Nano ITC Isothermal Titration Calorimeter - 9 Pages

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Nano ITC Isothermal Titration Calorimeter
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Catalogue excerpts

New Castle, DE USA Lindon, UT USA Elstree, United Kingdom Shanghai, China Beijing, China Taipei, Taiwan Tokyo, Japan Seoul, South Korea Bangalore, India Paris, France Eschborn, Germany Brussels, Belgium Etten-Leur, Netherlands Sollentuna, Sweden Milano, Italy Barcelona, Spain Melbourne, Australia Mexico City, Mexico

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ISoTherMal TITraTIon Calorimetry With the Nano ITC, heat effects as small as 100 nanojoules are detectable using one nanomole or less of biopolymer. The Nano ITC uses a solid-state thermoelectric heating and cooling system to precisely control temperature, and a unique removable syringe assembly for efficient and accurate delivery of titrant. The true isothermal power compensation design of the Nano ITC delivers ultra fast response times. The TA Instruments Nano ITC is engineered specifically for binding and kinetics studies on purified dilute biological samples of limited availability

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Specs Temperature Range Temperature Stability Minimum detectable heat Maximum detectable heat Baseline Stability Noise Level Response Time Cell Volume Cell Conguration Cell Material Injection Syringe Volumes Standard Volume 2 to 80 °C ±0.0002 °C @ 25 °C 0.1 μJ 5,000 μJ ±0.02 μW/hr 2.5 nW 13 Seconds* 1.0 ml Fixed-in-place, Cylindrical 24K Gold* or Hastelloy 100 μL and 250 μL 2 to 80 °C ±0.0002 °C @ 25 °C 0.05 μJ 3,000 μJ ±0.02 μW/hr 1.4 nW 11 Seconds 190 µL Fixed-in-place, Cylindrical 24K Gold 50 μL

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ITC teChNology The unique removable syringe assembly contains a mechanical paddle stirrer at the end, the speed of which is easily adjusted to accommodate the physical properties of the sample. The integrated titration assembly of the Nano ITC ensures quick-filling, simple cleaning and accurate titrations. The Nano ITC Standard Volume is available with sample cells made from 99.999 % Gold or Hastelloy C to allow for the widest range of reagent chemistry. The Nano ITC Low Volume is available with sample cell cells made from 99.999 % Gold. Life Science professionals know that the thermodynamic driving...

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The Nano ITC instrument is available in two sample cell sizes. The Nano ITC Standard Volume sample cell volume is 1.0 ml. The Nano ITC Low Volume offers the lowest cell volume at 190 uL, to minimize sample consumption and at the same time provides sensitivity levels over two times better than previously achievable. Heat effects as small as 50 nanojoules are detectable in the Nano ITC Low Volume with a short term noise level of 1.4 nanowatts. Both Nano ITC instruments use cylindrical-shaped cells to make cleaning easy, solid state thermoelectric heating and cooling systems to precisely control...

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ITC aPPliCatioNS Characterization of Enzyme Kinetics Continuous single injection titration is an attractive alternative to the traditional incremental titration ITC for samples exhibiting very rapid binding reactions. These continuous injection experiments can be completed in less total time than normally required for a full set of incremental titrations. This technique provides accurate determinations of stoichiometry (n) and enthalpy (ΔH) for a wide range of binding constants. Continuous injection and incremental injection experiments can be performed in both the Nano ITC standard volume and...

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reFereNCeS Garbett, N., DeLeeuw, L. and J.B. Chaires. MCAPN-2010-04. High-throughput DSC: A Comparison of the TA Instruments Nano DSC Autosampler System™ with the GE Healthcare VP-Capillary DSC™ (2010) Demarse, N. and L.D. Hansen. MCAPN-2010-01. Analysis of Binding Organic Compounds to Nanoparticles by Isothermal Titration Calorimetry (ITC). (2010) Quinn, C.F. MCAPN-2010-02. Analyzing ITC Data for the Enthalpy of Binding Metal Ions to Ligands. (2010) Quinn, C.F. and L.D. Hansen. MCAPN-2010-03. Pressure Perturbation Calorimetry: Data Collection and Fitting. (2010) Román–Guerrero, A.,Vernon–Carter,...

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© 2012 TA Instruments. All rights reserved.

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