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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e
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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 1

Comprehensive Two-Dimensional Liquid Chromatograph

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 2

Nexera-e Comprehensive Two-Dimensional Liquid Chromatograph Novel Separation Method for Complicated Sample Matrices The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible theoretical plates are achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially...

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 3

Comprehensive Two-Dimensional Liquid Chromatograph

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 4

New Paradigm Shift in Liquid Chromatography Nexera-e Maximizing the full p g potential of two-orthogonal separation systems to g p y perform co mpre hens ive two-dimensional separation perf orm comprehensive two dime nsio nal sepa rati on pe rfor m comp rehe nsiv e tw o -di mens iona l se para tion Comprehensive 2D-LC is a new analytical methodology Unlike other conventional two-dimensional techniques the combining two independent separation modes orthogonally, Nexera-e provides complete separation as a result of the greatly increasing separation efficiency. The combination of combination...

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 5

S e p a ra tio n fa r e xce e din g co nve n tio n al 1D - LC How many peaks are co-eluted under one peak? The more co-eluting compounds may be unable to be separated by complex the sample, along with a high percentage of similar mono-dimensional separation, the orthogonal compounds, means there is a high probability of co-eluting two-dimensional system will provide the best possible result. compounds found under single peaks. Even though the Unseparated peaks Second-dimensional separation Peaks separated with two separation systems Monodimensional separation First-dimensional separation...

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 6

Nexera-e Components Adoption of Nexera X 2 components of fering ultimate per formance Comprehensive 2D-LC requires excellent performance from requirements with its high-performance Nexera X2 each component. For example, the 2nd-dimensional separation components. For example, the solvent delivery unit, LC-30AD, system requires ultra-first gradient analysis with a high flow provides solvent delivery at a flow rate up to 3 mL/min with a rate and ultra-high pressure. Moreover, in order to analyze pressure limit of 130 MPa. In addition, the ultra-high-pressure differences between samples,...

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 7

ChromSquare, the data analysis software for 2D-LC □ 2D Qualitative & Quantitative analysis using Contour Graphics Acquired data is converted to two-dimensional contour plotting using ChromSquare, the software for comprehensive 2D-LC analysis. A peak on chromatogram is recognized as spot or feature on the contour plot. The qualitative and quantitative data processing are performed for the target spot. LCxLC/MS data analysis of standard phospholipid sample Cupper left: Contour plot (magnified view); lower left: MS spectrum; upper right: Second-dimensional chromatogram; lower right: Contour...

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Comprehensive Two-Dimensional Liquid Chromatograph Nexera-e - 8

Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”. Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own. For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are...

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