Method

Protocol and Results 60 mL of tartrazine solution is poured into a manual fill reservoir. The reservoir is placed on position 2 of the Bravo. A 10 L tip box is placed on position 3. A 384-well polystyrene plate is placed on position 5. VWorks4 liquid classes for 0.3-0.5 L and 1-5 L dispenses are utilized. A VWorks4 protocol is created and run in the following manner: 1. Tips are pressed onto the head. 2. 0.3, 0.5 or 2.0 This story can fit 150-200 words. One benefit of using your newsletter as a promotional tool is that you can reuse content from other marketing materials, such as press releases, market studies, and reports. While your main goal of distributing a newsletter might be to sell your product or service, the key to a successful newsletter is making it useful to your readers. A great way to add useful content to your newsletter is to develop and write your own articles, or include a calendar of upcoming events or a special offer that promotes a new product. You can also research articles or find “filler” articles by accessing the World Wide Web. You can write about a variety of topics but try to keep your articles short. Much of the content you put in your newsletter can also be used for your Web site. Microsoft Pub-lisher offers a simple way to convert your newsletter to a Web publication. So, when you’re finished writing your newsletter, convert it to a Web site and post it. L tartrazine solution is aspirated from the manual fill reservoir. Aspirate parameters are 6 mm from the bottom of the plate, and preceded by a 0.4 Lpre-aspirate volume (1 L for 2 L dispense). 3. 0.3, 0.5 or 2.0 L tartrazine solution is dispensed into one quadrant of a 384-well plate. Dispense parameters are 0 mm from the bottom of the plate, and followed by dispensing a 0.4 L blowout volume (1 L for 2 L dispense). 4. The transfer is repeated for the other quadrants of the plate. Dispensed volumes are diluted to 50 L, compared to the actual absorbance value in each well. Absorbance values in each well is used to deter-mine the precision of the dispense. Coefficient of Variance (CV) calculations were made by dividing the standard deviation by the mean. The accuracy and precision results are typical, based on averages of three successive dispenses. Accuracy outliers are an average per plate. Results may vary, depending on individual experimental methods and liquid class optimization. Lby the addition of water (in this case, with clean tips and a separate VWorks4 protocol). Plates are centrifuged at 1800 rpm for 60 seconds to ensure full mixing and consistent well menisci. Absorbance is read at 405 nm. Accuracy is calculated based on an equation derived from a tartrazine/DMSO calibration curve consisting of data points at 0.1, 0.3, 0.5, 0.75, 1, 1.25, 1.5, 2, and 2.5

Results

Volume 0.3 L 0.5
Results L 2 L
Accuracy & Precision of the 96ST Head with 10 L Tips on the Bravo Platform Precision (% CV) 3.1 2.3 0.8 3 1.1 0.8 0 This story can fit 100-150 words. The subject matter that appears in newsletters is virtually endless. You can include stories that focus on current technologies or innovations in your field. You may also want to note business or economic trends, or make predictions for your customers or clients. If the newsletter is distributed internally, you might comment upon new procedures or improvements to the business. Sales figures or earnings will show how your business is growing. Some newsletters include a column that is updated every issue, for instance, an advice column, a book review, a letter from the president, or an editorial. You can also profile new employees or top cus-tomers or vendors.
Accuracy Precision % Accuracy (±) 1.5 Outliers (±5%) 7
Variance (<%) Outliers (±10%) 1 1 0
012345 0.00.51.01.52.02.53.0 Transfer time (seconds/ plate) 24 24 27
Volume ( L)

Conclusion

In summary, the liquid handling capabilities of the Bravo liquid handling platform in conjunction with a 96 Channel ST Disposable Tip Head provides precise, accurate and consistent liquid transfers. The 96 channel head, combined with Velocity11’s 10
Conclusions L disposable tips, can pipette across a low volume range (0.3-10 L), making it ideal for applications requiring precise dispensing at low volumes. The highly configurable deck of the Bravo liquid handling platform along with its compact footprint makes it ideal for many applications in the labora-tory including compound sample transfers, genomic applications, cell plating, and cell-based assays. References: 1. Sofia MJ. Leveraging Compound Management Capabilities in Support of Drug Discovery: From Sample Archive to Sample Distribu-tion — Driving Efficiency and Improving Productivity. Laboratory Robotics Interest Group Meeting Oral Presentation, Jan. 2005. 2. Holman JW, Mifflin TE, Felder RA, Demers LM. Evaluation of an automated preanalytical robotic workstation at two academic health centers. Clin Chem. 2002 Mar; 48(3):540-8.
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