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| \^EasiCollect™ Consistent, reliable DNA collection—every time. | ||||||||
| Whatman | ||||||||
| EasiCollect is a novel development in the process of collecting buccal cell samples for genetic analysis. The EasiCollect system allows the uniform collection and application of cells to the surface of an FTA® Card for the capture of DNA. FTA® Cards provide a cost effective room temperature method for collecting, shipping, archiving, and processing nucleic acids from a wide variety of biological samples. FTA Cards inactivate pathogens for user safety and facilitate rapid purification of nucleic acids. Purification of nucleic acids on FTA Cards takes less than 30 minutes per sample when performed manually. With automation, time and labor are reduced and throughput is increased. The method produces high quality DNA for PCR and other downstream applications such as STR and SNP genotyping. | ||||||||
| Buccal cell collection, in the past, has been a notoriously difficult process. This has mainly been due to the differences in the ability of various individuals to shed cells. The reliability of most methods of buccal cell collection has always been a problem. There have also been issues with the ability of the cells to be deposited on a matrix in a uniform manner for reproducible sampling. Sampling an area of a matrix has occasionally missed deposits of cells. This has led to resampling, reprocessing and reamplification of samples costing time and money. There has clearly been a need for a more reliable way to collect and process buccal cell samples for genetic analysis. EasiCollect eliminates the difficulties of buccal cell collection. The unique design has been engineered for maximal cell collection and uniform application to FTA, the leading DNA collection and storage matrix. The unique foam collection surface has been specifically selected for its ability to capture the largest number of cells to overcome the individual differences. The pressure clips molded into the EasiCollect device ensures a uniform and constant pressure for the reproducible transfer of cells to FTA. The FTA Card chosen for the device is compatible with manual or automated punching systems which encompass all degrees of laboratory throughput. FTA is the market leader in DNA capture for genetic analysis. With over 17 years of DNA stability, EasiCollect and FTA are the system of choice for buccal cell collection and DNA stabilization. | ||||||||