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Gated Spectrometer for Fluorescence

Ultimate Fluorescence Spectrometer

The USB2000-FLG Spectrofluorometer is apreconfigured spectrometer for fluorescence applications from 380-1050 nm. We utilize a proprietary thin film technology and a time- gated spectrometer to provide a spectro- fluorometer that is 20 percent more sensitive than the standard USB4000 Spectrometer.

Avoiding Scattered Light

In addition to detecting fluorescence from a sample, a spectrofluorometer will also detect scattered excitation light from the light source and the sampling compartment. (Scattering is especially prevalent in turbid samples.) The USB2000-FLG uses two features to avoid detecting scattered light: special mirrors in the optical bench to absorb ultraviolet light and improve sensitivity and -- for fluorophores with long fluorescence lifetimes -- a time-gated mode that delays the start of spectral data acquisition by 5-500 microseconds after the excitation source is turned on (or pulses).
In addition to theUSB2000-FLG, a common fluorescence setup may include products like those shown above: the PX-2 Pulsed Xenon Source, the CUV-ALL-UV Cuvette Holder, LVFLinear Variable Filters, and optical fibers. Spectrometer Systems & Setups

Gated Fluorescence Mode

The USB2000-FLG is preloaded with variable- delay gating microcode, which allows you to select a delay (from 5-500 microseconds) in our software between the light turning on and the start of the spectrometer's integration time, when the detector “sees” the sample. In this Gated Mode, the spectrometer only detects the sample light when the source is off. In order to use the Gated Mode, your fluorophore must have a long fluorescence lifetime. Those working with lanthanides and photoluminescent materials will find this mode very useful. The best excitation source to use with the Gated Mode is our PX-2 Pulsed Xenon Source.
Terbium-dipicolinic acid (Tb-DPA) photoluminescence spectra acquired with the USB4000-FLG while in the Gated Mode, with the data acquisition delay set at a range of values. Analysis of samples with long emission lifetimes such as Tb-DPAimproves when data acquisition is delayed until the energy from the lamp pulse is no longer observed in the spectrum, resulting in a cleaner, more resolved Tb-DPAspectrum -- achieved without using optical filters. At delay times below 40 microseconds, lingering excitation energy and background fluorescence overlap the Tb-DPAphotoluminescence spectrum.

SAG+ High-reflectivity Mirrors

In the USB2000-FLG, we replaced our standard mirrors with proprietary Ag-coated mirrors to increase reflectance, which increases the sensitivity of the spectrometer by more than 20 percent. They also absorb nearly all ultraviolet light, virtually eliminating the excitation source's spectra from interfering with the sample spectra.
Specifications Dimensions:89.1 mm x 63.3 mm x 34.4 mmWeight:190 g Power consumption:90 mA@ 5 VDC Wavelength range:380-1050 nm Detector:2048-element linear silicon CCD arrayGrating:Grating #3 -- 600 lines per millimeter, blazed at 500 nmEntrance aperture:200 µm wide slit Optical resolution:~10.0 nm FWHM Stray light:~0.05% at 600 nm, <0.10% at 435 nm Fiber optic connector:SMA905 to 0.22 numerical aperture single-strand fiberIntegration time:Gated mode - 5 milliseconds, normal mode - 3 milliseconds to 60 secondsOperating systems:Windows 98/Me/2000/XP, Mac OS X and Linux operating systems whenusing the USB port; any 32-bit Windows OS when using the serial port USB2000-FLG: $2,999

Application Flexibility

The USB2000-FLG can detect fluorophores in solutions and powders, and from surfaces. The USB2000-FLG has been used to measure fluorescence in coral, fruit and other flora and fauna.
Tel:727.733.2447 • Email:Info@OceanOptics.com47

Catalog Ocean Optics - 18485 oo oo