Positioning Tasks in Microscopy Endothelial cells as seen under the microscope. Source: Lemke Group, EMBL Heidelberg Almost all microscopic techniques have similar basic requirements in regard to the positioning elements: compact design and very high accuracy. Often, many individual images are to be taken during the examination of a sample in as short a time as possible or dynamic processes are to be observed – both tasks place great demands on the dynamics of the stage. However, if one considers the requirements of individual applications more closely, it becomes apparent that the details of...

XY Scan of Large Samples If larger samples have to be examined (e. g. well plates), stages with an XY travel range of approx. 130 x 80 mm and a large clear aperture of 160 x 110 mm are typically required. For these applications PI offers highly compact systems with the innovative PILine piezo linear drives (p. 24). PInano XY(Z) scanning system with additional M-545 manual stage ® requires an adjustment range of approx. 25 mm in both axes. A good position stability of the stages as well as minimum play are important here. Naturally, the stages also have to fit on the corresponding microscopes....

Drift compensation XY and XYZ Tracking with High Dynamics Sample Positioning If individual molecules are to be examined, a fast positioning system is required that can keep up with the small but very rapid and stochastically distributed molecule movements. In these applications, a small travel range (approx. 50 μm) is usually sufficient but in return much higher dynamics are required. The P-545.3D7 PInano® track system with its settling time of typically 5 ms (p. 9) is well suited to this purpose. For sample positioning, PI offers PInano® Z piezo stages – either with a 100 or 200 μm stroke (p....

PIFOC® system with QuickLock inserts and E-709 controller (numeric aperture), there are PIFOC® objective positioners with a large clear aperture (up to 29 mm with a M32 thread). The PIFOC® positioners are driven with a digital controller (p. 22), which can be operated via an analog input or digital interfaces (USB, RS-232). The system, consisting of a digital controller and a PIFOC® positioner with capacitive feedback, is characterized by maximum flexibility, easy operation and it is also more favorably priced than previous s ­ ystems that use analog electronics (p. 27). Autofocus or Drift Compensation...

PInano® Cap XY(Z) Piezo System C A PA C I T I V E P O S I T I O N I N G M E A S U R E M E N T F O R S U P E R - R E S O L U T I O N M I C R O S C O P Y P-545.xC7 ighest stability and H repeatability ravel ranges up to T 200 × 200 × 200 µm3 ub-nanometer resolution S s-response times m ow Profile for easy L integration: 20 mm ecessed slide holder, R free rotation of turret Reference-class system: high-resolution piezo stage for 3×1“ object slides USB controller and software included PICMA® high-performance piezo drive Frictionless flexure guiding system, FEM-optimized flexure joints. Piezo actuators...

Integrated sensor Closed-loop travel Closed-loop resolution* Push / pull force capacity Recommended load** Piezo ceramics Electrical capacitance Cable length Piezo Controllers Communication interfaces Control input socket User software Software drivers LabVIEW drivers, shared libraries for Windows and Linux Supported functionality Wave generator, data recorder, macro programming, auto zero, trigger I/O, MetaMorph, µManager, MATLAB Operating temperature range Controller dimensions Mechanical properties Linear Actuators & Motors Piezo Scanning Systems 2- and 3-Axis Drive properties Accessories:...

PInano® XY(Z) Piezo System LOW-COST NANOPOSITIONING SYSTEM FOR SUPER-RESOLUTION MICROSCOPY P-545.xR7 Tost-effective design due to piezoresistive sensors Travel ranges up to 200 × 200 × 200 µm3 Low profile for easy integration: 20 mm Large clear aperture for 3x1‘‘ object slides, recessed sample holders Outstanding lifetime due to PICMA® piezo actuators Sub-nanometer resolution, ms-response times Precision-class positioning system USB controller and software included PICMA® high-performance piezo drive Frictionless flexure guiding system. FEM-optimized flexure joints. Piezo actuators with ceramic...

PInano® Trak Piezo Tracking System FAST XY(Z) STAGE FOR HIGH-DYNAMICS MICROSCOPY Precision-class system: fast positioning XY(Z) stage USB controller and software included Fast response < 5 ms with sub-nanometer resolution: ideal for tracking Travel ranges up to 70 × 70 × 50 µm3 Low profile for easy integration: 20 mm Recessed slide holder, free rotation of turret Units Tolerance PICMA® direct drive High-performance piezo actuators without amplification for fastest response. Piezo actuators with ceramic insulation for an outstanding lifetime Integrated sensor Closed-loop travel Closed-loop resolution*...

Linear Actuators & Motors PInano® Piezo Controller Nanopositioning & Piezoelectrics THREE CHANNELS WITH USB INTERFACE Controller for P-545 PInano® piezo nanopositioner With fast digital and analog interfaces. For piezoresistive strain sensors (PRS) or capacitive sensors TCP/IP, USB, RS-232 and three analog interfaces Sensor type Servo characteristics P-I (analog), notch filter P-I (analog), notch filter Peak output current, < 5 ms Average current Supported software MetaMorph, µManager, MATLAB Piezo Servo Controller for PInano® positioner Current limitation Voltage gain Functions General Command...

Linear Actuators & Motors Open-Frame Microscope Stage Nanopositioning & Piezoelectrics LOW PROFILE, LOW DRIFT DESIGN, LONG TRAVEL RANGE Standard-class, manual XY microscope stage Coarse adjustment for P-545 piezo nanopositioning systems. Stiff design enables optimal scanning and settling behavior Micrometer screws, motor upgrade available For inverted microscopes made by Nikon, Zeiss, Leica, and Olympus Accessories M-545.USG M-229 Stepper-Mike Upgrade for M-545 Stages: Includes Stepper-Mikes M-545.SHP Adapter Plate for Microscope Sample Holder for M-545 XY Microscope Stage M-545.USC Stepper-Mike...