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QE65000 Configured for Fluorescence
Scientific-grade Spectrometer
The QE65000 Spectrometer is a unique combination of detector and optical bench technologies that provides users with high spectral response and high optical resolution in one scientific-grade spectrometer package.
Demanding Low Light-level Applications
The QE65000 was designed for low-light level applications such as fluorescence, Raman spectroscopy, DNA sequencing, astronomy and thin-film reflectivity. For a complete fluorescence system, combine the QE65000 with a PX-2 Pulsed Xenon Light Source (page 127) to excite your sample in a CUV-ALL 4-Way Cuvette Holder (page 90). In addition, we offer LVF Linear Variable Filters (page 114) to spectrally shape excitation energy from broadband sources such as the PX-2. A solid secondary fluorescence standard, the STAN-FL-RED, can be used for quick and easy calibration, stability, and performance checks of your fluorescence system.
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PX-2 Pulsed Xenon Light Source
QE65000 Spectrometer
Specifications
PHYSICAL
Dimensions (in mm): 182 x 110 x 47
CD <
CD
(D
3 CD
Weight: DETECTOR
1.05 kg
Detector:
Hamamatsu S7031-1006 back-thinned CCD (page 28)
Detector range: 200-1100 nm
Pixels:_1024 x 58 (1044 x 64 total)
Pixel size: 24.6 pm square size
Signal-to-nolse ratio: 1000:1 (at full signal) Dark noise: 2.5 RMS counts
Quantum Efficiency to 90%
The Hamamatsu FFT-Ccd detector used in the QE65000 achieves up to 90% quantum efficiency (defined as how efficiently a photon is converted to a photoelectron). Most of our other detectors are linear CCDs, but with this "2D" area detector we can bin a vertical row of 64 pixels that offers significant improvement in the signal-to-noise ratio (1200:1) and signal processing speed of the detector compared with a linear CCD, where signals are digitally added by an external circuit.
Increased System Sensitivity
QE65000 system sensitivity is improved because the 2D detector allows us to take advantage of the height of the entrance slit. In our spectrometers, you regulate the light entering the bench according to the slit's width. Most of our other spectrometers use linear detectors; in those spectrometers, slit height doesn't matter because linear detectors cannot efficiently collect the light from the entire height of the slit. But with the 2D detector in the QE65000, we can better take advantage of this additional light, extremely useful in low-light fluorescence applications.
QE65000: $9,999
PX-2: $769
CUV-ALL: $809
STAN-FL-RED: $179
Sensitivity:
400 nm: 22 electrons/count, 250 nm: 26 photons/count
OPTICAL
BENCH
CD (D
C TJ CD
Design: Focal length:
f/4, Symmetrical crossed Czerny-Turner 101.6 mm input, 101.6 mm output
Entrance aperture: 5, 10, 25, 50, 100, or 200 urn wide slits (page 27) 14 gratings, UV through Shortwave NIR (page 28) OFLV-QE and OF-1 order sorting filters (page 27)
G
SPECTROSCOPIC
Wavelength range: Grating dependent
Optical resolution: -0.14-7.7 nm FWHM_
Integration time: 8 milliseconds to 15 minutes
Dynamic range:_25000:1 a single acquisition; 7.5 x 109 (system)_
Stray light:_<0.08% at 600 nm, <0.4% at 435 nm_
Fiber optic connector manufacturer: SMA 905 to 0.22 numerical aperture single-strand fiber ELECTRONICS
Power consumption: 500 mA @ 5 VDC no TE cool;
_3 A @ 5 VDC with TE cool_
Data transfer speed: Full spectrum to memory every 8 ms with USB 2.0 port,
8 ms with USB 1.1 port_
10 onboard digital user-programmable GPIOs_
TEMPERATURE & THERMOELECTRIC (TE) COOLING_
Temperature limits: 0 °C to 50 °C for spectrometer, no condensation Temperature range: 13 °C maximum range between the high and low
Set point:_ Software controlled
Lowest set point:
40 °C below ambient, to -15 °C ±0.1 °C of set temperature in <2 minutes
Stability:
COMPUTER
Operating systems: Windows 98/Me/2000/XP, Mac OS X and Linux when using the USB port; 32-bit Windows OS when using
_the serial port_
Computer interfaces: USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115K baud Peripheral interfaces: SPI (3-wire); I2C inter-integrated circuit_
45
Tel: 727.733.2447 • Email: Info@OceanOptics.com
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