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Evolve Delta EMCCD for Biomedical Research - 4 Pages

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Catalogue excerpts

Evolve® 512 Delta EMCCD CAMERA After years of engineering expertise that perfected every element of the original Evolve 512 camera, Photometrics continues to push technology boundaries. The Evolve 512 Delta OEM is the most advanced EMCCD technology in the Photometrics product portfolio, providing instrumentation builders a significantly advanced, life science imaging offering. Designed specifically for demanding super resolution applications, the Evolve 512 Delta OEM camera delivers an unprecedented new level of speed and sensitivity. The camera is available with a revolutionary new LightSpeed mode which maximizes image acquisition rates for dynamic live cell, highspeed events by enabling frame rates of up to 3,000 frames per second (fps) The Evolve 512 Delta OEM also provides superior quantitative and detection performance, with accurate and precise triggering capability. Camera control is provided through the standard Photometrics PVCAM API. Instrument builders have chosen to offer this camera as a high performance upgrade to their instruments based on routine PVCAM cameras. Back-Thinned EMCCD Sensor Extreme sensitivity to detect single photon signals Electron Multiplication to minimize read noise <1 electron Large pixels to maximize signal collection ability High Speed Triggering Precise timing with complex systems, controlling illumination, shutters and filter wheels, and other triggered devices Multiple Read-Out Speeds Optimize your acquisition to your experiment High speed imaging at 20MHz providing 67 frames per second Video Rate imaging at 10MHz Standard CCD Imaging Mode 5MHz and .625MHz speeds available for time-lapse imaging LightSpeed Mode Maximize image acquisition rate for highly dynamic events Arbitrary ROIs enabled for flexibility in selecting targeted areas Simultaneous dual channel imaging at extremely high frame rates when used with the DV Lambda and DV2 Image a 128x128 array at 794 frames per second Extremely stable EM Gain performance over the life of the camera Most accurate and precise integrated EM Calibration routine using a highly stable integrated light source Calibrates your EM gain in less than three minutes Highly-Stabilized Cooling Ensures consistent and accurate EM Gain performance and virtually eliminates dark current generation Performance SMART Streaming™ Faster acquisition rates with variable exposures, ideal for multi-probed live cell imaging Select up to 12 unique exposure times PAR™ Feedback System Photometrics Active Regulation, delivers unsurpassed EM gain stability for outstanding signal fidelity Advanced Clocking Enhancement, provides lowest noise floor and minimizes generation of spurious charge and background events

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512 x 512 imaging array, 16 x 16-μm pixels = Optimized field of view and = Wide dynamic range allows highest sensitivity = Single Molecule Fluorescence = Ratiometric Ion Imaging detection of bright and dim signals in the same image = Spinning Disc Confocal Microscopy = Small Animal Imaging Frame-transfer EMCCD = 100% duty cycle to collect continuous data = No mechanical shutter microscopes, standard lenses, or optical Exclusive eXcelon Technology (optional) Turbo-1394™ interface (IEEE-1394a) Quantum Efficiency Curve 100% interface that provides high-bandwidth, uninterrupted data transfer with...

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Array Size Pixel Size Single Pixel Full-Well Read Noise (e- rms) 20 MHz EM Port 10 MHz EM Port 5 MHz Standard Port .625 MHz Standard Port Bias Stability A measurement of the camera stability when no light hits the sensor. A slope of zero would be ideal. (See footnote #1) Stabilized Cooling Temperature Air cooled (@ ambient air 20˚C) - Standard - 75˚C Liquid cooled (@ ambient air 20˚C) - Optional - 75˚C Dark Current Background Events (20 MHz, 1000X EM gain) Standard operation Parallel Shift Rate The shift rate is optimized for maximum frame rates while providing extremely high charge transfer efficiency...

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(Frames per second) Note: Frame rates are measured at 20 MHz with 0-second exposure times. LightSpeed Mode Frame Rates Single Channel 256 x 256 application needs branding options (Frames per second) Note: Frame rates are preliminary. For the latest published frame rates visit www.photometrics.com Unique part number/Bill of Materials (BOM) CCD CENTERED TO +/- (2) PIXELS FROM CENTERLINE Single driver platform supports a wide range of product offerings Strategically located global service centers Dedicated support from a focused OEM team #1 Bias stability – The imaging stability of the EMCCD camera...

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