

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Classes of chemicals and the analytical techniques with which they are most compatible GC Alkylsilyl derivatives Eicosanoids Essential oils Esters Perfumes Terpenes Waxes Volatiles Caratenoids Flavenoids Lipids LC Alcohols Alkaloids Amino acids Catecholamines Fatty acids Phenolics Polar organics Prostaglandins Steroids Organic Acids Organic Amines Nucleosides Ionic Species Nucleotides Polyamines overlap Less Polar More Polar

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Different Sample Introduction Systems Liquid Insertion Headzpace Thermal Separation Probe

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Capillary Flow Teclinoloj "Quick Swap" Solvent Venting Heart Cutting (Deans Switch) Split to several Detectors

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Electron Impact lonization (El) 'Hard' ionization technique Extensive Fragmentation Limited molecular ion - molecular weight Structural information High Vacuum (10-5 torr) To limit reactions between molecules Very reproducible

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Compounds must be able to accept a proton Amines, alcohols Proton transfer reaction

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egative Chemical lonization (NCI) Compounds must be able to accept an electron Electronegative atoms - halogens (F, Cl, Br, I) Conjugated Systems - phenyl rings Electron capture reaction

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New Agilent 5977 Series GC/MSD The 5977 is the new Agilent GC/MSD platform and replaces the • Improves Performance with high sensitivity Extractor Ion • MassHunter GC/MS Acquisition • MassHunter and MSD ChemStation data analysis • Integration of new Agilent Vacuum Pumps brings intelligent • Direct Communication between 7890B and 5977Afor more

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New Agilent 5977 Series GC/MSD S Proven core technology since 5973/5975 • Heatable Gold Quartz Quadrupole • Triple Axis Detector • High Energy Diode • Triple Channel EM Detector • Productivity Software (MSD ChemStation Data Analysis)

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New Agilent 5977 Series GC/MSD • High Sensitive Extractor Ion Source • Improved Agilent Vacuum Pump • Protect System • Vent the System faster • Power save (Sleep/Wake) — • MassHunter Software * • Data Acquisition (Method/Sequence) • Data Analysis

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and improved thermal profile

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New Extractor Ion Source for 5977 Why the Extractor EI Source? Increase in ion count True increase in sensitivity Better trace level precision Lower IDL and MDL Page 16

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Comparison betwe^n-hrert Ion Source and Extractor loruSötirce Repeller lonization area Drawout Plate Extractor Lens Entrance Lens Mass Filter Mass Filter

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Föw Extractor Ion source with "Etune" be seen in Blue - Extractor 40ppb Solution (SVOC) Red - Standard 40ppb Solution (SVOC)

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More improvements on 5977's Extractor Ion Source Improved TheiHTfal Profile Better Performance of high boilers i.e. PAH even at lower concentration! Extractor Source Response of high boiling 5ppb PAH declining over sequential IQ Injections Improvement for better Mass

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Better Specs for Working Methods: Instrument Detection Limit (IDL) More ions = Better precision = Better IDL = Better MDL 10 fg Extractor EI (24 fg Inert EI/TP) Method conditions TESTED at INSTALLATION Agilent #1 This is NOT just a factory spec, but actual demonstration in Worldwide (need ALS for demonstration) Page 20

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ASK How is the sensitivity with 5977? ANSWER Highest Signal-to-Noise Lowest Instrument Detection Limit 21

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Sensitivity Specifications of the 5977 Full Scan EI Specification: S/N - Signal-to-Noise The 5977 in scan mode has some background noise even if only OFN standard is injected, therefore the traditional Signal-to-Noise (S/N) is used as specification. Agilent’s 5977 has the highest S/N (1500:1) with Extractor Ion Source among the SQ system. (1pg OFN) SIM Specification: IDL - Instrument Detection Limit Agilent 5977A uses this sensitivity specification for detection techniques where no or little noise is generated, such as when OFN standard is injected. IDL is a new specification for SIM, but...

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IDL is a statistically derived value from the precision (%RSD) of 8 consecutive, splitless injections of 100fg OFN at 99 % confidence level. The more precise instruments (lower %RSD) will generate lower The Agilent 7890B/5977A with an ALS or CTC autosampler has the following specifications: These are installation specification, performed on customer site, immediately after installation and carry a lot more value than a reference specs. *Systems without an Autosampler will have only S/N specification.

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Why IDL specification is useful for the 5977 in SIM? Influence of Noise in S/N specs • In SIM (or MS/MS) when the Noise is too low, such as during acquisition of an OFN standard, S/N results may not give the best characterization • If the noise level is insignificant - varies a lot and too low- the derived value from it (S/N) also becomes insignificant Value of IDL • IDL is derived from reproducibility/precision results, the same metrics most users utilize every day in their work. The IDL statistical process is the same used for Method Detection Limit calculations, and the %RSD values are...

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ASK What’s New From Agilent GC/MS? ANSWER MassHunter for GC/MSD with MSD ChemStation DA 25 March 8, 2013

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MassHunter GC/MS Acquisition for GC/MSD Unified GC/MS Acquisition and MassHunter Data Analysis; with MSD ChemStation Workflow for GC/MSD Quant Qual Familiär GC/MS Acquisition Method Editor Integrated Calculators Agilent Technologies

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MassHunter GC/MSD Feb 2013: • GC/MSD Fundamentals – – – – 5975 & 5977 Series GC/MSD 7890B & 7820A 7697A HSS Current MassHunter GC/MS and GC products • MH DrugQuant • GC and GC/MSD Data Analysis with MassHunter OR MSD ChemStation modes

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All Inclusive G1701FA Software Package • Acquisition, Quant, Qual, MSD ChemStation •21CFR11 Compliance tools (Acquisition and • GC/MS and LC/MS Translators Collated User Information •Workflow guides, Video Tutorials, all in one • 1 yr Upgrade, Updates, and Phone Support • Installation and Familiarization options • New Familiarization material for GC/MSD

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