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| | | | | | | | | | | | | | | | | | | | | | | | | | | | | Bond Elut Cartridge SPE Bond Elut® Cellulose | | | | | | Bond Elut® CBA | | | | | | | | | | • Base silica: 40 urn or 120 urn irregularly-shaped, acid-washed silica, 60 A mean pore size • Bonded functional group: carboxylic acid • Sorbent pKa: 4.8 • Mean capacity: 0.35 meq/g • Endcapped:yes • Typical percent carbon loading: 7.4% • Primary retention mechanism: weak cation exchange • Typical sample types: water, biological fluids | | | | | | The Varian Bond Elut Cellulose column utilizes a pure microgranular cellulose powder that is packed between two 20 um polyethylene frits in an ultraclean 3 mL polypropylene extraction column. The unique rod-like shape and controlled particle size of this variety of Cellulose ensure that it is free-flowing when dry. In addition, the material is manufactured from high purity cotton with a very high a-cellulose content that ensures optimum column packing properties. Thus, high density, more uniform, smooth-packed column beds are achieved. In addition, the cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and Cu content less than 5 ppm). The combination of surface area and polymeric structure results in a sorbent with excellent capacity. The cellulose media contains numerous hydroxyl groups. Because of its polar nature, it is able to accept high loading of many polar substances from aqueous and organic phases. The free hydroxyl groups' binding properties are complemented by the presence of very small quantities of residual aldehydes, ketones and carboxyl groups. These weak groups add reducing and ion-exchange properties. This microgranular cellulose support has been utilized for large professional scale bioprocessing as well as a prefilter for suspended matter (i.e. cell debris and suspended fats), as a prefractionation step prior to reverse phase HPLC, for separation of biomolecules, and as an affinity chromatography matrix. For assistance selecting the best sorbent for your application, please see the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and use our Applications Online Scanview database. Bond Elut Straight Barrel Cartridges | | | | | | | | | | q. m S m m q. in Q) ^_ CL | | | | | | Bond Elut CBA carboxylic acid sorbent CBA is a medium polarity sorbent based on carboxy propyl functionalized silica, exhibiting either polar or nonpolar interactions depending on the matrix or solvent environment. Its prime characteristic is its weak cation exchange properties. Because of its pKa value of 4.8, CBA does not require extreme basic conditions for elution that are required by other cation exchange sorbents. Above a pH of 4.8, the majority of functional groups carry a negative charge that can be used for retaining cationic compounds. Dropping the pH to 2.8 effectively neutralizes CBA, permitting the retained analytes to elute from the sorbent. For this reason, CBA is often the best choice for cation exchange, especially when dealing with very strong cations (i.e. high pKa cations). In addition, CBA shows a wider range of selectivity towards counter-ions than other cation exchange phases, enabling more options for elution. For assistance selecting the best sorbent for your application, please see the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and use our Applications Online Scanview database. | | | | | | | | | | | | | | Bond Elut LRC Cartridges | | | | | | | | | | | | | | | | | | | | Sorbent Mass (mg) | Volume (mL) | 40 urn | 120 um | Quantity (/Box) | | | | 100 | 10 | 12113011 | 14113011 | 50 | | | | 500 | 10 | 12113037 | 14113037 | 50 | | | | Bond Elut Straight Barrel Cartridges | | | | Sorbent Mass | Volume (mL) | 40 um | 120 um | Quantity (/Box) | | | | 50 mg | 1 | 12102073 | | 100 | | | | 100 mg | 1 | 12102011 | 14102011 | 100 | | | | 3 | 12102097 | | 50 | | | | 200 mg | 3 | 12102124 | | 50 | | | | 500 mg | 3 | 12102038 | 14102038 | 50 | | | | 1 g | 6 | 12256009 | 14256009 | 30 | | | | 2 g | 12 | 12256058 | | 20 | | | | | | | | | | | | | | | | | | | | | | | | Sorbent Mass (mg) | Volume (mL) | Part No. | Quantity (/Box) | | | | 300 | 3 | 12102095 | 500 | | | | | | | | | | | | | | | | | | | | | | | | | | | Bond Elut Cartridges with Stainless Steel Frits | | | | | | | | | | | | | | | | Sorbent Mass (mg) | Volume (mL) | 40 um | 120 um | Quantity (/Box) | | | | 100 | 1 | 12124011 | 14124011 | 100 | | | | 500 | 3 | 12124035 | 14124035 | 50 | | | | | | | | | | | | | | | | | | | | See All of Our Products, and Shop Online at www.varianinc.com | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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